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The apoptotic and genomic studies on A549 cell line induced by silver nitrate.

Abstract Lung cancer is the leading cause of male cancer deaths worldwide. Metal-based anticancer drugs have evolved significantly during the past decades. Recently, silver ions have been investigated for their anticancer effects. We aimed to study the time-course cytotoxic effects of silver nitrate on A549 adenocarcinomic human alveolar basal epithelial cells to provide insights into the molecular-level understanding of growth suppression mechanism involved in apoptosis. The influences of silver nitrate were studied via MTT assay, flow cytometry, immunocytochemical, confocal and transmission electron microscopy, and microarray assays. Silver nitrate showed inhibitory effects against A549 cells in a dose- and time-dependent manner for 24, 48, and 72 h and induced apoptosis. The early and late apoptotic cells and depolarized mitochondrial membrane potential were determined by the half-maximal inhibitory concentration (IC50) value of silver nitrate treated for 72 h. But cysteinyl aspartate proteinase-3 was not activated for 72 h. Furthermore, IC50 value of silver nitrate also induced apoptosis according to immunocytochemical assays for 72 h. The downregulated CCNY, HNRNPL, ASF1B, PIAS4, HNRNPH1, EIF2C2, TAF15, FOXC1, LEP, and PCB2 genes administered with silver nitrate IC50 were identified as apoptosis-leading genes. Silver nitrate may be a suitable therapeutic agent against lung cancer.
PMID
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Authors

Mayor MeshTerms
Keywords

A549

Silver nitrate

apoptosis

flow cytometry

immunocytochemistry

microarray

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28381165
OWN - NLM
STAT- MEDLINE
DA  - 20170406
DCOM- 20170418
LR  - 20170418
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 4
DP  - 2017 Apr
TI  - The apoptotic and genomic studies on A549 cell line induced by silver nitrate.
PG  - 1010428317695033
LID - 10.1177/1010428317695033 [doi]
AB  - Lung cancer is the leading cause of male cancer deaths worldwide. Metal-based
      anticancer drugs have evolved significantly during the past decades. Recently,
      silver ions have been investigated for their anticancer effects. We aimed to
      study the time-course cytotoxic effects of silver nitrate on A549 adenocarcinomic
      human alveolar basal epithelial cells to provide insights into the
      molecular-level understanding of growth suppression mechanism involved in
      apoptosis. The influences of silver nitrate were studied via MTT assay, flow
      cytometry, immunocytochemical, confocal and transmission electron microscopy, and
      microarray assays. Silver nitrate showed inhibitory effects against A549 cells in
      a dose- and time-dependent manner for 24, 48, and 72 h and induced apoptosis. The
      early and late apoptotic cells and depolarized mitochondrial membrane potential
      were determined by the half-maximal inhibitory concentration (IC50) value of
      silver nitrate treated for 72 h. But cysteinyl aspartate proteinase-3 was not
      activated for 72 h. Furthermore, IC50 value of silver nitrate also induced
      apoptosis according to immunocytochemical assays for 72 h. The downregulated
      CCNY, HNRNPL, ASF1B, PIAS4, HNRNPH1, EIF2C2, TAF15, FOXC1, LEP, and PCB2 genes
      administered with silver nitrate IC50 were identified as apoptosis-leading genes.
      Silver nitrate may be a suitable therapeutic agent against lung cancer.
FAU - Kaplan, Ayse
AU  - Kaplan A
AD  - 1 Faculty of Science, Department of Biology, Anadolu University, Eskisehir,
      Turkey.
FAU - Akalin Ciftci, Gulsen
AU  - Akalin Ciftci G
AD  - 2 Faculty of Pharmacy, Department of Biochemistry, Anadolu University, Eskisehir,
      Turkey.
FAU - Kutlu, Hatice Mehtap
AU  - Kutlu HM
AD  - 1 Faculty of Science, Department of Biology, Anadolu University, Eskisehir,
      Turkey.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 95IT3W8JZE (Silver Nitrate)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - A549 Cells
MH  - Apoptosis/*drug effects
MH  - Caspase 3/metabolism
MH  - Genomics
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung Neoplasms/genetics/*pathology/ultrastructure
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Microscopy, Electron, Transmission
MH  - Oligonucleotide Array Sequence Analysis
MH  - Silver Nitrate/*pharmacology
OTO - NOTNLM
OT  - A549
OT  - Silver nitrate
OT  - apoptosis
OT  - flow cytometry
OT  - immunocytochemistry
OT  - microarray
EDAT- 2017/04/07 06:00
MHDA- 2017/04/19 06:00
CRDT- 2017/04/07 06:00
AID - 10.1177/1010428317695033 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Apr;39(4):1010428317695033. doi: 10.1177/1010428317695033.

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