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Identification and validation of a novel autophagy gene expression signature for human bladder cancer patients.

Abstract We sought to identify and validate a novel urinary autophagy transcript signature in patients with bladder cancer and evaluate its clinical utility. We performed an initial screening for seven autophagy transcript-based panel (autophagy-related protein 12 (ATG12); WD repeat domain, phosphoinositide interacting 2 (WIPI2); FYVE and coiled-coil domain-containing protein 1 (FYCO1); microtubule-associated protein light chain (MAPLC3); RB1-inducible coiled-coil 1 (RB1CC1); tachylectin-II-like beta-propeller domain 1 (TECPR1); and Unc-51-like kinase (ULK1)) that was identified based on bioinformatics analysis followed by SYBR Green-based polymerase chain reaction array validation in paired tissue and urine samples. Afterward, we evaluated the expression of differentially expressed autophagy transcripts in an independent validation set with reverse transcription quantitative real-time polymerase chain reaction in urine sediments of 140 patients with bladder cancer, 68 patients with benign urological lesions, and 74 healthy controls (age and sex matched). The expression levels of ATG12, FYCO1, TECPR1, and ULK1 in paired bladder tissue and urine samples were significantly lower in bladder cancer than in control group (pā€‰<ā€‰0.001). In the validation set, the receiver-operating characteristic curve analyses demonstrated that each urinary autophagy transcripts showed high sensitivity and specificity for distinguishing bladder cancer from non-bladder cancer patients (ATG12, 75.4% and 86.1%; FYCO1, 87% and 75.7%; ULK1, 85.5% and 75.6%; and TECPR1, 90% and 81.9%). We document and validate a novel autophagy transcript signature for human bladder cancer diagnosis: bilharzial and non-bilharzial types.
PMID
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Authors

Mayor MeshTerms
Keywords

Bladder cancer

autophagy

bilharziasis

biomarkers

urine cytology

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28381171
OWN - NLM
STAT- MEDLINE
DA  - 20170406
DCOM- 20170418
LR  - 20170418
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 4
DP  - 2017 Apr
TI  - Identification and validation of a novel autophagy gene expression signature for 
      human bladder cancer patients.
PG  - 1010428317698360
LID - 10.1177/1010428317698360 [doi]
AB  - We sought to identify and validate a novel urinary autophagy transcript signature
      in patients with bladder cancer and evaluate its clinical utility. We performed
      an initial screening for seven autophagy transcript-based panel
      (autophagy-related protein 12 (ATG12); WD repeat domain, phosphoinositide
      interacting 2 (WIPI2); FYVE and coiled-coil domain-containing protein 1 (FYCO1); 
      microtubule-associated protein light chain (MAPLC3); RB1-inducible coiled-coil 1 
      (RB1CC1); tachylectin-II-like beta-propeller domain 1 (TECPR1); and Unc-51-like
      kinase (ULK1)) that was identified based on bioinformatics analysis followed by
      SYBR Green-based polymerase chain reaction array validation in paired tissue and 
      urine samples. Afterward, we evaluated the expression of differentially expressed
      autophagy transcripts in an independent validation set with reverse transcription
      quantitative real-time polymerase chain reaction in urine sediments of 140
      patients with bladder cancer, 68 patients with benign urological lesions, and 74 
      healthy controls (age and sex matched). The expression levels of ATG12, FYCO1,
      TECPR1, and ULK1 in paired bladder tissue and urine samples were significantly
      lower in bladder cancer than in control group (p &lt; 0.001). In the validation set,
      the receiver-operating characteristic curve analyses demonstrated that each
      urinary autophagy transcripts showed high sensitivity and specificity for
      distinguishing bladder cancer from non-bladder cancer patients (ATG12, 75.4% and 
      86.1%; FYCO1, 87% and 75.7%; ULK1, 85.5% and 75.6%; and TECPR1, 90% and 81.9%).
      We document and validate a novel autophagy transcript signature for human bladder
      cancer diagnosis: bilharzial and non-bilharzial types.
FAU - Eissa, Sanaa
AU  - Eissa S
AD  - 1 Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology
      Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
FAU - Matboli, Marwa
AU  - Matboli M
AD  - 1 Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology
      Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
FAU - Awad, Nahla
AU  - Awad N
AD  - 2 Early Cancer Detection Unit, Pathology Department, Faculty of Medicine, Ain
      Shams University, Cairo, Egypt.
FAU - Kotb, Yousif
AU  - Kotb Y
AD  - 3 Urology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
PT  - Validation Studies
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (ATG12 protein, human)
RN  - 0 (Autophagy-Related Protein 12)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FYCO1 protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (TECPR1 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog)
RN  - EC 2.7.11.1 (ULK1 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Autophagy/*genetics
MH  - Autophagy-Related Protein 12/genetics
MH  - Autophagy-Related Protein-1 Homolog/genetics
MH  - Biomarkers, Tumor
MH  - Computational Biology
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/genetics
MH  - Male
MH  - Membrane Proteins/genetics
MH  - Middle Aged
MH  - Transcription Factors/genetics
MH  - Transcriptome
MH  - Urinary Bladder/metabolism
MH  - Urinary Bladder Neoplasms/*diagnosis/metabolism
OTO - NOTNLM
OT  - *Bladder cancer
OT  - *autophagy
OT  - *bilharziasis
OT  - *biomarkers
OT  - *urine cytology
EDAT- 2017/04/07 06:00
MHDA- 2017/04/19 06:00
CRDT- 2017/04/07 06:00
AID - 10.1177/1010428317698360 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Apr;39(4):1010428317698360. doi: 10.1177/1010428317698360.

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