PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 28373443
OWN - NLM
STAT- MEDLINE
DA  - 20170404
DCOM- 20170413
LR  - 20170413
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 37
IP  - 4
DP  - 2017 Apr
TI  - Dynamic Phenotypic Transition of Breast Cancer Cells In Vitro Revealed by
      Self-floating Cell Culture.
PG  - 1793-1797
AB  - BACKGROUND: Breast tumor heterogeneity leads to phenotypic diversity, such as
      tumor-initiating and metastatic properties and drug sensitivity. MATERIALS AND
      METHODS: We found that a self-floating cell (SFC) culture enriches a
      drug-resistant subpopulation in a HER2-positive breast cancer cell line. SFCs
      were analyzed for cancer stem cell markers, gene expression profiles, and
      sensitivity for anticancer drugs. RESULTS: SFCs expressed cancer stem cell
      markers, such as aldehyde dehydrogenase (ALDH) activity and elevated HER2
      autophosphorylation. Gene expression profiles of SFCs showed a dramatic
      difference compared to those of parental or forced floating cells. SFCs also
      expressed CD133, a marker of drug resistance, and resisted cytotoxic drugs by
      drug efflux transporters. In contrast, HER2 kinase inhibitors efficiently reduced
      SFC viability. CONCLUSION: SFCs enrich drug-resistant subpopulations even in
      vitro and might reflect the highly plastic nature of breast cancer cells even in 
      vitro.
CI  - Copyright(c) 2017, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Oku, Yusuke
AU  - Oku Y
AD  - Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical 
      University School of Pharmacy, Iwate, Japan [email protected]
FAU - Nishiya, Naoyuki
AU  - Nishiya N
AD  - Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical 
      University School of Pharmacy, Iwate, Japan.
FAU - Tsuda, Kayoko
AU  - Tsuda K
AD  - Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical 
      University School of Pharmacy, Iwate, Japan.
FAU - Shibazaki, Masahiko
AU  - Shibazaki M
AD  - Department of Tumor Biology, Institute of Biomedical Science, Iwate Medical
      University, Iwate, Japan.
FAU - Maesawa, Chihaya
AU  - Maesawa C
AD  - Department of Tumor Biology, Institute of Biomedical Science, Iwate Medical
      University, Iwate, Japan.
FAU - Uehara, Yoshimasa
AU  - Uehara Y
AD  - Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical 
      University School of Pharmacy, Iwate, Japan.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biomarkers, Tumor)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
SB  - IM
MH  - Adenocarcinoma/drug therapy/genetics/metabolism/*pathology
MH  - Adenocarcinoma, Papillary/drug therapy/genetics/metabolism/*pathology
MH  - Aldehyde Dehydrogenase/genetics/metabolism
MH  - Antineoplastic Agents/pharmacology
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Blotting, Western
MH  - Breast Neoplasms/drug therapy/genetics/metabolism/*pathology
MH  - Cell Culture Techniques/*methods
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Drug Resistance, Neoplasm/genetics
MH  - Female
MH  - Flow Cytometry
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - In Vitro Techniques
MH  - Neoplastic Stem Cells/drug effects/metabolism/*pathology
MH  - Phenotype
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - *HER2
OT  - *Tumor heterogeneity
OT  - *breast cancer
OT  - *drug resistance
EDAT- 2017/04/05 06:00
MHDA- 2017/04/14 06:00
CRDT- 2017/04/05 06:00
PHST- 2017/01/27 [received]
PHST- 2017/03/13 [revised]
PHST- 2017/03/14 [accepted]
AID - 37/4/1793 [pii]
AID - 10.21873/anticanres.11513 [doi]
PST - ppublish
SO  - Anticancer Res. 2017 Apr;37(4):1793-1797.

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