OWN - NLM
DA - 20170301
LR - 20170301
IS - 1439-3646 (Electronic)
IS - 0947-7349 (Linking)
VI - 125
IP - 2
DP - 2017 Feb
TI - Pancreas Transplantation Delays the Progression of Morphological, Morphometric
and Ultrastructural Changes in Testes of Alloxan-Induced Diabetic Rats.
PG - 106-115
LID - 10.1055/s-0042-122137 [doi]
AB - Aim: The aim of this study was to assess the effects of pancreas transplantation
on the progression of testicular lesions in diabetic rats. Methods: Diabetic rats
were subjected to pancreas transplantation and sacrificed after 6, 14, 26 and 50
weeks of follow-up, using non-diabetic and untreated diabetic rats as controls.
Results: Successful pancreas transplantation corrected all of the metabolic
changes observed in diabetic rats, including low levels of testosterone. The
testicular mass was decreased, and the relative weight of the testes was high in
diabetic rats. The seminiferous tubules of diabetic rats showed progressive
atrophy of the germinal epithelium, with cytoplasmic vacuolization, detachment of
germ cells to the tubular lumen and the appearance of giant cells. Leydig cells
were abnormally distributed, and hyperplasia of Sertoli cells was observed. Sperm
were not detectable within the tubular lumen in late follow-up. The diameter,
total area, lumen area, and germinal epithelium area of the seminiferous tubules
were low, and tubular density was high in diabetic rats. Ultrastructural changes
were also observed in these rats, compromising the cytoplasm, organelles and
cellular nuclei of the germ, Sertoli, and Leydig cells. The most frequent changes
consisted of accumulation of lipid droplets and electron-dense dark material in
the cell cytoplasm, cellular degeneration and apoptosis. Similar to non-diabetic
rats, pancreas-transplanted rats showed progressive testicular lesions, but they
were much less severe and occurred much later than in the untreated diabetic
controls. Conclusion: Diabetes causes morphological and ultrastructural changes
in rat testes, but the progression of lesions can be significantly delayed by
successful pancreas transplantation, which may have a positive impact on male
infertility due to diabetes.
CI - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Tadeu Spadella, Cesar
AU - Tadeu Spadella C
AD - Surgery and Orthopedics, Botucatu School of Medicine - UNESP, Botucatu, Brazil.
FAU - Teixeira Trindade, Amelia Arcangela
AU - Teixeira Trindade AA
AD - Medicine Course, Federal University of Sao Carlos - UFSCAR, Medicine, Sao Carlos,
FAU - Natalia Lucchesi, Amanda
AU - Natalia Lucchesi A
AD - Faculty of Medicine of Botucatu, UNESP, Graduate Program in General Basis of
Surgery, Botucatu, Brazil.
FAU - Sperandeo de Macedo, Celia
AU - Sperandeo de Macedo C
AD - Faculty of Medicine of Botucatu, UNESP, Pediatrics, Botucatu, Brazil.
LA - eng
PT - Journal Article
DEP - 20170301
PL - Germany
TA - Exp Clin Endocrinol Diabetes
JT - Experimental and clinical endocrinology & diabetes : official journal, German
Society of Endocrinology [and] German Diabetes Association
JID - 9505926
EDAT- 2017/03/02 06:00
MHDA- 2017/03/02 06:00
CRDT- 2017/03/02 06:00
AID - 10.1055/s-0042-122137 [doi]
PST - ppublish
SO - Exp Clin Endocrinol Diabetes. 2017 Feb;125(2):106-115. doi:
10.1055/s-0042-122137. Epub 2017 Mar 1.