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Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves.

Abstract Bacterial endocarditis is a recognised disease in humans and animals. In humans, infection with Coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly in animals. Endocarditis in cattle is a common post-mortem finding in abattoirs and studies have identified Trueperella pyogenes as a major cause. Despite exposure of cattle to C. burnetii, the significance of this particular bacterium for development and progression of endocarditis has not been studied in detail. Cardiac valves of cattle affected with endocarditis (nā€‰=ā€‰100) were examined by histology, fluorescence in situ hybridization (FISH) and real time quantitative polymerase chain reaction (PCR). Serum was examined for anti-C. burnetii antibodies by enzyme-linked immunosorbent assay (ELISA).
PMID
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Authors

Mayor MeshTerms
Keywords

Cattle

Coxiella burnetii

Endocarditis

PCR

Q fever

Journal Title bmc veterinary research
Publication Year Start




PMID- 28274243
OWN - NLM
STAT- MEDLINE
DA  - 20170309
DCOM- 20170316
LR  - 20170316
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 13
IP  - 1
DP  - 2017 Mar 09
TI  - Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves.
PG  - 69
LID - 10.1186/s12917-017-0988-5 [doi]
AB  - BACKGROUND: Bacterial endocarditis is a recognised disease in humans and animals.
      In humans, infection with Coxiella burnetii can cause endocarditis, but this has 
      not been investigated thoroughly in animals. Endocarditis in cattle is a common
      post-mortem finding in abattoirs and studies have identified Trueperella pyogenes
      as a major cause. Despite exposure of cattle to C. burnetii, the significance of 
      this particular bacterium for development and progression of endocarditis has not
      been studied in detail. Cardiac valves of cattle affected with endocarditis (n = 
      100) were examined by histology, fluorescence in situ hybridization (FISH) and
      real time quantitative polymerase chain reaction (PCR). Serum was examined for
      anti-C. burnetii antibodies by enzyme-linked immunosorbent assay (ELISA).
      RESULTS: Serology revealed that 70% of the cattle were positive for antibodies to
      C. burnetii, while PCR analysis identified 25% of endocarditis valve samples as
      being positive. C. burnetii was not detected by FISH, probably due to the low
      infection levels. Most cattle had chronic valvular vegetative endocarditis with
      lesions being characterised by a core of fibrous tissue covered by significant
      amounts of fibrin, sometimes with areas of liquefaction, and with a coagulum
      covering the surface. In a few cases, including the case with the highest
      infection level, lesions were characterized by extensive fibrosis and
      calcification. Histologically, bacteria other than C. burnetii were observed in
      most cases. CONCLUSIONS: The presence of C. burnetii DNA is relatively common in 
      cattle affected with valvular endocarditis. The role of C. burnetii remains
      however unknown as lesions did not differ between C. burnetii infected and
      non-infected cattle and because T. pyogenes-like bacteria were present in the
      inflamed valves; a bacterium able to induce the observed lesions. Heart valves of
      normal cattle should be investigated to assess if C. burnetii may be present
      without preexisting lesions.
FAU - Agerholm, Jorgen S
AU  - Agerholm JS
AD  - Department of Large Animal Sciences, Faculty of Health and Medical Sciences,
      University of Copenhagen, Hojbakkegaard Alle 5, DK-2630, Taastrup, Denmark.
      jager@sund.ku.dk.
FAU - Jensen, Tim K
AU  - Jensen TK
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27,
      DK-1870, Frederiksberg C, Denmark.
FAU - Agger, Jens F
AU  - Agger JF
AD  - Department of Large Animal Sciences, Faculty of Health and Medical Sciences,
      University of Copenhagen, Hojbakkegaard Alle 5, DK-2630, Taastrup, Denmark.
FAU - Engelsma, Marc Y
AU  - Engelsma MY
AD  - Department of Diagnostics and Crisis Organisation, Wageningen Bioveterinary
      Research, Edelhertweg 15, 8219 PH, Lelystad, The Netherlands.
FAU - Roest, Hendrik I J
AU  - Roest HI
AD  - Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research,
      Edelhertweg 15, 8219 PH, Lelystad, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20170309
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Cattle
MH  - Cattle Diseases/*microbiology
MH  - Coxiella burnetii/*genetics
MH  - DNA, Bacterial/*isolation & purification
MH  - Endocarditis, Bacterial/microbiology/*veterinary
MH  - Female
MH  - Heart Valves/*microbiology
MH  - Inflammation/microbiology/veterinary
MH  - Male
MH  - Q Fever/microbiology/*veterinary
PMC - PMC5343293
OTO - NOTNLM
OT  - Cattle
OT  - Coxiella burnetii
OT  - Endocarditis
OT  - PCR
OT  - Q fever
EDAT- 2017/03/10 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/03/10 06:00
PHST- 2016/09/28 [received]
PHST- 2017/03/03 [accepted]
AID - 10.1186/s12917-017-0988-5 [doi]
AID - 10.1186/s12917-017-0988-5 [pii]
PST - epublish
SO  - BMC Vet Res. 2017 Mar 9;13(1):69. doi: 10.1186/s12917-017-0988-5.

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