PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 28249915
OWN - NLM
STAT- MEDLINE
DA  - 20170302
DCOM- 20170313
LR  - 20170313
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 47
IP  - 1
DP  - 2017 Jan
TI  - Cobalt Chloride-induced Hypoxia Induces Epithelial-mesenchymal Transition in
      Renal Carcinoma Cell Lines.
PG  - 40-46
AB  - OBJECTIVE: To establish epithelial-mesenchymal transition (EMT) models in renal
      cell carcinoma (RCC) cell lines. MATERIALS AND METHODS: The RCC cell lines A498
      and 786-O were used in the experiment and CoCl2 was used to simulate hypoxia.
      Cells were cultured with different concentrations of CoCl2. Morphology and
      changes in cytoactivity were observed. After CoCl2 treatment, the expression of
      HIF-1alpha and the changes of EMT-related molecules (E-cadherin, fibronectin)
      were detected. RESULTS: Cell conjunctions of CoCl2-treated groups were loose and 
      scattered compared to the control. CoCl2 did not promote or attenuate the
      viability of A498 cells at low dosage, but when the concentration of CoCl2
      reached 250 muM, cell activity gradually declined. In contrast, CoCl2 induced
      786-O cell proliferation in the range of 50 mu M-200 mu M, but inhibited cell
      growth at dosages higher than 200 muM. The expression of E-cadherin was
      significantly down-regulated, and fibronectin was up-regulated in both A498 and
      786-O cell lines under CoCl2-simulated hypoxia in comparison with normoxic
      conditions (P<0.01). CONCLUSIONS: CoCl2-induced hypoxia could induce EMT in RCC
      cell lines. The models will help us further study the mechanisms of EMT and
      investigate novel therapeutic targets to inhibit tumor invasion and metastasis.
CI  - (c) 2017 by the Association of Clinical Scientists, Inc.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Department of Urology, Peking University Cancer Hospital, Beijing Institute for
      Cancer Research, Beijing, People's Republic of China.
FAU - Hong, Baoan
AU  - Hong B
AD  - Department of Urology, Peking University First Hospital, Beijing, People's
      Republic of China.
FAU - Zhou, Changhua
AU  - Zhou C
AD  - School of Pharmaceutical Sciences, Center for Cellular and Structural Biology,
      Sun Yat-Sen University, Guangzhou, People's Republic of China.
FAU - Du, Xin
AU  - Du X
AD  - Department of Urology, Beijing Chaoyang Hospital, Capital Medical University,
      Beijing, People's Republic of China.
FAU - Chen, Siqi
AU  - Chen S
AD  - School of Pharmaceutical Sciences, Center for Cellular and Structural Biology,
      Sun Yat-Sen University, Guangzhou, People's Republic of China.
FAU - Deng, Xiaohu
AU  - Deng X
AD  - Department of Urology, Karamay People's Hospital, Xinjiang, People's Republic of 
      China.
FAU - Duoerkun, Shayiremu
AU  - Duoerkun S
AD  - Department of Urology, Hami District Central Hospital, Xinjiang, People's
      Republic of China.
FAU - Li, Qing
AU  - Li Q
AD  - School of Pharmaceutical Sciences, Center for Cellular and Structural Biology,
      Sun Yat-Sen University, Guangzhou, People's Republic of China.
FAU - Yang, Yong
AU  - Yang Y
AD  - Department of Urology, Peking University Cancer Hospital, Beijing Institute for
      Cancer Research, Beijing, People's Republic of China [email protected]
FAU - Gong, Kan
AU  - Gong K
AD  - Department of Urology, Peking University First Hospital, Beijing, People's
      Republic of China [email protected]
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 3G0H8C9362 (Cobalt)
RN  - EVS87XF13W (cobaltous chloride)
SB  - IM
MH  - Carcinoma, Renal Cell/metabolism/*pathology
MH  - Cell Hypoxia/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Cobalt/*pharmacology
MH  - Epithelial-Mesenchymal Transition/*drug effects
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Kidney Neoplasms/metabolism/*pathology
OTO - NOTNLM
OT  - E-cadherin
OT  - Epithelial-mesenchymal transition
OT  - Fibronectin
OT  - Hypoxia
OT  - Renal cell carcinoma
EDAT- 2017/03/03 06:00
MHDA- 2017/03/14 06:00
CRDT- 2017/03/03 06:00
AID - 47/1/40 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2017 Jan;47(1):40-46.

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