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A functional variant of miRNA-149 confers risk for allergic rhinitis and comorbid asthma in Chinese children.

Abstract The prevalence of allergic rhinitis (AR) and asthma has been increasing, and the comorbidity rates of these diseases are very high. Here, 176 AR patients, 124 patients with comorbid AR and asthma (AR-A) and 206 healthy Chinese children as controls were included in a case-control study. Six single-nucleotide polymorphisms (SNPs), miR-146a (rs2910164, rs57095329 and rs6864584), miR-196a2 (rs11614913), miR-499 (rs3746444) and miR-149 (rs2292832), were genotyped. The prevalence of homozygous miR-149 (rs2292832) CC genotype and C allele were considerably increased in AR and AR-A patients, compared with the controls. AR-A group showed higher frequencies of CC genotype and C allele of rs2292832 than AR group. No significant difference in the genotypic and allelic frequencies of other miRNA SNPs was found between the groups. MiR-149 levels in peripheral blood mononuclear cells (PBMCs) were significantly lower in CC (variant type) cases compared with TT (wild-type) cases. In further experiments, PBMCs obtained from the healthy controls with CC, CT and TT genotypes were stimulated by house dust mite extracts, which led to a significant decrease in the levels of miR-149 in PBMCs obtained from CC and TT individuals. This decrease was more pronounced in CC compared with TT cases. Our results demonstrate that miR-149 rs2292832 variant is not only strongly associated with AR and AR-A, but it may lead to an increase in the susceptibility to allergies following the stimulation with an allergen, through the changes in miR149 expression. Additionally, AR patients with CC genotypes were shown to be more susceptible to asthma.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title international journal of immunogenetics
Publication Year Start




PMID- 28181414
OWN - NLM
STAT- MEDLINE
DA  - 20170209
DCOM- 20170317
LR  - 20170317
IS  - 1744-313X (Electronic)
IS  - 1744-3121 (Linking)
VI  - 44
IP  - 2
DP  - 2017 Apr
TI  - A functional variant of miRNA-149 confers risk for allergic rhinitis and comorbid
      asthma in Chinese children.
PG  - 62-70
LID - 10.1111/iji.12307 [doi]
AB  - The prevalence of allergic rhinitis (AR) and asthma has been increasing, and the 
      comorbidity rates of these diseases are very high. Here, 176 AR patients, 124
      patients with comorbid AR and asthma (AR-A) and 206 healthy Chinese children as
      controls were included in a case-control study. Six single-nucleotide
      polymorphisms (SNPs), miR-146a (rs2910164, rs57095329 and rs6864584), miR-196a2
      (rs11614913), miR-499 (rs3746444) and miR-149 (rs2292832), were genotyped. The
      prevalence of homozygous miR-149 (rs2292832) CC genotype and C allele were
      considerably increased in AR and AR-A patients, compared with the controls. AR-A 
      group showed higher frequencies of CC genotype and C allele of rs2292832 than AR 
      group. No significant difference in the genotypic and allelic frequencies of
      other miRNA SNPs was found between the groups. MiR-149 levels in peripheral blood
      mononuclear cells (PBMCs) were significantly lower in CC (variant type) cases
      compared with TT (wild-type) cases. In further experiments, PBMCs obtained from
      the healthy controls with CC, CT and TT genotypes were stimulated by house dust
      mite extracts, which led to a significant decrease in the levels of miR-149 in
      PBMCs obtained from CC and TT individuals. This decrease was more pronounced in
      CC compared with TT cases. Our results demonstrate that miR-149 rs2292832 variant
      is not only strongly associated with AR and AR-A, but it may lead to an increase 
      in the susceptibility to allergies following the stimulation with an allergen,
      through the changes in miR149 expression. Additionally, AR patients with CC
      genotypes were shown to be more susceptible to asthma.
CI  - (c) 2017 John Wiley & Sons Ltd.
FAU - Hu, D
AU  - Hu D
AD  - Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing
      Medical University, Chongqing, China.
FAU - Zhang, Z
AU  - Zhang Z
AD  - Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing
      Medical University, Chongqing, China.
FAU - Ke, X
AU  - Ke X
AD  - Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing
      Medical University, Chongqing, China.
FAU - Kang, H
AU  - Kang H
AD  - Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing
      Medical University, Chongqing, China.
FAU - Hong, S
AU  - Hong S
AUID- ORCID: http://orcid.org/0000-0003-0627-1488
AD  - Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing
      Medical University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20170208
PL  - England
TA  - Int J Immunogenet
JT  - International journal of immunogenetics
JID - 101232337
RN  - 0 (MIRN149 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - Animals
MH  - Asthma/complications/*genetics/pathology
MH  - Child
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - MicroRNAs/biosynthesis/*genetics
MH  - Polymorphism, Single Nucleotide
MH  - Pyroglyphidae/genetics/pathogenicity
MH  - Rhinitis, Allergic/complications/*genetics/immunology/pathology
MH  - Risk Factors
EDAT- 2017/02/10 06:00
MHDA- 2017/03/18 06:00
CRDT- 2017/02/10 06:00
PHST- 2016/03/31 [received]
PHST- 2016/12/15 [revised]
PHST- 2017/01/14 [accepted]
AID - 10.1111/iji.12307 [doi]
PST - ppublish
SO  - Int J Immunogenet. 2017 Apr;44(2):62-70. doi: 10.1111/iji.12307. Epub 2017 Feb 8.

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