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MiR-199a-5p suppresses tumorigenesis by targeting clathrin heavy chain in hepatocellular carcinoma.

Abstract The deregulation of microRNA (miRNA) is frequently associated with a variety of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the expression and possible role of miR-199a-5p in HCC. The expression of miR-199a-5p was measured by quantitative RT-PCR in HCC. The effect of miR-199a-5p was evaluated by cell viability and colony formation assays in HCC cell lines and tumor cell growth assay in xenograft nude mice. Quantitative real time PCR results showed that miR-199a-5p was down-regulated in 77.9 % (67/86) of HCC tissues compared with adjacent nontumor tissues. MiR-199a-5p mimic reduced cell viability and colony formation by induction of cell arrest in HCC cell lines and inhibited tumor cell growth in xenograft nude mice, but miR-199a-5p inhibitor increased cell viability and colony formation in HCC cell lines and tumor cell growth in xenograft nude mice. Furthermore, CLTC was defined as a potential direct target of miR-199a-5p by MiRanda and TargetScan predictions. The dual-luciferase reporter gene assay results showed that CLTC was a direct target of miR-199a-5p. The use of miR-199a-5p mimic or inhibitor could decrease or increase CLTC protein levels in HCC cell lines. We conclude that the frequently down-regulated miR-199a-5p can regulate CLTC and might function as a tumor suppressor in HCC. Therefore, miR-199a-5p may serve as a useful therapeutic agent for miRNA-based HCC therapy.
PMID
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Authors

Mayor MeshTerms

Gene Expression Regulation, Neoplastic

Keywords

CLTC

Hepatocellular carcinoma

cell growth

miR-199a-5p

Journal Title cell biochemistry and function
Publication Year Start




PMID- 28261837
OWN - NLM
STAT- MEDLINE
DA  - 20170306
DCOM- 20170317
LR  - 20170317
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 35
IP  - 2
DP  - 2017 Mar
TI  - MiR-199a-5p suppresses tumorigenesis by targeting clathrin heavy chain in
      hepatocellular carcinoma.
PG  - 98-104
LID - 10.1002/cbf.3252 [doi]
AB  - The deregulation of microRNA (miRNA) is frequently associated with a variety of
      cancers, including hepatocellular carcinoma (HCC). In this study, we investigated
      the expression and possible role of miR-199a-5p in HCC. The expression of
      miR-199a-5p was measured by quantitative RT-PCR in HCC. The effect of miR-199a-5p
      was evaluated by cell viability and colony formation assays in HCC cell lines and
      tumor cell growth assay in xenograft nude mice. Quantitative real time PCR
      results showed that miR-199a-5p was down-regulated in 77.9 % (67/86) of HCC
      tissues compared with adjacent nontumor tissues. MiR-199a-5p mimic reduced cell
      viability and colony formation by induction of cell arrest in HCC cell lines and 
      inhibited tumor cell growth in xenograft nude mice, but miR-199a-5p inhibitor
      increased cell viability and colony formation in HCC cell lines and tumor cell
      growth in xenograft nude mice. Furthermore, CLTC was defined as a potential
      direct target of miR-199a-5p by MiRanda and TargetScan predictions. The
      dual-luciferase reporter gene assay results showed that CLTC was a direct target 
      of miR-199a-5p. The use of miR-199a-5p mimic or inhibitor could decrease or
      increase CLTC protein levels in HCC cell lines. We conclude that the frequently
      down-regulated miR-199a-5p can regulate CLTC and might function as a tumor
      suppressor in HCC. Therefore, miR-199a-5p may serve as a useful therapeutic agent
      for miRNA-based HCC therapy.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Huang, Guo-Hao
AU  - Huang GH
AD  - Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University,
      Guangzhou, China.
FAU - Shan, Hong
AU  - Shan H
AUID- ORCID: http://orcid.org/0000-0002-8896-5813
AD  - Department of Interventional Radiology, The Fifth Affiliated Hospital of Sun
      Yat-sen University, Zhuhai, China.
AD  - Interventional Radiology Institute, Sun Yat-sen University, Zhuhai, China.
FAU - Li, Dan
AU  - Li D
AD  - Department of Interventional Radiology, The Fifth Affiliated Hospital of Sun
      Yat-sen University, Zhuhai, China.
FAU - Zhou, Bin
AU  - Zhou B
AD  - Department of Interventional Radiology, The Fifth Affiliated Hospital of Sun
      Yat-sen University, Zhuhai, China.
AD  - Interventional Radiology Institute, Sun Yat-sen University, Zhuhai, China.
FAU - Pang, Peng-Fei
AU  - Pang PF
AD  - Department of Interventional Radiology, The Fifth Affiliated Hospital of Sun
      Yat-sen University, Zhuhai, China.
AD  - Interventional Radiology Institute, Sun Yat-sen University, Zhuhai, China.
LA  - eng
PT  - Journal Article
DEP - 20170305
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (CLTC protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (mirn199 microRNA, human)
RN  - 114899-12-6 (Clathrin Heavy Chains)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Carcinogenesis/*genetics/metabolism/pathology
MH  - Carcinoma, Hepatocellular/*genetics/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Clathrin Heavy Chains/*genetics/metabolism
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Reporter
MH  - Humans
MH  - Liver Neoplasms/*genetics/metabolism/pathology
MH  - Luciferases/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Neoplasm Transplantation
MH  - Signal Transduction
OTO - NOTNLM
OT  - CLTC
OT  - Hepatocellular carcinoma
OT  - cell growth
OT  - miR-199a-5p
EDAT- 2017/03/07 06:00
MHDA- 2017/03/18 06:00
CRDT- 2017/03/07 06:00
PHST- 2016/11/03 [received]
PHST- 2017/01/01 [revised]
PHST- 2017/01/03 [accepted]
AID - 10.1002/cbf.3252 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2017 Mar;35(2):98-104. doi: 10.1002/cbf.3252. Epub 2017 Mar
      5.

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