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Chronic Alcohol Intoxication Is Not Accompanied by an Increase in Calpain Proteolytic Activity in Cardiac Muscle of Rats.

Abstract Enzymatic activity of Ca2+-dependent calpain proteases as well as the content and gene expression of μ-calpain (activated by micromolar calcium ion concentrations), calpastatin (inhibitor of calpains), and titin (substrate for calpains) were investigated in cardiac muscles of rats subjected to chronic alcoholization for 3 and 6 months. There was no increase in the "heart weight/body weight" parameter indicating development of heart hypertrophy in the alcoholized rats, while a decreasing trend was observed for this parameter in the rats after 6-month modeling of alcoholic cardiomyopathy, which indicated development of atrophic changes in the myocardium. Fluorometric measurements conducted using the Calpain Activity Assay Kit did not reveal any changes in total calpain activity in protein extracts of cardiac muscles of the rats alcoholized for 3 and 6 months. Western blot analysis did not show reliable changes in the contents of μ-calpain and calpastatin, and SDS-PAGE did not reveal any decrease in the titin content in the myocardium of rats after the chronic alcohol intoxication. Autolysis of μ-calpain was also not verified, which could indicate that proteolytic activity of this enzyme in myocardium of chronically alcoholized rats is not enhanced. Using Pro-Q Diamond staining, changes in phosphorylation level of titin were not detected in cardiac muscle of rats after chronic alcoholization during three and six months. A decrease in µ-calpain and calpastatin mRNA content (~1.3-fold, p ≤ 0.01 and ~1.9-fold, p ≤ 0.01, respectively) in the myocardium of rats alcoholized for 3 months and decrease in calpastatin mRNA (~1.4-fold, p ≤ 0.01) in animals alcoholized for 6 months was demonstrated using real-time PCR. These results indicate negative effect of chronic alcohol intoxication on expression of the abovementioned genes.
PMID
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Authors

Mayor MeshTerms

Proteolysis

Keywords
Journal Title biochemistry. biokhimiia
Publication Year Start




PMID- 28320300
OWN - NLM
STAT- MEDLINE
DA  - 20170321
DCOM- 20170322
LR  - 20170322
IS  - 1608-3040 (Electronic)
IS  - 0006-2979 (Linking)
VI  - 82
IP  - 2
DP  - 2017 Feb
TI  - Chronic Alcohol Intoxication Is Not Accompanied by an Increase in Calpain
      Proteolytic Activity in Cardiac Muscle of Rats.
PG  - 168-175
LID - 10.1134/S0006297917020080 [doi]
AB  - Enzymatic activity of Ca2+-dependent calpain proteases as well as the content and
      gene expression of mu-calpain (activated by micromolar calcium ion
      concentrations), calpastatin (inhibitor of calpains), and titin (substrate for
      calpains) were investigated in cardiac muscles of rats subjected to chronic
      alcoholization for 3 and 6 months. There was no increase in the "heart
      weight/body weight" parameter indicating development of heart hypertrophy in the 
      alcoholized rats, while a decreasing trend was observed for this parameter in the
      rats after 6-month modeling of alcoholic cardiomyopathy, which indicated
      development of atrophic changes in the myocardium. Fluorometric measurements
      conducted using the Calpain Activity Assay Kit did not reveal any changes in
      total calpain activity in protein extracts of cardiac muscles of the rats
      alcoholized for 3 and 6 months. Western blot analysis did not show reliable
      changes in the contents of mu-calpain and calpastatin, and SDS-PAGE did not
      reveal any decrease in the titin content in the myocardium of rats after the
      chronic alcohol intoxication. Autolysis of mu-calpain was also not verified,
      which could indicate that proteolytic activity of this enzyme in myocardium of
      chronically alcoholized rats is not enhanced. Using Pro-Q Diamond staining,
      changes in phosphorylation level of titin were not detected in cardiac muscle of 
      rats after chronic alcoholization during three and six months. A decrease in
      micro-calpain and calpastatin mRNA content (~1.3-fold, p </= 0.01 and ~1.9-fold, 
      p </= 0.01, respectively) in the myocardium of rats alcoholized for 3 months and 
      decrease in calpastatin mRNA (~1.4-fold, p </= 0.01) in animals alcoholized for 6
      months was demonstrated using real-time PCR. These results indicate negative
      effect of chronic alcohol intoxication on expression of the abovementioned genes.
FAU - Gritsyna, Yu V
AU  - Gritsyna YV
AD  - Institute of Theoretical and Experimental Biophysics, Russian Academy of
      Sciences, Pushchino, Moscow Region, 142290, Russia. [email protected]
FAU - Salmov, N N
AU  - Salmov NN
FAU - Bobylev, A G
AU  - Bobylev AG
FAU - Fadeeva, I S
AU  - Fadeeva IS
FAU - Fesenko, N I
AU  - Fesenko NI
FAU - Sadikova, D G
AU  - Sadikova DG
FAU - Kukushkin, N I
AU  - Kukushkin NI
FAU - Podlubnaya, Z A
AU  - Podlubnaya ZA
FAU - Vikhlyantsev, I M
AU  - Vikhlyantsev IM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry (Mosc)
JT  - Biochemistry. Biokhimiia
JID - 0376536
RN  - 0 (Muscle Proteins)
RN  - EC 3.4.22.- (Calpain)
SB  - IM
MH  - Alcoholic Intoxication/*enzymology/pathology
MH  - Animals
MH  - Apoptosis
MH  - Calpain/*metabolism
MH  - Cardiomyopathy, Alcoholic/*enzymology/pathology
MH  - Chronic Disease
MH  - Male
MH  - Muscle Proteins/*metabolism
MH  - Myocardium/*enzymology/pathology
MH  - *Proteolysis
MH  - Rats
MH  - Rats, Wistar
EDAT- 2017/03/23 06:00
MHDA- 2017/03/23 06:01
CRDT- 2017/03/22 06:00
AID - BCM82020280 [pii]
AID - 10.1134/S0006297917020080 [doi]
PST - ppublish
SO  - Biochemistry (Mosc). 2017 Feb;82(2):168-175. doi: 10.1134/S0006297917020080.

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