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Triptolide inhibits the growth of osteosarcoma by regulating microRNA-181a via targeting PTEN gene in vivo and vitro.

Abstract We aimed to study the anti-tumor effects of triptolide on osteosarcoma and the related molecular mechanisms. The cell viability, apoptosis portion, tumor size, tumor weight, and invasion of osteosarcoma cells were determined. The relative level of microRNA-181 in osteosarcoma tissues and the adjacent tissues was determined by quantitative real-time reverse transcription polymerase chain reaction. The target gene of microRNA-181a was determined and verified by luciferase report assay. At last, osteosarcoma cells were treated with triptolide and triptolide + microRNA-181a mimics to verify the relationship between triptolide and microRNA-181a. Triptolide inhibited the cell viability, promoted the apoptosis, decreased the tumor size and weight, and reduced the invasion of osteosarcoma cells. The level of microRNA-181a in osteosarcoma cells decreased significantly after treating with triptolide, and the relative level of microRNA-181a in osteosarcoma tissues was markedly higher than that in the adjacent tissues. PTEN was reported and verified the direct target gene of microRNA-181a. The overexpression of microRNA-181a decreased the inhibition of triptolide on osteosarcoma proliferation and promotion on osteosarcoma apoptosis. In conclusion, triptolide inhibited cell growth and invasion of osteosarcoma by regulating microRNA-181a via targeting PTEN gene in vivo and vitro.
PMID
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Authors

Mayor MeshTerms
Keywords

PTEN

Triptolide

miR-181a

osteosarcoma

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28381158
OWN - NLM
STAT- MEDLINE
DA  - 20170406
DCOM- 20170418
LR  - 20170418
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 4
DP  - 2017 Apr
TI  - Triptolide inhibits the growth of osteosarcoma by regulating microRNA-181a via
      targeting PTEN gene in vivo and vitro.
PG  - 1010428317697556
LID - 10.1177/1010428317697556 [doi]
AB  - We aimed to study the anti-tumor effects of triptolide on osteosarcoma and the
      related molecular mechanisms. The cell viability, apoptosis portion, tumor size, 
      tumor weight, and invasion of osteosarcoma cells were determined. The relative
      level of microRNA-181 in osteosarcoma tissues and the adjacent tissues was
      determined by quantitative real-time reverse transcription polymerase chain
      reaction. The target gene of microRNA-181a was determined and verified by
      luciferase report assay. At last, osteosarcoma cells were treated with triptolide
      and triptolide + microRNA-181a mimics to verify the relationship between
      triptolide and microRNA-181a. Triptolide inhibited the cell viability, promoted
      the apoptosis, decreased the tumor size and weight, and reduced the invasion of
      osteosarcoma cells. The level of microRNA-181a in osteosarcoma cells decreased
      significantly after treating with triptolide, and the relative level of
      microRNA-181a in osteosarcoma tissues was markedly higher than that in the
      adjacent tissues. PTEN was reported and verified the direct target gene of
      microRNA-181a. The overexpression of microRNA-181a decreased the inhibition of
      triptolide on osteosarcoma proliferation and promotion on osteosarcoma apoptosis.
      In conclusion, triptolide inhibited cell growth and invasion of osteosarcoma by
      regulating microRNA-181a via targeting PTEN gene in vivo and vitro.
FAU - Jiang, Chunming
AU  - Jiang C
AD  - 1 Department of Pediatrics, Hangzhou First People's Hospital, Nanjing Medical
      University, Hangzhou, China.
FAU - Fang, Xiang
AU  - Fang X
AD  - 2 Department of Clinical Laboratory, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
FAU - Zhang, Hongxu
AU  - Zhang H
AD  - 3 Department of Ophthalmology, Hangzhou First People's Hospital, Nanjing Medical 
      University, Hangzhou, China.
FAU - Wang, Xuepeng
AU  - Wang X
AD  - 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
AD  - 5 Hangzhou Orthopedic Institute, Hangzhou, China.
FAU - Li, Maoqiang
AU  - Li M
AD  - 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
AD  - 5 Hangzhou Orthopedic Institute, Hangzhou, China.
FAU - Jiang, Wu
AU  - Jiang W
AD  - 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
AD  - 5 Hangzhou Orthopedic Institute, Hangzhou, China.
FAU - Tian, Fei
AU  - Tian F
AD  - 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
AD  - 5 Hangzhou Orthopedic Institute, Hangzhou, China.
FAU - Zhu, Liulong
AU  - Zhu L
AD  - 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
AD  - 5 Hangzhou Orthopedic Institute, Hangzhou, China.
FAU - Bian, Zhenyu
AU  - Bian Z
AD  - 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing
      Medical University, Hangzhou, China.
AD  - 5 Hangzhou Orthopedic Institute, Hangzhou, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (Antineoplastic Agents, Alkylating)
RN  - 0 (Diterpenes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (MIrn181 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Phenanthrenes)
RN  - 19ALD1S53J (triptolide)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Antineoplastic Agents, Alkylating/*pharmacology
MH  - Apoptosis/drug effects
MH  - Bone Neoplasms/*drug therapy/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Diterpenes/*pharmacology
MH  - Epithelial-Mesenchymal Transition/drug effects
MH  - Epoxy Compounds/pharmacology
MH  - Humans
MH  - MicroRNAs/antagonists & inhibitors/*physiology
MH  - Neoplasm Invasiveness
MH  - Osteosarcoma/*drug therapy/pathology
MH  - PTEN Phosphohydrolase/*genetics
MH  - Phenanthrenes/*pharmacology
OTO - NOTNLM
OT  - PTEN
OT  - Triptolide
OT  - miR-181a
OT  - osteosarcoma
EDAT- 2017/04/07 06:00
MHDA- 2017/04/19 06:00
CRDT- 2017/04/07 06:00
AID - 10.1177/1010428317697556 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Apr;39(4):1010428317697556. doi: 10.1177/1010428317697556.

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