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B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera.

Abstract Identifying antigenic proteins and mapping their epitopes is important for the development of diagnostic reagents and recombinant vaccines. B-cell epitopes of African horse sickness virus (AHSV) have previously been mapped on VP2, VP5, VP7 and NS1, using mouse, rabbit and chicken monoclonal antibodies. A comprehensive study of the humoral immune response of five vaccinated horses to AHSV-4 antigenic peptides was undertaken. A fragmented-genome phage display library expressing a repertoire of AHSV-4 peptides spanning the entire genome was constructed. The library was affinity selected for binders on immobilised polyclonal immunoglobulin G (IgG) isolated from horse sera collected pre- and post-immunisation with an attenuated AHSV-4 monovalent vaccine. The DNA inserts of binding phages were sequenced with Illumina high-throughput sequencing. The data were normalised using preimmune IgG-selected sequences. More sequences mapped to the genes coding for NS3, VP6 and VP5 than to the other genes. However, VP2 and VP5 each had more antigenic regions than each of the other proteins. This study identified a number of epitopes to which the horse's humoral immune system responds during immunisation with AHSV-4.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title the onderstepoort journal of veterinary research
Publication Year Start




PMID- 28281773
OWN - NLM
STAT- MEDLINE
DA  - 20170310
DCOM- 20170317
LR  - 20170317
IS  - 2219-0635 (Electronic)
IS  - 0030-2465 (Linking)
VI  - 84
IP  - 1
DP  - 2017 Feb 24
TI  - B-cell epitopes of African horse sickness virus serotype 4 recognised by immune
      horse sera.
PG  - e1-e12
LID - 10.4102/ojvr.v84i1.1313 [doi]
AB  - Identifying antigenic proteins and mapping their epitopes is important for the
      development of diagnostic reagents and recombinant vaccines. B-cell epitopes of
      African horse sickness virus (AHSV) have previously been mapped on VP2, VP5, VP7 
      and NS1, using mouse, rabbit and chicken monoclonal antibodies. A comprehensive
      study of the humoral immune response of five vaccinated horses to AHSV-4
      antigenic peptides was undertaken. A fragmented-genome phage display library
      expressing a repertoire of AHSV-4 peptides spanning the entire genome was
      constructed. The library was affinity selected for binders on immobilised
      polyclonal immunoglobulin G (IgG) isolated from horse sera collected pre- and
      post-immunisation with an attenuated AHSV-4 monovalent vaccine. The DNA inserts
      of binding phages were sequenced with Illumina high-throughput sequencing. The
      data were normalised using preimmune IgG-selected sequences. More sequences
      mapped to the genes coding for NS3, VP6 and VP5 than to the other genes. However,
      VP2 and VP5 each had more antigenic regions than each of the other proteins. This
      study identified a number of epitopes to which the horse's humoral immune system 
      responds during immunisation with AHSV-4.
FAU - Mathebula, Evans M
AU  - Mathebula EM
FAU - Faber, Frederika E
AU  - Faber FE
FAU - Van Wyngaardt, Wouter
AU  - Van Wyngaardt W
FAU - Van Schalkwyk, Antoinette
AU  - Van Schalkwyk A
FAU - Pretorius, Alri
AU  - Pretorius A
FAU - Fehrsen, Jeanni
AU  - Fehrsen J
AD  - New Generation Vaccines Programme, Agricultural Research Council - Onderstepoort 
      Veterinary Institute; Department of Veterinary Tropical Diseases, University of
      Pretoria. fehrsenj@arc.agric.za.
LA  - eng
PT  - Journal Article
DEP - 20170224
PL  - South Africa
TA  - Onderstepoort J Vet Res
JT  - The Onderstepoort journal of veterinary research
JID - 0401107
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Immune Sera)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - African Horse Sickness/blood/immunology/*prevention & control/virology
MH  - African Horse Sickness Virus/*immunology
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes, B-Lymphocyte/*immunology
MH  - Horses
MH  - Immune Sera/*immunology
MH  - Immunoglobulin G/immunology
MH  - Vaccination/veterinary
MH  - Viral Vaccines/*administration & dosage
EDAT- 2017/03/11 06:00
MHDA- 2017/03/18 06:00
CRDT- 2017/03/11 06:00
PHST- 2016/07/13 [received]
PHST- 2016/09/29 [accepted]
PHST- 2016/09/27 [revised]
AID - 1313 [pii]
PST - epublish
SO  - Onderstepoort J Vet Res. 2017 Feb 24;84(1):e1-e12. doi: 10.4102/ojvr.v84i1.1313.

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