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Induction of Apoptosis by Berberine in Hepatocellular Carcinoma HepG2 Cells via Downregulation of NF-κB.

Abstract Hepatocellular carcinoma (HCC) is highly resistant to traditional chemotherapeutic approaches, which causes difficulty in the development of effective drugs for the treatment of HCC. Berberine, a major ingredient of Rhizoma coptidis, is a natural alkaloid used in traditional Chinese medicine. Berberine exhibits potent antitumor activity against HCC due to its high efficiency and low toxicity. In the present study, we found that berberine sensitized HepG cells to NF-κB-mediated apoptosis. Berberine exhibited a significant antiproliferation effect on the HepG2 cells and promoted apoptosis. Both qRT-PCR and immunofluorescence staining revealed that berberine reduced the NF-κB p65 levels in HepG2 cells. Moreover, p65 overexpression rescued berberine-induced cell proliferation and prevented HepG2 cells from undergoing apoptosis. These results suggest that berberine inhibits the growth of HepG2 cells by promoting apoptosis through the NF-κB p65 pathway.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title oncology research
Publication Year Start




PMID- 28277195
OWN - NLM
STAT- MEDLINE
DA  - 20170309
DCOM- 20170316
LR  - 20170316
IS  - 1555-3906 (Electronic)
IS  - 0965-0407 (Linking)
VI  - 25
IP  - 2
DP  - 2017 Jan 26
TI  - Induction of Apoptosis by Berberine in Hepatocellular Carcinoma HepG2 Cells via
      Downregulation of NF-kappaB.
PG  - 233-239
LID - 10.3727/096504016X14742891049073 [doi]
AB  - Hepatocellular carcinoma (HCC) is highly resistant to traditional
      chemotherapeutic approaches, which causes difficulty in the development of
      effective drugs for the treatment of HCC. Berberine, a major ingredient of
      Rhizoma coptidis, is a natural alkaloid used in traditional Chinese medicine.
      Berberine exhibits potent antitumor activity against HCC due to its high
      efficiency and low toxicity. In the present study, we found that berberine
      sensitized HepG cells to NF-kappaB-mediated apoptosis. Berberine exhibited a
      significant antiproliferation effect on the HepG2 cells and promoted apoptosis.
      Both qRT-PCR and immunofluorescence staining revealed that berberine reduced the 
      NF-kappaB p65 levels in HepG2 cells. Moreover, p65 overexpression rescued
      berberine-induced cell proliferation and prevented HepG2 cells from undergoing
      apoptosis. These results suggest that berberine inhibits the growth of HepG2
      cells by promoting apoptosis through the NF-kappaB p65 pathway.
FAU - Li, Min
AU  - Li M
AD  - Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical
      University, Guangzhou, Guangdong, P.R. China.
FAU - Zhang, Mao
AU  - Zhang M
FAU - Zhang, Zhi-Lang
AU  - Zhang ZL
FAU - Liu, Ning
AU  - Liu N
FAU - Han, Xiao-Yu
AU  - Han XY
FAU - Liu, Qin-Cheng
AU  - Liu QC
FAU - Deng, Wei-Jun
AU  - Deng WJ
FAU - Liao, Cai-Xian
AU  - Liao CX
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncol Res
JT  - Oncology research
JID - 9208097
RN  - 0 (NF-kappa B)
RN  - 0I8Y3P32UF (Berberine)
SB  - IM
MH  - Apoptosis/drug effects/*physiology
MH  - Berberine/*pharmacology/therapeutic use
MH  - Carcinoma, Hepatocellular/drug therapy/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/drug effects/*physiology
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver Neoplasms/drug therapy/*metabolism
MH  - NF-kappa B/antagonists & inhibitors/*metabolism
EDAT- 2017/03/10 06:00
MHDA- 2017/03/17 06:00
CRDT- 2017/03/10 06:00
AID - 10.3727/096504016X14742891049073 [doi]
PST - ppublish
SO  - Oncol Res. 2017 Jan 26;25(2):233-239. doi: 10.3727/096504016X14742891049073.

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