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Immunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumors.

Abstract Matrix metalloproteases (MMPs) are endogenous proteases that are responsible for degradation of extracellular matrix (ECM) proteins and cell surface antigens. The breakdown of ECM participates in the local invasion and distant metastases of malignant tumors. Canine transmissible venereal tumor (CTVT) is a naturally occurring contagious round cell neoplasm of dogs that affects mainly the external genitalia of both sexes. CTVT generally is a locally invasive tumor, but distant metastases also are common in puppies and immunocompromised dogs. We investigated the immune expressions and activities of MMP-2 and MMP-9 in CTVT. The presence of these enzymes in tumor cells and tissue homogenates was demonstrated by immunohistochemistry and western blotting. We used gelatin substrate zymography to evaluate the activities of MMP-2 and MMP-9 enzymes in tumor homogenates. We found that tumor cells expressed both MMP-2 and MMP-9. Electrophoretic bands corresponding to MMP-9 and MMP-2 were identified in immunoblots and clear bands that corresponded to the active forms of MMP-2 and MMP-9 also were detected in gelatin zymograms. Our study is the first detailed documentation of MMPs in CTVT.
PMID
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Authors

Mayor MeshTerms
Keywords

canine

dog

immunohistochemistry

matrix metalloproteinase

venereal tumor

Journal Title biotechnic & histochemistry : official publication of the biological stain commission
Publication Year Start




PMID- 28296543
OWN - NLM
STAT- MEDLINE
DA  - 20170315
DCOM- 20170330
LR  - 20170330
IS  - 1473-7760 (Electronic)
IS  - 1052-0295 (Linking)
VI  - 92
IP  - 2
DP  - 2017
TI  - Immunocharacterization of matrix metalloproteinase-2 and matrix
      metalloproteinase-9 in canine transmissible venereal tumors.
PG  - 100-106
LID - 10.1080/10520295.2016.1259500 [doi]
AB  - Matrix metalloproteases (MMPs) are endogenous proteases that are responsible for 
      degradation of extracellular matrix (ECM) proteins and cell surface antigens. The
      breakdown of ECM participates in the local invasion and distant metastases of
      malignant tumors. Canine transmissible venereal tumor (CTVT) is a naturally
      occurring contagious round cell neoplasm of dogs that affects mainly the external
      genitalia of both sexes. CTVT generally is a locally invasive tumor, but distant 
      metastases also are common in puppies and immunocompromised dogs. We investigated
      the immune expressions and activities of MMP-2 and MMP-9 in CTVT. The presence of
      these enzymes in tumor cells and tissue homogenates was demonstrated by
      immunohistochemistry and western blotting. We used gelatin substrate zymography
      to evaluate the activities of MMP-2 and MMP-9 enzymes in tumor homogenates. We
      found that tumor cells expressed both MMP-2 and MMP-9. Electrophoretic bands
      corresponding to MMP-9 and MMP-2 were identified in immunoblots and clear bands
      that corresponded to the active forms of MMP-2 and MMP-9 also were detected in
      gelatin zymograms. Our study is the first detailed documentation of MMPs in CTVT.
FAU - Akkoc, A
AU  - Akkoc A
AD  - a Departments of Pathology.
FAU - Nak, D
AU  - Nak D
AD  - b Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Uludag
      , Gorukle , Bursa , Turkey.
FAU - Demirer, A
AU  - Demirer A
AD  - a Departments of Pathology.
FAU - Simsek, G
AU  - Simsek G
AD  - b Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Uludag
      , Gorukle , Bursa , Turkey.
LA  - eng
PT  - Journal Article
DEP - 20170226
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain
      Commission
JID - 9107378
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Dog Diseases/*immunology/pathology
MH  - Dogs
MH  - Female
MH  - Immunohistochemistry/methods
MH  - Male
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
MH  - Venereal Tumors, Veterinary/*metabolism
OTO - NOTNLM
OT  - canine
OT  - dog
OT  - immunohistochemistry
OT  - matrix metalloproteinase
OT  - venereal tumor
EDAT- 2017/03/16 06:00
MHDA- 2017/03/31 06:00
CRDT- 2017/03/16 06:00
AID - 10.1080/10520295.2016.1259500 [doi]
PST - ppublish
SO  - Biotech Histochem. 2017;92(2):100-106. doi: 10.1080/10520295.2016.1259500. Epub
      2017 Feb 26.

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