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Non-Typeable Haemophilus influenzae Infection of the Junbo Mouse.

Abstract Acute otitis media, inflammation of the middle ear bulla, is the most common bacterial infection in children. For one of the principal otopathogens, non-typeable Haemophilus influenzae (NTHi), animal models allow us to investigate host-microbial interactions relevant to the onset and progression of infection and to study treatment of middle ear disease. We have established a robust model of NTHi middle ear infection in the Junbo mouse. Intranasal inoculation with NTHi produces high rates of bulla infection and high bacterial titers in bulla fluids; bacteria can also spread down the respiratory tract to the mouse lung. An innate immune response is detected in the bulla of Junbo mice following NTHi infection, and bacteria are maintained in some ears at least up to day 56 post-inoculation. The Junbo/NTHi infection model facilitates studies on bacterial pathogenesis and antimicrobial intervention regimens and vaccines for better treatment and prevention of NTHi middle ear infection. © 2017 by John Wiley & Sons, Inc.
PMID
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A new model for non-typeable Haemophilus influenzae middle ear infection in the Junbo mutant mouse.

Authors

Mayor MeshTerms

Disease Models, Animal

Keywords

Junbo mouse

lung infection

middle ear infection

non-typeable Haemophilus influenzae (NTHi)

otitis media

Journal Title current protocols in mouse biology
Publication Year Start




PMID- 28252201
OWN - NLM
STAT- MEDLINE
DA  - 20170302
DCOM- 20170317
LR  - 20170317
IS  - 2161-2617 (Electronic)
IS  - 2161-2617 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Mar 02
TI  - Non-Typeable Haemophilus influenzae Infection of the Junbo Mouse.
PG  - 29-46
LID - 10.1002/cpmo.24 [doi]
AB  - Acute otitis media, inflammation of the middle ear bulla, is the most common
      bacterial infection in children. For one of the principal otopathogens,
      non-typeable Haemophilus influenzae (NTHi), animal models allow us to investigate
      host-microbial interactions relevant to the onset and progression of infection
      and to study treatment of middle ear disease. We have established a robust model 
      of NTHi middle ear infection in the Junbo mouse. Intranasal inoculation with NTHi
      produces high rates of bulla infection and high bacterial titers in bulla fluids;
      bacteria can also spread down the respiratory tract to the mouse lung. An innate 
      immune response is detected in the bulla of Junbo mice following NTHi infection, 
      and bacteria are maintained in some ears at least up to day 56 post-inoculation. 
      The Junbo/NTHi infection model facilitates studies on bacterial pathogenesis and 
      antimicrobial intervention regimens and vaccines for better treatment and
      prevention of NTHi middle ear infection. (c) 2017 by John Wiley & Sons, Inc.
CI  - Copyright (c) 2017 John Wiley & Sons, Inc.
FAU - Cheeseman, Michael T
AU  - Cheeseman MT
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of
      Veterinary Studies University of Edinburgh, Easter Bush, United Kingdom.
FAU - Hood, Derek W
AU  - Hood DW
AD  - Molecular Genetics Unit, MRC Harwell Institute, Harwell Science and Innovation
      Campus, Oxfordshire, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20170302
PL  - United States
TA  - Curr Protoc Mouse Biol
JT  - Current protocols in mouse biology
JID - 101560384
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Evi1 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/genetics
MH  - *Disease Models, Animal
MH  - Ear, Middle/metabolism/*microbiology/pathology
MH  - Haemophilus Infections/genetics/*microbiology
MH  - Haemophilus influenzae/*physiology
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Mice, Mutant Strains
MH  - Mutation, Missense
MH  - Otitis Media/genetics/*microbiology
MH  - Proto-Oncogenes/genetics
MH  - Pulmonary Infarction/genetics/microbiology
MH  - Transcription Factors/genetics
OTO - NOTNLM
OT  - Junbo mouse
OT  - lung infection
OT  - middle ear infection
OT  - non-typeable Haemophilus influenzae (NTHi)
OT  - otitis media
EDAT- 2017/03/03 06:00
MHDA- 2017/03/18 06:00
CRDT- 2017/03/03 06:00
AID - 10.1002/cpmo.24 [doi]
PST - epublish
SO  - Curr Protoc Mouse Biol. 2017 Mar 2;7(1):29-46. doi: 10.1002/cpmo.24.

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