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Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected food-borne pathogen in Brazil.

Abstract Purpose and methodology.Campylobacter jejuni is a major zoonotic pathogen that causes food-borne gastroenteritis worldwide. However, there are only a few studies available that have molecularly characterized C. jejuni strains isolated in Brazil. The aim of this study was to genotype 111 C. jejuni strains isolated from sick humans (43), monkey faeces (19), chicken faeces (14), chicken meat (33) and sewage (2) between 1996 and 2016 in Brazil using flaA-SVR (short variable region) sequencing and PFGE. Furthermore, the presence of 16 virulence genes was analysed by PCR.
PMID
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Authors

Mayor MeshTerms

Genetic Variation

Keywords
Journal Title journal of medical microbiology
Publication Year Start




PMID- 28317494
OWN - NLM
STAT- MEDLINE
DA  - 20170320
DCOM- 20170411
LR  - 20170411
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 66
IP  - 3
DP  - 2017 Mar
TI  - Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected
      food-borne pathogen in Brazil.
PG  - 350-359
LID - 10.1099/jmm.0.000424 [doi]
AB  - Purpose and methodology.Campylobacter jejuni is a major zoonotic pathogen that
      causes food-borne gastroenteritis worldwide. However, there are only a few
      studies available that have molecularly characterized C. jejuni strains isolated 
      in Brazil. The aim of this study was to genotype 111 C. jejuni strains isolated
      from sick humans (43), monkey faeces (19), chicken faeces (14), chicken meat (33)
      and sewage (2) between 1996 and 2016 in Brazil using flaA-SVR (short variable
      region) sequencing and PFGE. Furthermore, the presence of 16 virulence genes was 
      analysed by PCR. RESULTS: Using PFGE and flaA-SVR sequencing, the 111 C. jejuni
      strains studied were grouped into three and two clusters, respectively, and some 
      strains of different origin presented a similarity of >/=80 %. In total, 35
      flaA-SVR alleles were detected. Alleles gt45, gt49 and gt57 were the most
      prevalent, in contrast with those frequently described in the PubMLST database.
      All 111 C. jejuni strains contained the genes flaA, flhA, cadF, docA, cdtA, cdtB,
      cdtC, iamA, ciaB, sodB, dnaJ, pldA, racR and csrA. The wlaN gene was detected in 
      11 strains (9.9 %), and the virB11 in just one strain (0.9 %). CONCLUSIONS: In
      conclusion, the pathogenic potential of the C. jejuni strains studied was
      highlighted by the high frequency of the majority of the virulence genes
      searched. The flaA-SVR sequencing and PFGE results showed that some of the
      strains studied presented a high genotypic similarity, suggesting potential for
      transmission between animal sources and humans in this country. Altogether, the
      results characterize further C. jejuni isolates from Brazil, an important
      producer and exporter of chicken meat.
FAU - Frazao, Miliane Rodrigues
AU  - Frazao MR
AD  - 1Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao
      Preto, SP, Brazil.
FAU - Medeiros, Marta Ines Cazentini
AU  - Medeiros MI
AD  - 2Instituto Adolfo Lutz de Ribeirao Preto, Ribeirao Preto, SP, Brazil.
FAU - Duque, Sheila da Silva
AU  - Duque SD
AD  - 3Fundacao Oswaldo Cruz (Fiocruz), Instituto Oswaldo Cruz, Rio de Janeiro, RJ,
      Brazil.
FAU - Falcao, Juliana Pfrimer
AU  - Falcao JP
AD  - 1Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao
      Preto, SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170320
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Sewage)
RN  - 0 (Virulence Factors)
RN  - 12777-81-0 (Flagellin)
RN  - 133606-66-3 (flaA protein, bacteria)
SB  - IM
MH  - Animals
MH  - Brazil/epidemiology
MH  - Campylobacter Infections/epidemiology/*microbiology/transmission
MH  - Campylobacter jejuni/*genetics/*pathogenicity
MH  - Chickens/microbiology
MH  - Feces/microbiology
MH  - Flagellin/genetics
MH  - Foodborne Diseases/epidemiology/*microbiology/prevention & control
MH  - Gastroenteritis/epidemiology/microbiology
MH  - Genes, Bacterial
MH  - *Genetic Variation
MH  - Genotype
MH  - Haplorhini/microbiology
MH  - Humans
MH  - Neglected Diseases/epidemiology/microbiology
MH  - Polymerase Chain Reaction
MH  - Poultry/microbiology
MH  - Prevalence
MH  - Sequence Analysis, DNA
MH  - Sewage/microbiology
MH  - Virulence
MH  - Virulence Factors/*genetics
EDAT- 2017/03/21 06:00
MHDA- 2017/04/12 06:00
CRDT- 2017/03/21 06:00
AID - 10.1099/jmm.0.000424 [doi]
PST - ppublish
SO  - J Med Microbiol. 2017 Mar;66(3):350-359. doi: 10.1099/jmm.0.000424. Epub 2017 Mar
      20.

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