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Miniature Dielectric Barrier Discharge Nonthermal Plasma Induces Apoptosis in Lung Cancer Cells and Inhibits Cell Migration.

Abstract Traditional cancer treatments like radiotherapy and chemotherapy have drawbacks and are not selective for killing only cancer cells. Nonthermal atmospheric pressure plasmas with dielectric barrier discharge (DBD) can be applied to living cells and tissues and have emerged as novel tools for localized cancer therapy. The purpose of this study was to investigate the different effects caused by miniature DBD (mDBD) plasma to A549 lung cancer cells. In this study, A549 lung cancer cells cultured in 12 well plates were treated with mDBD plasma for specified treatment times to assess the changes in the size of the area of cell detachment, the viability of attached or detached cells, and cell migration. Furthermore, we investigated an innovative mDBD plasma-based therapy for localized treatment of lung cancer cells through apoptotic induction. Our results indicate that plasma treatment for 120 sec causes apoptotic cell death in 35.8% of cells, while mDBD plasma treatment for 60 sec, 30 sec, or 15 sec causes apoptotic cell death in 20.5%, 14.1%, and 6.3% of the cell population, respectively. Additionally, we observed reduced A549 cell migration in response to mDBD plasma treatment. Thus, mDBD plasma system can be a viable platform for localized lung cancer therapy.
PMID
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Authors

Mayor MeshTerms

Electricity

Keywords
Journal Title biomed research international
Publication Year Start




PMID- 28243603
OWN - NLM
STAT- MEDLINE
DA  - 20170228
DCOM- 20170313
LR  - 20170313
IS  - 2314-6141 (Electronic)
VI  - 2017
DP  - 2017
TI  - Miniature Dielectric Barrier Discharge Nonthermal Plasma Induces Apoptosis in
      Lung Cancer Cells and Inhibits Cell Migration.
PG  - 8058307
LID - 10.1155/2017/8058307 [doi]
AB  - Traditional cancer treatments like radiotherapy and chemotherapy have drawbacks
      and are not selective for killing only cancer cells. Nonthermal atmospheric
      pressure plasmas with dielectric barrier discharge (DBD) can be applied to living
      cells and tissues and have emerged as novel tools for localized cancer therapy.
      The purpose of this study was to investigate the different effects caused by
      miniature DBD (mDBD) plasma to A549 lung cancer cells. In this study, A549 lung
      cancer cells cultured in 12 well plates were treated with mDBD plasma for
      specified treatment times to assess the changes in the size of the area of cell
      detachment, the viability of attached or detached cells, and cell migration.
      Furthermore, we investigated an innovative mDBD plasma-based therapy for
      localized treatment of lung cancer cells through apoptotic induction. Our results
      indicate that plasma treatment for 120 sec causes apoptotic cell death in 35.8%
      of cells, while mDBD plasma treatment for 60 sec, 30 sec, or 15 sec causes
      apoptotic cell death in 20.5%, 14.1%, and 6.3% of the cell population,
      respectively. Additionally, we observed reduced A549 cell migration in response
      to mDBD plasma treatment. Thus, mDBD plasma system can be a viable platform for
      localized lung cancer therapy.
FAU - Karki, Surya B
AU  - Karki SB
AUID- ORCID: 0000-0002-8595-3219
AD  - Department of Bioengineering, College of Engineering, University of Toledo,
      Toledo, OH 43606, USA.
FAU - Yildirim-Ayan, Eda
AU  - Yildirim-Ayan E
AUID- ORCID: 0000-0003-3275-167X
AD  - Department of Bioengineering, College of Engineering, University of Toledo,
      Toledo, OH 43606, USA; Department of Orthopaedic Surgery, University of Toledo
      Medical Center, Toledo, OH 43614, USA.
FAU - Eisenmann, Kathryn M
AU  - Eisenmann KM
AD  - Department of Biochemistry and Cancer Biology, University of Toledo Health
      Science Campus, Toledo, OH 43614, USA.
FAU - Ayan, Halim
AU  - Ayan H
AUID- ORCID: 0000-0003-4103-9772
AD  - Department of Bioengineering, College of Engineering, University of Toledo,
      Toledo, OH 43606, USA; Department of Mechanical, Industrial, and Manufacturing
      Engineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA.
LA  - eng
PT  - Journal Article
DEP - 20170124
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Plasma Gases)
RN  - Adenocarcinoma of lung
SB  - IM
MH  - A549 Cells
MH  - Adenocarcinoma/pathology
MH  - Apoptosis/*drug effects
MH  - Cell Count
MH  - Cell Movement/*drug effects
MH  - Cell Survival/drug effects
MH  - *Electricity
MH  - Epithelial Cells/drug effects/pathology
MH  - Humans
MH  - Lung Neoplasms/*pathology
MH  - Microscopy, Fluorescence
MH  - Plasma Gases/*pharmacology
MH  - Time Factors
PMC - PMC5294360
COI - The authors declare that there is no conflict of interests regarding the
      publication of this paper.
EDAT- 2017/03/01 06:00
MHDA- 2017/03/14 06:00
CRDT- 2017/03/01 06:00
PHST- 2016/07/26 [received]
PHST- 2016/11/22 [revised]
PHST- 2016/12/19 [accepted]
AID - 10.1155/2017/8058307 [doi]
PST - ppublish
SO  - Biomed Res Int. 2017;2017:8058307. doi: 10.1155/2017/8058307. Epub 2017 Jan 24.

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