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Immune Responses in Cecal Tonsils of Marek's Disease Virus-Infected Chickens.

Abstract Marek's disease (MD) is a lymphoproliferative disease of domestic chickens that is caused by a highly cell-associated oncogenic α-herpesvirus, Marek's disease virus (MDV). MDV replicates in chicken lymphocytes and establishes a latent infection within CD4+ T cells. Clinical signs of MD include depression, crippling, weight loss, bursal/thymic atrophy, neurologic disorders, and rapid onset of T cell lymphomas that infiltrate lymphoid tissues, visceral organs, and peripheral nerves. The cecal tonsils (CTs) are considered the largest lymphoid aggregates of avian gut-associated lymphoid tissue. Along with Peyer's patches, CTs elicit protective immune responses against bacterial and viral pathogens in the intestinal tract of avian species. In this study, we investigated the effect of MDV infection on toll-like receptor (TLR) gene expression in CTs of MD-susceptible (72) and resistant (63) chicken lines. Real-time PCR gene expression profiling revealed that of the 10 TLRs tested, TLR2A, TLR3, TLR5, and TLR15 displayed significant differential expression patterns at different time points postinoculation. The expression levels of the remaining six genes were minimally affected by MDV infection in either line. Immunohistochemical analysis showed a severe depletion of B cells and CD4+ T cells in the CTs of susceptible line at 5 days postinfection (dpi), which recovered by 21 dpi. The destruction of B and T cells in the CTs of the resistant line was minimal at 5 dpi, which also recovered by 21 dpi. A significant infiltration of macrophages was observed after the depletion of B and T cells in the infected birds of both lines that could account for the differential TLR gene expression in the infected birds. The data presented provide further insight into the mechanism of MDV pathogenesis and tissue-specific immunologic responses to viral infection.
PMID
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Authors

Mayor MeshTerms

Chickens

Keywords

Marek's disease

cecal tonsil

real-time PCR

toll-like receptors

Journal Title avian diseases
Publication Year Start




PMID- 26473671
OWN - NLM
STAT- MEDLINE
DA  - 20151019
DCOM- 20151217
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 59
IP  - 2
DP  - 2015 Jun
TI  - Immune Responses in Cecal Tonsils of Marek's Disease Virus-Infected Chickens.
PG  - 213-26
LID - 10.1637/10950-093014-Reg.1 [doi]
AB  - Marek's disease (MD) is a lymphoproliferative disease of domestic chickens that
      is caused by a highly cell-associated oncogenic alpha-herpesvirus, Marek's
      disease virus (MDV). MDV replicates in chicken lymphocytes and establishes a
      latent infection within CD4+ T cells. Clinical signs of MD include depression,
      crippling, weight loss, bursal/thymic atrophy, neurologic disorders, and rapid
      onset of T cell lymphomas that infiltrate lymphoid tissues, visceral organs, and 
      peripheral nerves. The cecal tonsils (CTs) are considered the largest lymphoid
      aggregates of avian gut-associated lymphoid tissue. Along with Peyer's patches,
      CTs elicit protective immune responses against bacterial and viral pathogens in
      the intestinal tract of avian species. In this study, we investigated the effect 
      of MDV infection on toll-like receptor (TLR) gene expression in CTs of
      MD-susceptible (72) and resistant (63) chicken lines. Real-time PCR gene
      expression profiling revealed that of the 10 TLRs tested, TLR2A, TLR3, TLR5, and 
      TLR15 displayed significant differential expression patterns at different time
      points postinoculation. The expression levels of the remaining six genes were
      minimally affected by MDV infection in either line. Immunohistochemical analysis 
      showed a severe depletion of B cells and CD4+ T cells in the CTs of susceptible
      line at 5 days postinfection (dpi), which recovered by 21 dpi. The destruction of
      B and T cells in the CTs of the resistant line was minimal at 5 dpi, which also
      recovered by 21 dpi. A significant infiltration of macrophages was observed after
      the depletion of B and T cells in the infected birds of both lines that could
      account for the differential TLR gene expression in the infected birds. The data 
      presented provide further insight into the mechanism of MDV pathogenesis and
      tissue-specific immunologic responses to viral infection.
FAU - Heidari, Mohammad
AU  - Heidari M
AD  - A United States Department of Agriculture, Agriculture Research Service, Avian
      Disease and Oncology Laboratory, East Lansing, MI 48823.
FAU - Fitzgerald, Scott D
AU  - Fitzgerald SD
AD  - B Pathobiology and Diagnostic Investigation, College of Veterinary Medicine,
      Michigan State University, East Lansing, MI 48824.
FAU - Zhang, Huanmin
AU  - Zhang H
AD  - A United States Department of Agriculture, Agriculture Research Service, Avian
      Disease and Oncology Laboratory, East Lansing, MI 48823.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
SB  - IM
MH  - Animals
MH  - Cecum/pathology
MH  - *Chickens
MH  - Lymphoid Tissue/*immunology/pathology
MH  - Marek Disease/*immunology/pathology
MH  - Random Allocation
MH  - Specific Pathogen-Free Organisms
OTO - NOTNLM
OT  - Marek's disease
OT  - cecal tonsil
OT  - real-time PCR
OT  - toll-like receptors
EDAT- 2015/10/17 06:00
MHDA- 2015/12/19 06:00
CRDT- 2015/10/17 06:00
AID - 10.1637/10950-093014-Reg.1 [doi]
PST - ppublish
SO  - Avian Dis. 2015 Jun;59(2):213-26. doi: 10.1637/10950-093014-Reg.1.

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