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Brucella melitensis VirB12 recombinant protein is a potential marker for serodiagnosis of human brucellosis.

Abstract The numerous drawbacks of current serological tests for diagnosis of brucellosis which mainly results from cross reactivity with LPS from other gram-negative bacteria have generated an increasing interest to find more specific non-LPS antigens. Previous studies had indicated that Brucella VirB12 protein, a cell surface protein and component of type IV secretion system, induces antibody response during animal infection. However, this protein has not yet been tested as a serological diagnostic marker in human brucellosis.
PMID
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Authors

Mayor MeshTerms
Keywords

Brucella melitensis

Brucellosis

Enzyme-linked immunosorbent assay

Recombinant proteins

VirB12

Journal Title annals of clinical microbiology and antimicrobials
Publication Year Start




PMID- 28253917
OWN - NLM
STAT- MEDLINE
DA  - 20170303
DCOM- 20170307
LR  - 20170308
IS  - 1476-0711 (Electronic)
IS  - 1476-0711 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Mar 03
TI  - Brucella melitensis VirB12 recombinant protein is a potential marker for
      serodiagnosis of human brucellosis.
PG  - 8
LID - 10.1186/s12941-017-0182-4 [doi]
AB  - BACKGROUND: The numerous drawbacks of current serological tests for diagnosis of 
      brucellosis which mainly results from cross reactivity with LPS from other
      gram-negative bacteria have generated an increasing interest to find more
      specific non-LPS antigens. Previous studies had indicated that Brucella VirB12
      protein, a cell surface protein and component of type IV secretion system,
      induces antibody response during animal infection. However, this protein has not 
      yet been tested as a serological diagnostic marker in human brucellosis. METHODS:
      Recombinant VirB12 protein was prepared and evaluated the efficacy of it in an
      indirect enzyme-linked immunosorbent assay (ELISA) for brucellosis with sera
      collected from different region of Iran and the results were compared with a
      commercial ELISA kit. RESULTS: Sera from human brucellosis patients strongly
      reacted to the purified recombinant VirB12. The sensitivity, specificity,
      accuracy, negative predictive value and positive predictive value of recombinant 
      VirB12-based ELISA related to the commercial-ELISA method were 87.8, 94, 90, 80
      and 96.6% respectively. CONCLUSIONS: We concluded that antigenic VirB12 have a
      property value that can be considered as a candidate for using in serodiagnostic 
      tests for human brucellosis.
FAU - Mirkalantari, Shiva
AU  - Mirkalantari S
AD  - Microbiology Department, Faculty of Medicine, Iran University of Medical
      Sciences, Tehran, Iran.
FAU - Zarnani, Amir-Hassan
AU  - Zarnani AH
AD  - Dept. of Immunology, School of Public Health, Tehran University of Medical
      Sciences, Tehran, Iran.
AD  - Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran.
FAU - Nazari, Mahboobeh
AU  - Nazari M
AD  - Monoclonal Antibody Reaserch Center, Avicenna Research Institute, ACECR, Tehran, 
      Iran.
FAU - Irajian, Gholam Reza
AU  - Irajian GR
AD  - Microbiology Department, Faculty of Medicine, Iran University of Medical
      Sciences, Tehran, Iran.
FAU - Amirmozafari, Nour
AU  - Amirmozafari N
AD  - Microbiology Department, Faculty of Medicine, Iran University of Medical
      Sciences, Tehran, Iran. [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170303
PL  - England
TA  - Ann Clin Microbiol Antimicrob
JT  - Annals of clinical microbiology and antimicrobials
JID - 101152152
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Biomarkers)
RN  - 0 (Immune Sera)
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Type IV Secretion Systems)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/biosynthesis/*blood/isolation & purification
MH  - Antigens, Bacterial/*blood/immunology
MH  - Biomarkers/blood
MH  - Brucella melitensis/immunology/*isolation & purification
MH  - Brucellosis/blood/*diagnosis/immunology/microbiology
MH  - Cloning, Molecular
MH  - Enzyme-Linked Immunosorbent Assay/standards
MH  - Escherichia coli/genetics/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Immune Sera/chemistry
MH  - Membrane Proteins/*blood/immunology
MH  - Predictive Value of Tests
MH  - Rabbits
MH  - Recombinant Proteins/biosynthesis/genetics/immunology
MH  - Serologic Tests/*methods
MH  - Type IV Secretion Systems/immunology
PMC - PMC5335726
OTO - NOTNLM
OT  - Brucella melitensis
OT  - Brucellosis
OT  - Enzyme-linked immunosorbent assay
OT  - Recombinant proteins
OT  - VirB12
EDAT- 2017/03/04 06:00
MHDA- 2017/03/08 06:00
CRDT- 2017/03/04 06:00
PHST- 2016/09/14 [received]
PHST- 2017/02/05 [accepted]
AID - 10.1186/s12941-017-0182-4 [doi]
AID - 10.1186/s12941-017-0182-4 [pii]
PST - epublish
SO  - Ann Clin Microbiol Antimicrob. 2017 Mar 3;16(1):8. doi:
      10.1186/s12941-017-0182-4.

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