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Stryphnodendron adstringens and purified tannin on Pythium insidiosum: in vitro and in vivo studies.

Abstract Pythium insidiosum is the etiological agent of pythiosis, an emerging life-threatening infectious disease in tropical and subtropical regions. The pathogen is a fungus-like organism resistant to antifungal therapy, for this reason, most cases need extensive surgical debridments as treatment, but depending on the size and anatomical region of the lesion, such approach is unfeasible. We investigate the fungicidal effect and toxicity of crude bark extract of Stryphnodendron adstringens and commercially available tannin on Pythium insidiosum both in vitro and in vivo.
PMID
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Authors

Mayor MeshTerms
Keywords

Oomycete

Pythiosis

Pythium insidiosum

Susceptibility tests

Journal Title annals of clinical microbiology and antimicrobials
Publication Year Start




PMID- 28231795
OWN - NLM
STAT- MEDLINE
DA  - 20170224
DCOM- 20170303
LR  - 20170303
IS  - 1476-0711 (Electronic)
IS  - 1476-0711 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Feb 23
TI  - Stryphnodendron adstringens and purified tannin on Pythium insidiosum: in vitro
      and in vivo studies.
PG  - 7
LID - 10.1186/s12941-017-0183-3 [doi]
AB  - BACKGROUND: Pythium insidiosum is the etiological agent of pythiosis, an emerging
      life-threatening infectious disease in tropical and subtropical regions. The
      pathogen is a fungus-like organism resistant to antifungal therapy, for this
      reason, most cases need extensive surgical debridments as treatment, but
      depending on the size and anatomical region of the lesion, such approach is
      unfeasible. We investigate the fungicidal effect and toxicity of crude bark
      extract of Stryphnodendron adstringens and commercially available tannin on
      Pythium insidiosum both in vitro and in vivo. METHODS: Standardized fragments of 
      mycelia of fifteen isolates of P. insidiosum were tested with different
      concentrations of bark extract (10 to 30% v/v) and tannin (0.5, 1.0 and 1.5
      mg/mL). For in vivo study, fifteen rabbits were experimentally infected with
      zoospores of P. insidiosum and treated by oral and intralesional applications of 
      bark extract and tannin. Acute toxicity tests with both substances were also
      performed in rats. RESULTS: In vitro studies showed fungicidal effect for both
      substances at different concentrations and the SEM showed alteration on the cell 
      wall surface of the pathogen. All infected rabbits developed a firm nodular mass 
      that reached around 90 mm2 ninety days after inoculation, but neither the
      intralesional inoculation of tannin, nor the oral administration of crude extract
      and tannin were able to promote remission of the lesions. CONCLUSIONS: Lesions
      developed by rabbits presented an encapsulated abscess being quite different of
      naturally acquired pythiosis, which is characterized by ulcerated lesions. Since 
      no toxicity was observed in rats or rabbits inoculated with these products, while
      in vitro experiments showed direct antifungal effect, therapeutic activity of S. 
      adstringens and tannin should be clinically tested as an alternative for healing 
      wounds in naturally acquired pythiosis.
FAU - Trolezi, Rodrigo
AU  - Trolezi R
AD  - Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, 
      UNESP Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
FAU - Azanha, Juliana Maziero
AU  - Azanha JM
AD  - Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, 
      UNESP Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
FAU - Paschoal, Natalia Rodrigues
AU  - Paschoal NR
AD  - School of Veterinary Medicine and Animal Science, UNESP Univ Estadual Paulista,
      Botucatu, SP, Brazil.
FAU - Chechi, Jessica Luana
AU  - Chechi JL
AD  - Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, 
      UNESP Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
FAU - Dias Silva, Marcelo Jose
AU  - Dias Silva MJ
AD  - Institute of Chemistry, UNESP Univ Estadual Paulista, Araraquara, SP, Brazil.
FAU - Fabris, Viciany Eric
AU  - Fabris VE
AD  - Department of Pathology, Botucatu School of Medicine, UNESP Univ Estadual
      Paulista, Botucatu, SP, Brazil.
FAU - Vilegas, Wagner
AU  - Vilegas W
AD  - Institute of Chemistry, UNESP Univ Estadual Paulista, Araraquara, SP, Brazil.
FAU - Kaneno, Ramon
AU  - Kaneno R
AD  - Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, 
      UNESP Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
FAU - Fernandes Junior, Ary
AU  - Fernandes Junior A
AD  - Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, 
      UNESP Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
FAU - Bosco, Sandra de Moraes Gimenes
AU  - Bosco SM
AD  - Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, 
      UNESP Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
      [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170223
PL  - England
TA  - Ann Clin Microbiol Antimicrob
JT  - Annals of clinical microbiology and antimicrobials
JID - 101152152
RN  - 0 (Antifungal Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Tannins)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antifungal Agents/*pharmacology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Fabaceae/*chemistry
MH  - Humans
MH  - Injections, Intralesional
MH  - Male
MH  - Mycelium/*drug effects/growth & development/ultrastructure
MH  - Plant Bark/chemistry
MH  - Plant Extracts/chemistry
MH  - Pythiosis/*drug therapy/microbiology/pathology
MH  - Pythium/*drug effects/growth & development/ultrastructure
MH  - Rabbits
MH  - Rats
MH  - Rats, Wistar
MH  - Tannins/*pharmacology
PMC - PMC5324290
OTO - NOTNLM
OT  - Oomycete
OT  - Pythiosis
OT  - Pythium insidiosum
OT  - Susceptibility tests
EDAT- 2017/02/25 06:00
MHDA- 2017/03/04 06:00
CRDT- 2017/02/25 06:00
PHST- 2016/06/29 [received]
PHST- 2017/02/09 [accepted]
AID - 10.1186/s12941-017-0183-3 [doi]
AID - 10.1186/s12941-017-0183-3 [pii]
PST - epublish
SO  - Ann Clin Microbiol Antimicrob. 2017 Feb 23;16(1):7. doi:
      10.1186/s12941-017-0183-3.

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