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CXCL5 Plays a Promoting Role in Osteosarcoma Cell Migration and Invasion in Autocrine- and Paracrine-Dependent Manners.

Abstract CXCL5, a CXC-type chemokine, is an important attractant for granulocytic immune cells by binding to its receptor CXCR2. Recently, CXCL5/CXCR2 has been found to play an oncogenic role in many human cancers. However, the exact role of CXCL5 in osteosarcoma cell migration and invasion has not been revealed. Here we found that the protein expression of CXCL5 was significantly increased in osteosarcoma tissues compared with that in matched adjacent nontumor tissues. Moreover, the expression of CXCL5 was significantly associated with advanced clinical stage and metastasis. Further investigation showed that the CXCL5 expression levels were also significantly increased in osteosarcoma cell lines, including Saos-2, MG63, U2OS, and SW1353, when compared with those in normal osteoblast hFoB1.19 cells. U2OS cells were further transfected with CXCL5-specific siRNA or overexpression plasmid. Knockdown of CXCL5 significantly suppressed U2OS cell migration and invasion. On the contrary, overexpression of CXLC5 remarkably promoted the migration and invasion of U2OS cells. Interestingly, both exogenous CXCL5 treatment and the conditioned medium of CXCL5-overexpressing hFoB1.19 cells could also enhance the migration and invasion of U2OS cells, suggesting that the promoting role of CXCL5 in U2OS cell migration and invasion is also in a paracrine-dependent manner. According to these data, our study demonstrates that CXCL5 is upregulated in osteosarcoma and may play an oncogenic role in osteosarcoma metastasis. Therefore, CXCL5 may become a potential therapeutic target for osteosarcoma treatment.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title oncology research
Publication Year Start




PMID- 28277189
OWN - NLM
STAT- MEDLINE
DA  - 20170309
DCOM- 20170316
LR  - 20170316
IS  - 1555-3906 (Electronic)
IS  - 0965-0407 (Linking)
VI  - 25
IP  - 2
DP  - 2017 Jan 26
TI  - CXCL5 Plays a Promoting Role in Osteosarcoma Cell Migration and Invasion in
      Autocrine- and Paracrine-Dependent Manners.
PG  - 177-186
LID - 10.3727/096504016X14732772150343 [doi]
AB  - CXCL5, a CXC-type chemokine, is an important attractant for granulocytic immune
      cells by binding to its receptor CXCR2. Recently, CXCL5/CXCR2 has been found to
      play an oncogenic role in many human cancers. However, the exact role of CXCL5 in
      osteosarcoma cell migration and invasion has not been revealed. Here we found
      that the protein expression of CXCL5 was significantly increased in osteosarcoma 
      tissues compared with that in matched adjacent nontumor tissues. Moreover, the
      expression of CXCL5 was significantly associated with advanced clinical stage and
      metastasis. Further investigation showed that the CXCL5 expression levels were
      also significantly increased in osteosarcoma cell lines, including Saos-2, MG63, 
      U2OS, and SW1353, when compared with those in normal osteoblast hFoB1.19 cells.
      U2OS cells were further transfected with CXCL5-specific siRNA or overexpression
      plasmid. Knockdown of CXCL5 significantly suppressed U2OS cell migration and
      invasion. On the contrary, overexpression of CXLC5 remarkably promoted the
      migration and invasion of U2OS cells. Interestingly, both exogenous CXCL5
      treatment and the conditioned medium of CXCL5-overexpressing hFoB1.19 cells could
      also enhance the migration and invasion of U2OS cells, suggesting that the
      promoting role of CXCL5 in U2OS cell migration and invasion is also in a
      paracrine-dependent manner. According to these data, our study demonstrates that 
      CXCL5 is upregulated in osteosarcoma and may play an oncogenic role in
      osteosarcoma metastasis. Therefore, CXCL5 may become a potential therapeutic
      target for osteosarcoma treatment.
FAU - Dang, Hongsheng
AU  - Dang H
AD  - Department of Orthopaedics, Taihe Hospital, Hubei University of Medicine, Shiyan,
      Hubei, P.R. China.
FAU - Wu, Wuzhou
AU  - Wu W
FAU - Wang, Bo
AU  - Wang B
FAU - Cui, Cao
AU  - Cui C
FAU - Niu, Juwei
AU  - Niu J
FAU - Chen, Jie
AU  - Chen J
FAU - Chen, Ziqiu
AU  - Chen Z
FAU - Liu, Yi
AU  - Liu Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncol Res
JT  - Oncology research
JID - 9208097
RN  - 0 (CXCL5 protein, human)
RN  - 0 (Chemokine CXCL5)
SB  - IM
MH  - Autocrine Communication/*physiology
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Chemokine CXCL5/*physiology
MH  - Humans
MH  - Neoplasm Invasiveness/*pathology
MH  - Osteosarcoma/metabolism/*pathology
MH  - Paracrine Communication/*physiology
EDAT- 2017/03/10 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/03/10 06:00
AID - 10.3727/096504016X14732772150343 [doi]
PST - ppublish
SO  - Oncol Res. 2017 Jan 26;25(2):177-186. doi: 10.3727/096504016X14732772150343.

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