PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Marek's disease virus-induced immunosuppression: array analysis of chicken immune response gene expression profiling.

Abstract Marek's disease (MD) is a lymphoproliferative disease of chickens induced by a highly cell-associated oncogenic alpha-herpesvirus, Marek's disease virus (MDV). MDV replicates in chicken lymphocytes and establishes a latency infection within CD4(+) T cells. Host-virus interaction, immune responses to infection, and transcriptional profiling of chicken gene expression in MD are poorly understood. In this study we conducted a global host gene expression analysis in the splenocytes of MDV-infected chickens using oligonucleotide-based Affymetrix GeneChip Chicken Genome Arrays. These arrays contain probes for more than 32,000 chicken transcripts and most of the known MDV genes and open reading frames. Two-week-old MD-susceptible chickens were inoculated with an oncogenic strain of MDV, and spleen samples were collected 5 and 15 days post-infection (dpi) for RNA isolation and microarray analysis. Array results displayed a significant differential pattern of immune response transcriptome between the two phases of MDV infection. The expression levels of more than 22 immune-response and related genes were downregulated, while the expression levels of at least 58 genes were increased at 5 dpi (cytolytic infection), compared to age-matched control birds. In comparison, out of 73 immune-response and related genes, 67 genes were downregulated, with only 6 genes having higher expression levels at 15 dpi (latency infection). Cytokines, chemokines, MHC molecules and related receptors, and adhesion molecules were among the many MDV-induced downregulated genes that are critical for an effective antiviral immune response. In addition, several apoptosis-associated genes were decreased in expression during latent infection, suggesting an MDV-induced blocking of initiation or progression of programmed cell death processes. These chicken arrays are valuable tools in understanding the molecular mechanisms behind viral pathogenesis and chicken gene expression patterns, and associated biological pathways in response to MDV infection.
PMID
Related Publications

Gene expression profiling in rMd5- and rMd5deltameq-infected chickens.

Marek's disease virus induces Th-2 activity during cytolytic infection.

Temporal transcriptome changes induced by MDV in Marek's disease-resistant and -susceptible inbred chickens.

Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek's disease virus.

Transcriptional profiling of Marek's disease virus genes during cytolytic and latent infection.

Authors

Mayor MeshTerms

Gene Expression Profiling

Genes, MHC Class II

Keywords
Journal Title viral immunology
Publication Year Start




PMID- 20565294
OWN - NLM
STAT- MEDLINE
DA  - 20100622
DCOM- 20100920
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 23
IP  - 3
DP  - 2010 Jun
TI  - Marek's disease virus-induced immunosuppression: array analysis of chicken immune
      response gene expression profiling.
PG  - 309-19
LID - 10.1089/vim.2009.0079 [doi]
AB  - Marek's disease (MD) is a lymphoproliferative disease of chickens induced by a
      highly cell-associated oncogenic alpha-herpesvirus, Marek's disease virus (MDV). 
      MDV replicates in chicken lymphocytes and establishes a latency infection within 
      CD4(+) T cells. Host-virus interaction, immune responses to infection, and
      transcriptional profiling of chicken gene expression in MD are poorly understood.
      In this study we conducted a global host gene expression analysis in the
      splenocytes of MDV-infected chickens using oligonucleotide-based Affymetrix
      GeneChip Chicken Genome Arrays. These arrays contain probes for more than 32,000 
      chicken transcripts and most of the known MDV genes and open reading frames.
      Two-week-old MD-susceptible chickens were inoculated with an oncogenic strain of 
      MDV, and spleen samples were collected 5 and 15 days post-infection (dpi) for RNA
      isolation and microarray analysis. Array results displayed a significant
      differential pattern of immune response transcriptome between the two phases of
      MDV infection. The expression levels of more than 22 immune-response and related 
      genes were downregulated, while the expression levels of at least 58 genes were
      increased at 5 dpi (cytolytic infection), compared to age-matched control birds. 
      In comparison, out of 73 immune-response and related genes, 67 genes were
      downregulated, with only 6 genes having higher expression levels at 15 dpi
      (latency infection). Cytokines, chemokines, MHC molecules and related receptors, 
      and adhesion molecules were among the many MDV-induced downregulated genes that
      are critical for an effective antiviral immune response. In addition, several
      apoptosis-associated genes were decreased in expression during latent infection, 
      suggesting an MDV-induced blocking of initiation or progression of programmed
      cell death processes. These chicken arrays are valuable tools in understanding
      the molecular mechanisms behind viral pathogenesis and chicken gene expression
      patterns, and associated biological pathways in response to MDV infection.
FAU - Heidari, Mohammad
AU  - Heidari M
AD  - United States Department of Agriculture, Agriculture Research Service, Avian
      Disease and Oncology Laboratory, East Lansing, MI 48823, USA.
      [email protected]
FAU - Sarson, Aimie J
AU  - Sarson AJ
FAU - Huebner, Marianne
AU  - Huebner M
FAU - Sharif, Shayan
AU  - Sharif S
FAU - Kireev, Dmitry
AU  - Kireev D
FAU - Zhou, Huaijun
AU  - Zhou H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
SB  - IM
MH  - Animals
MH  - Chickens
MH  - *Gene Expression Profiling
MH  - *Genes, MHC Class II
MH  - Immune Tolerance
MH  - Leukocytes, Mononuclear/immunology
MH  - Mardivirus/*immunology/*pathogenicity
MH  - Marek Disease/*immunology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Spleen/immunology
EDAT- 2010/06/23 06:00
MHDA- 2010/09/21 06:00
CRDT- 2010/06/23 06:00
AID - 10.1089/vim.2009.0079 [doi]
PST - ppublish
SO  - Viral Immunol. 2010 Jun;23(3):309-19. doi: 10.1089/vim.2009.0079.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>