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Cryptosporidium species and subtypes in diarrheal children and HIV-infected persons in Ebonyi and Nsukka, Nigeria.

Abstract Cryptosporidiosis is a common disease of children and immune-compromised persons. This study evaluated the diversity and distribution of Cryptosporidium species in diarrheal children and HIV-infected persons on highly active antiretroviral therapy (HAART) and those not on HAART.
PMID
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Authors

Mayor MeshTerms

Genetic Variation

Genotype

Keywords
Journal Title journal of infection in developing countries
Publication Year Start




PMID- 28248679
OWN - NLM
STAT- MEDLINE
DA  - 20170301
DCOM- 20170315
LR  - 20170315
IS  - 1972-2680 (Electronic)
IS  - 1972-2680 (Linking)
VI  - 11
IP  - 2
DP  - 2017 Feb 28
TI  - Cryptosporidium species and subtypes in diarrheal children and HIV-infected
      persons in Ebonyi and Nsukka, Nigeria.
PG  - 173-179
LID - 10.3855/jidc.8034 [doi]
AB  - INTRODUCTION: Cryptosporidiosis is a common disease of children and
      immune-compromised persons. This study evaluated the diversity and distribution
      of Cryptosporidium species in diarrheal children and HIV-infected persons on
      highly active antiretroviral therapy (HAART) and those not on HAART. METHODOLOGY:
      A total of 394 fecal specimens were collected from patients attending clinics in 
      Nsukka and Ebonyi, Nigeria. Detection and identification of Cryptosporidium
      species were conducted by PCR-RFLP of the small subunit (SSU) rRNA gene, whereas 
      subtyping was done by sequence analysis of the 60 kDa glycoprotein (gp60) gene.
      RESULTS: Twenty-five (6.3%) specimens yielded four Cryptosporidium species,
      including C. hominis, C. parvum, C. felis, and C. viatorum. C. hominis was the
      most dominant species with 48.0% occurrence and three identified subtype
      families: Ia (six specimens), Ib (three specimens), Ie (two specimens), and one
      un-subtyped species. C. parvum had 44.0% occurrence and two subtype families: IIc
      (eight specimens) and IIe (three specimens), while C. felis and C. viatorum each 
      had 4.0% occurrence. There were significant differences in Cryptosporidium
      species distribution between age groups in children and HIV-infected persons,
      between suburban and urban areas, and between low and high CD4+ cell counts in
      HIV-infected patients. There were no significant differences in infection rate
      and species distribution between HIV-infected patients on HAART and those not on 
      HAART. CONCLUSIONS: The results from this study show that there is a high
      diversity of Cryptosporidium spp. in humans in Ebonyi and Nsukka, Nigeria, and
      that all the C. parvum subtypes identified are most likely anthroponotic in
      origin.
FAU - Ukwah, Boniface Nwofoke
AU  - Ukwah BN
AD  - Faculty of Health Sciences, Ebonyi State University, Abakaliki, Nigeria.
      [email protected]
FAU - Ezeonu, Ifeoma Maureen
AU  - Ezeonu IM
FAU - Ezeonu, Chinonyelum Thecla
AU  - Ezeonu CT
FAU - Roellig, Dawn
AU  - Roellig D
FAU - Xiao, Lihua
AU  - Xiao L
LA  - eng
PT  - Journal Article
DEP - 20170228
PL  - Italy
TA  - J Infect Dev Ctries
JT  - Journal of infection in developing countries
JID - 101305410
RN  - 0 (Glycoproteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Cryptosporidiosis/epidemiology/*parasitology
MH  - Cryptosporidium/*classification/*genetics/isolation & purification
MH  - DNA Fingerprinting
MH  - Diarrhea/epidemiology/*parasitology
MH  - Feces/parasitology
MH  - Female
MH  - *Genetic Variation
MH  - *Genotype
MH  - Glycoproteins/genetics
MH  - HIV Infections/*complications
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Molecular Epidemiology
MH  - Nigeria/epidemiology
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Protozoan Proteins/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - Young Adult
EDAT- 2017/03/02 06:00
MHDA- 2017/03/16 06:00
CRDT- 2017/03/02 06:00
PHST- 2015/12/22 [received]
PHST- 2016/02/23 [accepted]
PHST- 2016/03/09 [revised]
PST - epublish
SO  - J Infect Dev Ctries. 2017 Feb 28;11(2):173-179. doi: 10.3855/jidc.8034.

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