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Mouse whole mount RNA in situ hybridization: an effective technique for analyzing gene expression.

Abstract To set up a method of analyzing gene expression profile from mouse whole embryos.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title zhonghua yi xue yi chuan xue za zhi = zhonghua yixue yichuanxue zazhi = chinese journal of medical genetics
Publication Year Start




PMID- 14767909
OWN - NLM
STAT- MEDLINE
DCOM- 20040429
LR  - 20100323
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 21
IP  - 1
DP  - 2004 Feb
TI  - [Mouse whole mount RNA in situ hybridization: an effective technique for
      analyzing gene expression].
PG  - 47-51
AB  - OBJECTIVE: To set up a method of analyzing gene expression profile from mouse
      whole embryos. METHODS: Mouse whole mount RNA in situ hybridization(WM-ISH) of
      E10.5-E14 embryos was carried out by using digoxigenin-labeled Runx1 and Runx3
      RNA probes and their expression profile was observed by detecting the existence
      and status of corresponding mRNAs in the embryonic tissues. RESULTS: Clear
      hybridization signals were observed in different tissues and organs hybridized by
      Runx1 or Runx3 RNA probe. Different probes and ages of embryos had need of their 
      own optimal proteinase K treatment conditions. CONCLUSION: Mouse whole mount RNA 
      in situ hybridization is an effective method of analyzing gene expression. It is 
      useful for revealing whole gene expression profile and has a great potentiality
      in the era of functional genomics. It provides an alternative method of studies
      on gene expression which is at least as good as LacZ staining and
      immunohistochemistry. The key factor of the success to mouse whole mount RNA in
      situ hybridization is whether the proteinase K treatment conditions are optimal
      or not.
FAU - Xiao, Cui-ying
AU  - Xiao CY
AD  - Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu,
      Sichuan, PR China. [email protected]
FAU - Levanon, Ditsa
AU  - Levanon D
FAU - Zhang, Si-zhong
AU  - Zhang SZ
FAU - Groner, Yoram
AU  - Groner Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese
      journal of medical genetics
JID - 9425197
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Core Binding Factor Alpha 3 Subunit)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Runx1 protein, mouse)
RN  - 0 (Runx3 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Core Binding Factor Alpha 3 Subunit
MH  - DNA-Binding Proteins/genetics
MH  - Embryo, Mammalian/*metabolism
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation, Developmental
MH  - In Situ Hybridization/*methods
MH  - Mice
MH  - Proto-Oncogene Proteins/genetics
MH  - RNA/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Sensitivity and Specificity
MH  - Transcription Factors/genetics
EDAT- 2004/02/10 05:00
MHDA- 2004/04/30 05:00
CRDT- 2004/02/10 05:00
PHST- 2004/02/10 05:00 [pubmed]
PHST- 2004/04/30 05:00 [medline]
PHST- 2004/02/10 05:00 [entrez]
AID - 940621012 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Feb;21(1):47-51.