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PMID- 28326728
OWN - NLM
STAT- MEDLINE
DA  - 20170322
DCOM- 20170412
LR  - 20170412
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 35
IP  - 1
DP  - 2017 Feb 01
TI  - [Effect of clock gene PER1 knockdown on clock gene networks in human oral
      squamous cell carcinoma].
PG  - 57-62
LID - 10.7518/hxkq.2017.01.008 [doi]
AB  - OBJECTIVE: This study investigated the effect of clock gene PER1 on the
      expression levels of other clock genes in clock gene networks in oral squamous
      cell carcinoma cells. METHODS: We used RNA interference mediated by short hairpin
      RNAs (shRNAs) to effectively knock down PER1 in SCC15 human oral squamous cell
      carcinoma cells. Flow cytometry was used to detect the degree of proliferation
      and apoptosis of the cells after PER1 knockdown, and quantitative real-time PCR
      was used to detect the mRNA expression levels of the clock genes CLOCK, BMAL1,
      PER1, PER2, PER3, DEC1, DEC2, CRY1, CRY2, TIM, CKIE, RORA, NPAS2, and REV-ERBA.
      RESULTS: The proliferation index of SCC15 cells increased significantly while the
      apoptotic index decreased significantly after PER1 knockdown (P<0.05). The mRNA
      expression levels of PER1, PER2, DEC1, DEC2, CRY1, CRY2, and NPAS2 markedly
      decreased (P<0.05) while those of PER3, TIM, RORA, and REV-ERBA markedly
      increased (P<0.05). By contrast, no obvious changes were observed in the mRNA
      expression levels of CLOCK, BMAL1, and CKIE (P>0.05). CONCLUSIONS: The clock gene
      PER1 can regulate the expression levels of other clock genes in the clock gene
      networks; these genes include PER2, DEC1, DEC2, CRY1, CRY2, NPAS2, PER3, TIM,
      RORA, and REV-ERBA. PER1 gene thus plays an important role in the regulation of
      clock gene networks..
FAU - Qin, Zhao
AU  - Qin Z
AD  - Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of
      Chongqing Medical University, Chongqing 400016, China.
FAU - Yiran, Ao
AU  - Yiran A
AD  - Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of
      Chongqing Medical University, Chongqing 400016, China.
FAU - Kai, Yang
AU  - Kai Y
AD  - Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of
      Chongqing Medical University, Chongqing 400016, China.
FAU - Xiaoli, Su
AU  - Xiaoli S
AD  - Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of
      Chongqing Medical University, Chongqing 400016, China.
FAU - Xiaoqiang, Lu
AU  - Xiaoqiang L
AD  - Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of
      Chongqing Medical University, Chongqing 400016, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (RNA, Small Interfering)
RN  - Carcinoma, squamous cell of head and neck
SB  - D
SB  - IM
MH  - Apoptosis
MH  - *Carcinoma, Squamous Cell
MH  - *Gene Regulatory Networks
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - *Mouth Neoplasms
MH  - RNA Interference
MH  - RNA, Small Interfering
MH  - Real-Time Polymerase Chain Reaction
OAB - Publisher: PER1
      RNASCC15PER1CLOCKBMAL1PER1PER2PER3DEC1DEC2CRY1CRY2TIMCKIERORANPAS2REV-ERBA mRNA
      PER1SCC15P<0.05PER1PER2DEC1DEC2CRY1CRY2NPAS2 mRNAP<0.05PER3TIMRORAREV-ERBA
      mRNAP<0.05CLOCKBMAL1CKIE mRNAP>0.05
      PER1PER2DEC1DEC2CRY1CRY2NPAS2PER3TIMRORAREV-ERBAPER1.
OABL- eng
OTO - NOTNLM
OT  - *circadian clock
OT  - *gene
OT  - *oral cavity
OT  - *squamous cell carcinoma
EDAT- 2017/03/23 06:00
MHDA- 2017/04/13 06:00
CRDT- 2017/03/23 06:00
AID - 10.7518/hxkq.2017.01.008 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Feb 1;35(1):57-62. doi:
      10.7518/hxkq.2017.01.008.

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