PubTransformer

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PMID- 28318165
OWN - NLM
STAT- MEDLINE
DA  - 20170320
DCOM- 20170405
LR  - 20170405
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 34
IP  - 6
DP  - 2016 Dec 01
TI  - [Effects of RhoA silencing on proliferation of tongue squamous cancer cells].
PG  - 620-625
LID - 10.7518/hxkq.2016.06.014 [doi]
AB  - OBJECTIVE: This study investigated the effect of RhoA silencing through RNA
      interference on proliferation and growth of tongue cancer cells, as well as
      explored the possible mechanisms of this effect. METHODS: SSC-4 tongue cancer
      cells were cultured in vitro and then transfected with small interfering RNA to
      knock down RhoA expression. The tested cells were divided into three groups:
      experimental group (experimental group 1: transfected with RhoA-siRNA-1;
      experi-mental group 2: transfected with RhoA-siRNA-2), negative control group
      (transfected by random sequence NC-siRNA), and blank control group (transfected
      with Lipofectamine). The expression levels of RhoA mRNA were respectively
      measured by quantitative real-time polymerase chain reaction and western blot
      assay. Moreover, the expression levels of cyclin D1, p21, and p27 and RhoA
      protein were evaluated by Western blot assay. Proliferation and growth
      potentiality were analyzed through evaluation of doubling times and methyl
      thiazolyl tetrazolium assessment. RESULTS: The expression levels of RhoA gene and
      protein of experimental groups significantly decreased following siRNA
      transfection compared with those in the negative and blank control groups. The
      expression of cyclin D1 decreased significantly and that of p21 and p27 increased
      significantly. The doubling time was extended and the growth potentiality
      decreased. CONCLUSIONS: The results indicated that RhoA silencing can inhibit
      proliferation of tongue cancer cells, whereas RhoA affects cell proliferation by 
      regulating the cell cycle pathway. Thus, RhoA is a potential target in gene
      therapy for tongue cancer.
FAU - Guoxin, Yan
AU  - Guoxin Y
AD  - Dept. of Stomatology, Wuxi No 2. People's Hospital, Wuxi 214002, China.
FAU - Bing, Fan
AU  - Bing F
AD  - Dept. of Stomatology, Wuxi No 2. People's Hospital, Wuxi 214002, China.
FAU - Ronghai, Zou
AU  - Ronghai Z
AD  - Dept. of Stomatology, Wuxi No 2. People's Hospital, Wuxi 214002, China.
FAU - Jian, Zhang
AU  - Jian Z
AD  - Dept. of Stomatology, Wuxi No 2. People's Hospital, Wuxi 214002, China.
FAU - Xiaofeng, Sun
AU  - Xiaofeng S
AD  - Dept. of Stomatology, Wuxi No 2. People's Hospital, Wuxi 214002, China.
FAU - Lei, Tong
AU  - Lei T
AD  - Center of Stomatology, Qingdao Municipal Hospital Affiliated to Qingdao
      University Medical College, Qingdao 266071, China.
FAU - Qimin, Wang
AU  - Qimin W
AD  - Center of Stomatology, Qingdao Municipal Hospital Affiliated to Qingdao
      University Medical College, Qingdao 266071, China.
FAU - Jinhong, Han
AU  - Jinhong H
AD  - Center of Stomatology, Qingdao Municipal Hospital Affiliated to Qingdao
      University Medical College, Qingdao 266071, China.
FAU - Xufei, Lu
AU  - Xufei L
AD  - Dept. of Stomatology, Pudong Healthcare Center of Jimo County, Qingdao 266234,
      China.
FAU - Ying, Wang
AU  - Ying W
AD  - College of Stomatology, Weifang Medical University, Weifang 261021, China.
FAU - Yuan, Zhou
AU  - Yuan Z
AD  - College of Stomatology, Weifang Medical University, Weifang 261021, China.
FAU - Zongxuan, He
AU  - Zongxuan H
AD  - Center of Stomatology, Qingdao Municipal Hospital Affiliated to Qingdao
      University Medical College, Qingdao 266071, China.
FAU - Yixiang, Liao
AU  - Yixiang L
AD  - Center of Stomatology, Qingdao Municipal Hospital Affiliated to Qingdao
      University Medical College, Qingdao 266071, China.
FAU - Ning, Li
AU  - Ning L
AD  - Postgraduate School, Dalian Medical University, Dalian 116044, China.
FAU - Lei, Cao
AU  - Lei C
AD  - Postgraduate School, Dalian Medical University, Dalian 116044, China.
FAU - Zhenggang, Chen
AU  - Zhenggang C
AD  - Center of Stomatology, Qingdao Municipal Hospital Affiliated to Qingdao
      University Medical College, Qingdao 266071, China;Dept. of Oral and Maxillofacial
      Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School
      of Medicine, Shanghai 200011, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (CCND1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 124671-05-2 (RHOA protein, human)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - D
SB  - IM
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Cyclin D1
MH  - Gene Silencing
MH  - Humans
MH  - *Neoplasms, Squamous Cell
MH  - RNA, Messenger
MH  - RNA, Small Interfering
MH  - Real-Time Polymerase Chain Reaction
MH  - *Tongue Neoplasms
MH  - Transfection
MH  - rhoA GTP-Binding Protein
OAB - Publisher: RNARhoARhoA
      SCC-4RNARhoA3121RhoA-siRNA2RhoA-siRNANC-siRNAsiRNASCC-4RhoA mRNAWestern
      blotRhoACyclin D1p21p27 RhoAp21p27Cyclin D1P<0.05 RhoARhoARhoA.
OABL- eng
OTO - NOTNLM
OT  - *RNA interference
OT  - *RhoA
OT  - *cell proliferation
OT  - *tongue cancer
EDAT- 2017/03/21 06:00
MHDA- 2017/04/06 06:00
CRDT- 2017/03/21 06:00
AID - 10.7518/hxkq.2016.06.014 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Dec 1;34(6):620-625. doi:
      10.7518/hxkq.2016.06.014.

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