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Molecular characterization of cryptosporidium in children aged 0- 5 years with diarrhea in Jos, Nigeria.

Abstract Cryptosporidium is an important cause of diarrhea in children and immune-compromised individuals. Recent advances in molecular diagnostics have led to the discovery of subtype families that are thought to be more commonly associated with diarrhea. We aimed to isolate and characterize Cryptosporidium spp among children with diarrhea in Jos, Nigeria.
PMID
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Authors

Mayor MeshTerms
Keywords

Children

Nigeria

PCR-RFLP

cryptosporidium

diarrhea

genotypes

molecular characterization

Journal Title the pan african medical journal
Publication Year Start




PMID- 28293369
OWN - NLM
STAT- MEDLINE
DA  - 20170315
DCOM- 20170329
LR  - 20170330
IS  - 1937-8688 (Electronic)
VI  - 25
DP  - 2016
TI  - Molecular characterization of cryptosporidium in children aged 0- 5 years with
      diarrhea in Jos, Nigeria.
PG  - 253
LID - 10.11604/pamj.2016.25.253.10018 [doi]
AB  - INTRODUCTION: Cryptosporidium is an important cause of diarrhea in children and
      immune-compromised individuals. Recent advances in molecular diagnostics have led
      to the discovery of subtype families that are thought to be more commonly
      associated with diarrhea. We aimed to isolate and characterize Cryptosporidium
      spp among children with diarrhea in Jos, Nigeria. METHODS: Stool samples were
      collected from165 children aged 0-5 years with diarrhea. Cryptosporidium oocysts 
      were examined by wet mount preparation, using formalin ether and a modified acid 
      fast staining method. DNA was extracted from positive samples using QIAamp DNA
      stool mini kit and PCR-RFLP assay was carried out after quantification.
      Genotyping and phylogenetic analysis were done to determine the subtype families 
      and their relatedness. RESULTS: From the 165 children studied, 8 (4.8%) were
      infected with Cryptosporidium. PCR-RFLP assay and genotype characterization found
      the following Cryptosporidium species: C. hominis 6 (75%) and C. parvum 2
      (25.0%), with family subtypes Id-5, Ie-1 and IIa-1, IId-1 respectively.The most
      common species was C. hominis and the frequent subtype was C. hominis-Id 5
      (62.5%). CONCLUSION: Cryptosporidium is not an uncommon cause of diarrhea in
      children, with C. hominis being the dominant species. Also C. hominis Id is the
      commonest sub-family subtype. Put together, zoonotic species may be an important 
      cause of diarrhea in children aged 0-5 years in Jos, Nigeria.
FAU - Anejo-Okopi, Joseph Aje
AU  - Anejo-Okopi JA
AD  - Department of Microbiology, University of Jos, Jos, Nigeria; AIDS Prevention
      Initiative in Nigeria, Jos University Teaching Hospital, Jos, Nigeria.
FAU - Okojokwu, Julius Ocheme
AU  - Okojokwu JO
AD  - Department of Microbiology, University of Jos, Jos, Nigeria.
FAU - Ebonyi, Augustine Odo
AU  - Ebonyi AO
AD  - AIDS Prevention Initiative in Nigeria, Jos University Teaching Hospital, Jos,
      Nigeria; Department of Pediatrics, University of Jos, Jos, Nigeria.
FAU - Ejeliogu, Emeka Uba
AU  - Ejeliogu EU
AD  - AIDS Prevention Initiative in Nigeria, Jos University Teaching Hospital, Jos,
      Nigeria; Department of Pediatrics, University of Jos, Jos, Nigeria.
FAU - Isa, Samson Ejiji
AU  - Isa SE
AD  - AIDS Prevention Initiative in Nigeria, Jos University Teaching Hospital, Jos,
      Nigeria; Department of Medicine, University of Jos, Jos, Nigeria.
FAU - Audu, Onyemocho
AU  - Audu O
AD  - Department of Epidemiology and Community Health, College of Health Sciences,
      Benue State University, Makurdi, Nigeria.
FAU - Akpakpan, Edoama Edet
AU  - Akpakpan EE
AD  - Department of Biological Science, Federal University, Otuoke, Bayelsa State,
      Nigeria.
FAU - Nwachukwu, Esther Ebere
AU  - Nwachukwu EE
AD  - Department of Microbiology, University of Jos, Jos, Nigeria.
FAU - Ifokwe, Christabel Kelechi
AU  - Ifokwe CK
AD  - Department of Microbiology, University of Jos, Jos, Nigeria.
FAU - Ali, Murna
AU  - Ali M
AD  - Department of Microbiology, University of Jos, Jos, Nigeria.
FAU - Lar, Patricia
AU  - Lar P
AD  - Department of Microbiology, University of Jos, Jos, Nigeria.
FAU - Oguche, Stephen
AU  - Oguche S
AD  - AIDS Prevention Initiative in Nigeria, Jos University Teaching Hospital, Jos,
      Nigeria; Department of Pediatrics, University of Jos, Jos, Nigeria.
LA  - eng
PT  - Journal Article
DEP - 20161221
PL  - Uganda
TA  - Pan Afr Med J
JT  - The Pan African medical journal
JID - 101517926
SB  - IM
MH  - Child, Preschool
MH  - Cross-Sectional Studies
MH  - Cryptosporidiosis/*epidemiology
MH  - Cryptosporidium/*isolation & purification
MH  - Diarrhea/epidemiology/*parasitology
MH  - Feces/parasitology
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Nigeria/epidemiology
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
PMC - PMC5337289
OTO - NOTNLM
OT  - Children
OT  - Nigeria
OT  - PCR-RFLP
OT  - cryptosporidium
OT  - diarrhea
OT  - genotypes
OT  - molecular characterization
COI - The authors declare no competing interests.
EDAT- 2017/03/16 06:00
MHDA- 2017/03/31 06:00
CRDT- 2017/03/16 06:00
PHST- 2016/06/10 [received]
PHST- 2016/09/22 [accepted]
AID - 10.11604/pamj.2016.25.253.10018 [doi]
AID - PAMJ-25-253 [pii]
PST - epublish
SO  - Pan Afr Med J. 2016 Dec 21;25:253. doi: 10.11604/pamj.2016.25.253.10018.
      eCollection 2016.

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