Combined detections of interleukin 27, interferon-γ, and adenosine deaminase in pleural effusion for diagnosis of tuberculous pleurisy.
|Abstract||Previous studies reported interleukin-27 (IL-27), interferon-γ (IFN-γ), or adenosine deaminase (ADA) alone plays a helpful role in diagnosing tuberculous pleural effusion (TPE). The present study aims at comparing the diagnostic accuracy of pleural IL-27, IFN-γ, and ADA, and investigate the diagnostic accuracy of the combination of IL-27, IFN-γ, or/and ADA for differentiating TPE from pleural effusions with the other etiologies.|
Diagnostic accuracy of tumor necrosis factor-alpha, interferon-gamma, interleukin-10 and adenosine deaminase 2 in differential diagnosis between tuberculous pleural effusion and malignant pleural effusion.
|Journal Title||chinese medical journal|
|Publication Year Start||2014-01-01|
PMID- 24033939 OWN - NLM STAT- MEDLINE DA - 20130916 DCOM- 20140407 LR - 20130916 IS - 0366-6999 (Print) IS - 0366-6999 (Linking) VI - 126 IP - 17 DP - 2013 TI - Combined detections of interleukin 27, interferon-gamma, and adenosine deaminase in pleural effusion for diagnosis of tuberculous pleurisy. PG - 3215-21 AB - BACKGROUND: Previous studies reported interleukin-27 (IL-27), interferon-gamma (IFN-gamma), or adenosine deaminase (ADA) alone plays a helpful role in diagnosing tuberculous pleural effusion (TPE). The present study aims at comparing the diagnostic accuracy of pleural IL-27, IFN-gamma, and ADA, and investigate the diagnostic accuracy of the combination of IL-27, IFN-gamma, or/and ADA for differentiating TPE from pleural effusions with the other etiologies. METHODS: The concentrations of IL-27, IFN-gamma and ADA were simultaneously determined in pleural fluids and sera from 40 patients with TPE; 26 with malignant pleural effusion, seven with infectious pleural effusion, and eight with transudative pleural effusion by enzyme linked immunosorbent assay and colorimetric method. The corresponding biochemical indexs were also simultaneously determined. RESULTS: The concentrations of pleural IL-27 and IFN-gamma in the tuberculous group were significantly higher than those in the malignant, infectious, and transudative groups. The concentrations of ADA in TPE were significantly higher than those in MPE or transudative effusions, while much lower than those in infectious effusions. Among these three biomarkers, IL-27 was the most effective for TPE diagnosis, with the cut off value of 900.8 ng/L. IL-27 had a high sensitivity of 95% and specificity of 97.6% for differential diagnosis of TPE from non-TPEs. Combinations of IL-27, IFN-gamma and ADA measurements further increased the sensitivity or specificity up to 100%. CONCLUSIONS: Compared to non-TPEs, IL-27, IFN-gamma and ADA all simultaneously increased in TPE; and among these three rapid detection methods, IL-27 appeared to be the best for distinguishing tuberculous from non-TPEs, especially from MPE. Combinations of the three markers (IL-27, IFN-gamma and ADA) yielded the highest sensitivity and specificity. These findings suggest that the applications of a new biomarker, IL-27, alone or with IFN-gamma and ADA, may contribute to more efficient diagnosis strategies in the management of tuberculous pleurisy. FAU - Wu, Yan-Bin AU - Wu YB AD - Institute of Respiratory Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, China. FAU - Ye, Zhi-Jian AU - Ye ZJ FAU - Qin, Sou-Ming AU - Qin SM FAU - Wu, Cong AU - Wu C FAU - Chen, Yi-Qiang AU - Chen YQ FAU - Shi, Huan-Zhong AU - Shi HZ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Chin Med J (Engl) JT - Chinese medical journal JID - 7513795 RN - 0 (Interleukin-27) RN - 82115-62-6 (Interferon-gamma) RN - EC 220.127.116.11 (Adenosine Deaminase) SB - IM MH - Adenosine Deaminase/blood/*metabolism MH - Aged MH - Female MH - Humans MH - Interferon-gamma/blood/*metabolism MH - Interleukin-27/blood/*metabolism MH - Male MH - Middle Aged MH - Pleural Effusion/blood/*metabolism MH - Tuberculosis, Pleural/blood/*diagnosis/*metabolism EDAT- 2013/09/17 06:00 MHDA- 2014/04/08 06:00 CRDT- 2013/09/17 06:00 PST - ppublish SO - Chin Med J (Engl). 2013;126(17):3215-21.