PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Knockdown of Collagen Triple Helix Repeat Containing 1 (CTHRC1) Inhibits Epithelial-Mesenchymal Transition and Cellular Migration in Glioblastoma Cells.

Abstract Collagen triple helix repeat containing 1 (CTHRC1), an extracellular matrix-related protein, has been found to be upregulated in many solid tumors and contributes to tumorigenesis. We found that CTHRC1 is overexpressed in glioblastoma tissues and cells. By using the technique of RNA interference, the expression of CTHRC1 in the human glioblastoma U-87MG cell line was downregulated, and the proliferation and migration of U-87MG cells were examined. The results showed that the knockdown of CTHRC1 exerts inhibitory effects on the proliferation and migration ability of U-87MG cells. Knockdown of CTHRC1 expression in U-87MG cells resulted in upregulation in the expression of E-cadherin and downregulation in the expression of N-cadherin, SNAIL, and Slug, suggesting that CTHRC1 inhibits glioblastoma cell migration by suppressing epithelial-mesenchymal transition (EMT). Knockdown of CTHRC1 led to remarkably decreased β-catenin protein levels in the nucleus. These results indicate that CTHRC1 might play an important role in the development of glioblastoma and offer a candidate molecular target for glioblastoma prevention and therapy.
PMID
Related Publications

Lentivirus-Mediated Knockdown of CTHRC1 Inhibits Osteosarcoma Cell Proliferation and Migration.

Role of CTHRC1 in proliferation, migration and invasion of human colorectal cancer cells.

MicroRNA-663 inhibits the proliferation, migration and invasion of glioblastoma cells via targeting TGF-β1.

High expression of CTHRC1 promotes EMT of epithelial ovarian cancer (EOC) and is associated with poor prognosis.

Knockdown of Collagen Triple Helix Repeat Containing-1 Inhibits the Proliferation and Epithelial-to-Mesenchymal Transition in Renal Cell Carcinoma Cells.

Authors

Mayor MeshTerms

Gene Knockdown Techniques

Keywords
Journal Title oncology research
Publication Year Start




PMID- 28277194
OWN - NLM
STAT- MEDLINE
DA  - 20170309
DCOM- 20170316
LR  - 20170316
IS  - 1555-3906 (Electronic)
IS  - 0965-0407 (Linking)
VI  - 25
IP  - 2
DP  - 2017 Jan 26
TI  - Knockdown of Collagen Triple Helix Repeat Containing 1 (CTHRC1) Inhibits
      Epithelial-Mesenchymal Transition and Cellular Migration in Glioblastoma Cells.
PG  - 225-232
LID - 10.3727/096504016X14732772150587 [doi]
AB  - Collagen triple helix repeat containing 1 (CTHRC1), an extracellular
      matrix-related protein, has been found to be upregulated in many solid tumors and
      contributes to tumorigenesis. We found that CTHRC1 is overexpressed in
      glioblastoma tissues and cells. By using the technique of RNA interference, the
      expression of CTHRC1 in the human glioblastoma U-87MG cell line was
      downregulated, and the proliferation and migration of U-87MG cells were examined.
      The results showed that the knockdown of CTHRC1 exerts inhibitory effects on the 
      proliferation and migration ability of U-87MG cells. Knockdown of CTHRC1
      expression in U-87MG cells resulted in upregulation in the expression of
      E-cadherin and downregulation in the expression of N-cadherin, SNAIL, and Slug,
      suggesting that CTHRC1 inhibits glioblastoma cell migration by suppressing
      epithelial-mesenchymal transition (EMT). Knockdown of CTHRC1 led to remarkably
      decreased beta-catenin protein levels in the nucleus. These results indicate that
      CTHRC1 might play an important role in the development of glioblastoma and offer 
      a candidate molecular target for glioblastoma prevention and therapy.
FAU - Liu, Jianpeng
AU  - Liu J
AD  - Department of Neurotrauma, The First Hospital of Jilin University, Changchun,
      Jilin Province, P.R. China.
FAU - Li, Wei
AU  - Li W
FAU - Liu, Shunshun
AU  - Liu S
FAU - Zheng, Xu
AU  - Zheng X
FAU - Shi, Lin
AU  - Shi L
FAU - Zhang, Weitao
AU  - Zhang W
FAU - Yang, Hongfa
AU  - Yang H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncol Res
JT  - Oncology research
JID - 9208097
RN  - 0 (CTHRC1 protein, human)
RN  - 0 (Extracellular Matrix Proteins)
SB  - IM
MH  - Adult
MH  - Brain Neoplasms/*genetics/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - Epithelial-Mesenchymal Transition/*genetics
MH  - Extracellular Matrix Proteins/deficiency/*genetics
MH  - Female
MH  - *Gene Knockdown Techniques/methods
MH  - Glioblastoma/*genetics/metabolism/pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
EDAT- 2017/03/10 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/03/10 06:00
AID - 10.3727/096504016X14732772150587 [doi]
PST - ppublish
SO  - Oncol Res. 2017 Jan 26;25(2):225-232. doi: 10.3727/096504016X14732772150587.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>