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Evaluation of loop-mediated isothermal amplification assay for rapid diagnosis of Acanthamoeba keratitis.

Abstract The clinical features of Acanthamoeba keratitis (AK) are non-specific and closely resemble bacterial, viral and fungal keratitis.
PMID
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Rapid and sensitive diagnosis of Acanthamoeba keratitis by loop-mediated isothermal amplification.

Authors

Mayor MeshTerms
Keywords
Journal Title indian journal of medical microbiology
Publication Year Start




PMID- 28303825
OWN - NLM
STAT- MEDLINE
DA  - 20170317
DCOM- 20170413
LR  - 20170413
IS  - 1998-3646 (Electronic)
IS  - 0255-0857 (Linking)
VI  - 35
IP  - 1
DP  - 2017 Jan-Mar
TI  - Evaluation of loop-mediated isothermal amplification assay for rapid diagnosis of
      Acanthamoeba keratitis.
PG  - 90-94
LID - 10.4103/ijmm.IJMM_16_227 [doi]
AB  - BACKGROUND: The clinical features of Acanthamoeba keratitis (AK) are non-specific
      and closely resemble bacterial, viral and fungal keratitis. MATERIALS AND
      METHODS: We compared loop-mediated isothermal amplification (LAMP) with
      microscopy, non-nutrient agar (NNA) culture and polymerase chain reaction (PCR)
      in clinical suspects of AK. RESULTS: Of 52 clinical samples (42 AK suspects and
      10 proven bacterial, viral or fungal keratitis), 3 were positive by direct
      microscopy (sensitivity 60%, confidence interval [CI]: 17%-92.7%), and 5 by NNA
      culture, 18S rDNA PCR and LAMP (sensitivity 100%, CI: 46.3%-100%). The limit of
      detection of Acanthamoeba DNA was 1 pg/mul by both LAMP and PCR. CONCLUSION: PCR 
      and LAMP assays targeting 18S rDNA gene were found particularly suitable for a
      rapid and accurate diagnosis of AK. LAMP assay takes 2-3 h lesser than PCR, and
      thus offers a rapid, highly sensitive and specific, simple and affordable
      diagnostic modality for patients suspected of AK, especially in resource limited 
      settings.
FAU - Mewara, Abhishek
AU  - Mewara A
AD  - Department of Medical Parasitology, Postgraduate Institute of Medical Education
      and Research, Chandigarh, India.
FAU - Khurana, Sumeeta
AU  - Khurana S
AD  - Department of Medical Parasitology, Postgraduate Institute of Medical Education
      and Research, Chandigarh, India.
FAU - Yoonus, Shakila
AU  - Yoonus S
AD  - Department of Medical Parasitology, Postgraduate Institute of Medical Education
      and Research, Chandigarh, India.
FAU - Megha, Kirti
AU  - Megha K
AD  - Department of Medical Parasitology, Postgraduate Institute of Medical Education
      and Research, Chandigarh, India.
FAU - Tanwar, Parveen
AU  - Tanwar P
AD  - Department of Medical Parasitology, Postgraduate Institute of Medical Education
      and Research, Chandigarh, India.
FAU - Gupta, Amit
AU  - Gupta A
AD  - Department of Ophthalmology, Postgraduate Institute of Medical Education and
      Research, Chandigarh, India.
FAU - Sehgal, Rakesh
AU  - Sehgal R
AD  - Department of Medical Parasitology, Postgraduate Institute of Medical Education
      and Research, Chandigarh, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Med Microbiol
JT  - Indian journal of medical microbiology
JID - 8700903
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Acanthamoeba/genetics/*isolation & purification
MH  - Acanthamoeba Keratitis/*diagnosis
MH  - Humans
MH  - Microbiological Techniques/methods
MH  - Microscopy/methods
MH  - Molecular Diagnostic Techniques/*methods
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - RNA, Ribosomal, 18S/genetics
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2017/03/18 06:00
MHDA- 2017/04/14 06:00
CRDT- 2017/03/18 06:00
AID - IndianJMedMicrobiol_2017_35_1_90_202336 [pii]
AID - 10.4103/ijmm.IJMM_16_227 [doi]
PST - ppublish
SO  - Indian J Med Microbiol. 2017 Jan-Mar;35(1):90-94. doi: 10.4103/ijmm.IJMM_16_227.

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