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Inhibition of invasion and migration of prostate cancer cells by miRNA-509-5p via targeting MDM2.

Abstract Prostate cancer is a common malignancy of the male reproductive-urinary system. MDM2 is an oncogene, whose expression can be regulated by microRNA (miRNA). The present study investigated the expression and correlation of miRNA-509-5p and MDM2 to determine the mechanism of their function in invasion and migration of prostate cancer cells. RT-PCR was performed to detect the expression of miRNA-509-5p and MDM2 in tumor, tumor-adjacent, and normal tissues, obtained from prostate cancer patients, using the HGC-27 cell line as an in vitro model. Cultured HGC-27 cells were transfected with miRNA-509-5p mimics, miRNA-509-5p inhibitor, and mimic control. Expression levels of miRNA-509-5p and MDM2 were quantified by RT-PCR. Cell proliferation and invasion/migration were examined by the MTT and transwell assays, respectively. MiRNA-509-5p was significantly down-regulated in prostate cancer cells exhibiting high MDM2 mRNA levels. MiRNA mimic transfection elevated miRNA levels and suppressed MDM2 expression. With prolonged incubation time, the proliferation ratio and OD values of miRNA-509-5p mimic transfected cells decreased, along with decrease in cell migration and invasion. These results suggested that miRNA-509-5p negatively regulates MDM2 expression via targeting the 3'-UTR of genes. As a novel tumor suppressor, miRNA-509-5p in prostate cancer HGC-27 cells can suppress MDM2 expression and inhibit cell proliferation, invasion, and migration. Therefore, miRNA-509-5p could be used as a novel therapeutic agent in the treatment of prostate cancer.
PMID
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Authors

Mayor MeshTerms

Gene Expression Regulation, Neoplastic

Keywords
Journal Title genetics and molecular research : gmr
Publication Year Start




PMID- 28252164
OWN - NLM
STAT- MEDLINE
DA  - 20170302
DCOM- 20170317
LR  - 20170317
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Feb 23
TI  - Inhibition of invasion and migration of prostate cancer cells by miRNA-509-5p via
      targeting MDM2.
LID - 10.4238/gmr16019195 [doi]
AB  - Prostate cancer is a common malignancy of the male reproductive-urinary system.
      MDM2 is an oncogene, whose expression can be regulated by microRNA (miRNA). The
      present study investigated the expression and correlation of miRNA-509-5p and
      MDM2 to determine the mechanism of their function in invasion and migration of
      prostate cancer cells. RT-PCR was performed to detect the expression of
      miRNA-509-5p and MDM2 in tumor, tumor-adjacent, and normal tissues, obtained from
      prostate cancer patients, using the HGC-27 cell line as an in vitro model.
      Cultured HGC-27 cells were transfected with miRNA-509-5p mimics, miRNA-509-5p
      inhibitor, and mimic control. Expression levels of miRNA-509-5p and MDM2 were
      quantified by RT-PCR. Cell proliferation and invasion/migration were examined by 
      the MTT and transwell assays, respectively. MiRNA-509-5p was significantly
      down-regulated in prostate cancer cells exhibiting high MDM2 mRNA levels. MiRNA
      mimic transfection elevated miRNA levels and suppressed MDM2 expression. With
      prolonged incubation time, the proliferation ratio and OD values of miRNA-509-5p 
      mimic transfected cells decreased, along with decrease in cell migration and
      invasion. These results suggested that miRNA-509-5p negatively regulates MDM2
      expression via targeting the 3'-UTR of genes. As a novel tumor suppressor,
      miRNA-509-5p in prostate cancer HGC-27 cells can suppress MDM2 expression and
      inhibit cell proliferation, invasion, and migration. Therefore, miRNA-509-5p
      could be used as a novel therapeutic agent in the treatment of prostate cancer.
FAU - Tian, X M
AU  - Tian XM
AD  - Operation Room, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, Sichuan, China.
FAU - Luo, Y Z
AU  - Luo YZ
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, Sichuan, China.
FAU - He, P
AU  - He P
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, Sichuan, China.
FAU - Li, J
AU  - Li J
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, Sichuan, China.
FAU - Ma, Z W
AU  - Ma ZW
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, Sichuan, China.
FAU - An, Y
AU  - An Y
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, Sichuan, China [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170223
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN509 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Adult
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Proto-Oncogene Proteins c-mdm2/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Young Adult
EDAT- 2017/03/03 06:00
MHDA- 2017/03/18 06:00
CRDT- 2017/03/03 06:00
AID - gmr-16-01-gmr.16019195 [pii]
AID - 10.4238/gmr16019195 [doi]
PST - epublish
SO  - Genet Mol Res. 2017 Feb 23;16(1). doi: 10.4238/gmr16019195.

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