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Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid Cystic Carcinoma Cells.

Abstract Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to regulate cell biological behavior in some human malignancies. We hypothesized that there was a negative regulation, which might induce apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma (ACC). In this study, the expression levels of miR-222 and the PUMA gene after transfection with antisense miR-222 (As-miR-222) were evaluated by RT-PCR and Western blot assays. Cell proliferation and migratory abilities were detected by CCK-8 and Transwell assays. Cell cycle and apoptosis were analyzed by flow cytometry. Our results showed that, when compared with the control and scramble-transfected groups, the expression of miR-222 in the As-miR-222 group was downregulated, while the expression of PUMA at both mRNA and protein levels was upregulated, cell proliferation and migratory abilities were inhibited, and apoptosis was increased. Our results suggested that As-miR-222 transfection could upregulate the expression of PUMA to induce apoptosis in ACC, providing a new concept for the treatment of ACC.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title oncology research
Publication Year Start




PMID- 28277192
OWN - NLM
STAT- MEDLINE
DA  - 20170309
DCOM- 20170316
LR  - 20170316
IS  - 1555-3906 (Electronic)
IS  - 0965-0407 (Linking)
VI  - 25
IP  - 2
DP  - 2017 Jan 26
TI  - Downregulation of miR-222 Induces Apoptosis and Cellular Migration in Adenoid
      Cystic Carcinoma Cells.
PG  - 207-214
LID - 10.3727/096504016X14732772150460 [doi]
AB  - Previous studies have shown that miR-222 targets the p53 upregulated modulator of
      apoptosis (PUMA) to regulate cell biological behavior in some human malignancies.
      We hypothesized that there was a negative regulation, which might induce
      apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma (ACC). In this
      study, the expression levels of miR-222 and the PUMA gene after transfection with
      antisense miR-222 (As-miR-222) were evaluated by RT-PCR and Western blot assays. 
      Cell proliferation and migratory abilities were detected by CCK-8 and Transwell
      assays. Cell cycle and apoptosis were analyzed by flow cytometry. Our results
      showed that, when compared with the control and scramble-transfected groups, the 
      expression of miR-222 in the As-miR-222 group was downregulated, while the
      expression of PUMA at both mRNA and protein levels was upregulated, cell
      proliferation and migratory abilities were inhibited, and apoptosis was
      increased. Our results suggested that As-miR-222 transfection could upregulate
      the expression of PUMA to induce apoptosis in ACC, providing a new concept for
      the treatment of ACC.
FAU - Zhou, Ziliang
AU  - Zhou Z
AD  - Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of
      Stomatology, Sun Yat-sen University, Guangzhou, P.R. China.
FAU - Zhou, Lijie
AU  - Zhou L
FAU - Jiang, Fangfang
AU  - Jiang F
FAU - Zeng, Binghui
AU  - Zeng B
FAU - Wei, Changbo
AU  - Wei C
FAU - Zhao, Wei
AU  - Zhao W
FAU - Yu, Dongsheng
AU  - Yu D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncol Res
JT  - Oncology research
JID - 9208097
RN  - 0 (MIRN222 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Apoptosis/*physiology
MH  - Carcinoma, Adenoid Cystic/*genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Down-Regulation/*physiology
MH  - Humans
MH  - MicroRNAs/*genetics/metabolism
EDAT- 2017/03/10 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/03/10 06:00
AID - 10.3727/096504016X14732772150460 [doi]
PST - ppublish
SO  - Oncol Res. 2017 Jan 26;25(2):207-214. doi: 10.3727/096504016X14732772150460.

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