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Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials.

Abstract Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals. Expression of the cloned H5 gene was achieved using the nisin-controlled gene expression system. Detection of the intracellular H5 antigen produced in L. lactis was performed by Western blot analysis and confirmed using mass spectrometry. The potential of L. lactis recombinant cells to induce an immune response was examined by setting up preliminary immunization trials on chickens and mice. Obtained sera were tested for specific antibodies by ELISA assays. The results of these studies are a promising step toward developing a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for efficient antigen production and delivery to the mucosal surface.
PMID
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Authors

Mayor MeshTerms

Hemagglutinin Glycoproteins, Influenza Virus

Influenza A Virus, H5N1 Subtype

Influenza Vaccines

Influenza in Birds

Lactococcus lactis

Keywords
Journal Title biomed research international
Publication Year Start




PMID- 28321412
OWN - NLM
STAT- MEDLINE
DA  - 20170321
DCOM- 20170322
LR  - 20170322
IS  - 2314-6141 (Electronic)
VI  - 2017
DP  - 2017
TI  - Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1
      Isolate and Its Immunological Effect in Preliminary Animal Trials.
PG  - 6747482
LID - 10.1155/2017/6747482 [doi]
AB  - Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that
      are gaining much interest as antigen producers for development of live vaccine
      vectors. Heterologous proteins of different origin have been successfully
      expressed in various LAB species, including Lactococcus lactis. Recombinant L.
      lactis strains have been shown to induce specific local and systemic immune
      responses against various antigens. Our study aimed at constructing a L. lactis
      strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and
      examining its effect on animals. Expression of the cloned H5 gene was achieved
      using the nisin-controlled gene expression system. Detection of the intracellular
      H5 antigen produced in L. lactis was performed by Western blot analysis and
      confirmed using mass spectrometry. The potential of L. lactis recombinant cells
      to induce an immune response was examined by setting up preliminary immunization 
      trials on chickens and mice. Obtained sera were tested for specific antibodies by
      ELISA assays. The results of these studies are a promising step toward developing
      a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for 
      efficient antigen production and delivery to the mucosal surface.
FAU - Szczepankowska, Agnieszka K
AU  - Szczepankowska AK
AUID- ORCID: 0000-0002-8733-3283
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw,
      Poland.
FAU - Szatraj, Katarzyna
AU  - Szatraj K
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw,
      Poland.
FAU - Salanski, Przemyslaw
AU  - Salanski P
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw,
      Poland.
FAU - Rozga, Agnieszka
AU  - Rozga A
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw,
      Poland.
FAU - Gorecki, Roman K
AU  - Gorecki RK
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw,
      Poland.
FAU - Bardowski, Jacek K
AU  - Bardowski JK
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw,
      Poland.
LA  - eng
PT  - Journal Article
DEP - 20170222
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Hemagglutinin Glycoproteins, Influenza Virus)
RN  - 0 (Influenza Vaccines)
RN  - 0 (hemagglutinin, human influenza A virus)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - *Hemagglutinin Glycoproteins, Influenza Virus/genetics/immunology
MH  - *Influenza A Virus, H5N1 Subtype/genetics/immunology
MH  - *Influenza Vaccines/genetics/immunology
MH  - *Influenza in Birds/immunology/prevention & control
MH  - *Lactococcus lactis/genetics/immunology
MH  - Mice
PMC - PMC5340954
COI - The authors declare that there are no competing interests regarding the
      publication of this paper.
EDAT- 2017/03/23 06:00
MHDA- 2017/03/23 06:01
CRDT- 2017/03/22 06:00
PHST- 2016/12/19 [received]
PHST- 2017/01/20 [revised]
PHST- 2017/01/24 [accepted]
AID - 10.1155/2017/6747482 [doi]
PST - ppublish
SO  - Biomed Res Int. 2017;2017:6747482. doi: 10.1155/2017/6747482. Epub 2017 Feb 22.

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