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Sodium Orthovanadate Inhibits Proliferation and Triggers Apoptosis in Oral Squamous Cell Carcinoma in vitro.

Abstract Sodium orthovanadate (SOV) is a general inhibitor of tyrosine phosphatases, a large family of enzymes that catalyze the removal of phosphate groups from tyrosine residues. SOV is commonly used in the laboratory to preserve the protein tyrosyl phosphorylation state of proteins under study. It has shown promising antineoplastic activity in some human cancer cell lines; this effect has not been fully investigated in head and neck squamous cell carcinoma. In this study, the effect of SOV on cell growth, proliferation, viability, and apoptosis was assessed in Cal27 cells, an oral squamous cell carcinoma (OSCC) cell line. SOV exhibited dose-dependent inhibition of cell growth and decrease in cell viability and colony formation. The IC50 values for treatment lasting 72 h and 7 days were 25 and 10 µM, respectively. The cytotoxic effect of the drug was associated with poly(ADP-ribose)polymerase cleavage detected by immunoblot. Flow cytometry of Cal27 cells stained with annexin V-FITC and propidium iodide showed a dose-dependent increase in apoptosis that reached approximately 40% at 25 µM SOV. These findings demonstrate that SOV has in vitro antiproliferative and proapoptotic effect on OSCC cells.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title biochemistry. biokhimiia
Publication Year Start




PMID- 28320298
OWN - NLM
STAT- MEDLINE
DA  - 20170321
DCOM- 20170322
LR  - 20170322
IS  - 1608-3040 (Electronic)
IS  - 0006-2979 (Linking)
VI  - 82
IP  - 2
DP  - 2017 Feb
TI  - Sodium Orthovanadate Inhibits Proliferation and Triggers Apoptosis in Oral
      Squamous Cell Carcinoma in vitro.
PG  - 149-155
LID - 10.1134/S0006297917020067 [doi]
AB  - Sodium orthovanadate (SOV) is a general inhibitor of tyrosine phosphatases, a
      large family of enzymes that catalyze the removal of phosphate groups from
      tyrosine residues. SOV is commonly used in the laboratory to preserve the protein
      tyrosyl phosphorylation state of proteins under study. It has shown promising
      antineoplastic activity in some human cancer cell lines; this effect has not been
      fully investigated in head and neck squamous cell carcinoma. In this study, the
      effect of SOV on cell growth, proliferation, viability, and apoptosis was
      assessed in Cal27 cells, an oral squamous cell carcinoma (OSCC) cell line. SOV
      exhibited dose-dependent inhibition of cell growth and decrease in cell viability
      and colony formation. The IC50 values for treatment lasting 72 h and 7 days were 
      25 and 10 microM, respectively. The cytotoxic effect of the drug was associated
      with poly(ADP-ribose)polymerase cleavage detected by immunoblot. Flow cytometry
      of Cal27 cells stained with annexin V-FITC and propidium iodide showed a
      dose-dependent increase in apoptosis that reached approximately 40% at 25 microM 
      SOV. These findings demonstrate that SOV has in vitro antiproliferative and
      proapoptotic effect on OSCC cells.
FAU - Khalil, A A
AU  - Khalil AA
AD  - University of Virginia Health System, Division of Head and Neck Oncologic and
      Microvascular Surgery, Department of Otolaryngology, Head and Neck Surgery,
      Virginia, USA. [email protected]
FAU - Jameson, M J
AU  - Jameson MJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry (Mosc)
JT  - Biochemistry. Biokhimiia
JID - 0376536
RN  - 0 (Neoplasm Proteins)
RN  - 3WHH0066W5 (Vanadates)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Squamous Cell/drug therapy/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Humans
MH  - Mouth Neoplasms/drug therapy/*metabolism/pathology
MH  - Neoplasm Proteins/metabolism
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Proteolysis/drug effects
MH  - Vanadates/*pharmacology
EDAT- 2017/03/23 06:00
MHDA- 2017/03/23 06:01
CRDT- 2017/03/22 06:00
AID - BCM82020258 [pii]
AID - 10.1134/S0006297917020067 [doi]
PST - ppublish
SO  - Biochemistry (Mosc). 2017 Feb;82(2):149-155. doi: 10.1134/S0006297917020067.

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