PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.

Abstract Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800-10000 Da were analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and 10 TPE were included to verify the model. We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method. Results. Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test. The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P = 0.035). Conclusions. The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.
PMID
Related Publications

Diagnostic value of interleukin 21 and carcinoembryonic antigen levels in malignant pleural effusions.

Detection of lung cancer using plasma protein profiling by matrix-assisted laser desorption/ionization mass spectrometry.

Proteomic study of benign and malignant pleural effusion.

Detection of lung adenocarcinoma using magnetic beads based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry serum protein profiling.

Preliminary study of MALDI-TOF mass spectrometry-based screening of patients with the NSCLC serum-specific peptides.

Authors

Mayor MeshTerms
Keywords
Journal Title disease markers
Publication Year Start




PMID- 28386154
OWN - NLM
STAT- MEDLINE
DA  - 20170407
DCOM- 20170420
LR  - 20170420
IS  - 1875-8630 (Electronic)
IS  - 0278-0240 (Linking)
VI  - 2017
DP  - 2017
TI  - The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung
      Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass
      Spectrometry.
PG  - 3160426
LID - 10.1155/2017/3160426 [doi]
AB  - Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem
      in clinics. In our study, we compared the peptide profiles of MPE and
      tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted
      laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in
      diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly
      assigned to training set and validation set. Peptides were isolated by weak
      cation exchange magnetic beads and peaks in the m/z range of 800-10000 Da were
      analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in
      training set by ClinProTools software, we screened the specific biomarkers and
      established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and
      10 TPE were included to verify the model. We additionally determined
      carcinoembryonic antigen (CEA) in MPE and TPE samples using
      electrochemiluminescent immunoassay method. Results. Five peptide peaks (917.37
      Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate 
      MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the
      classification were 93.75%, 100%, and 96.15%, respectively, after blinded test.
      The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification
      (P = 0.035). Conclusions. The results indicate MALDI-TOF-MS is a potential method
      for diagnosing MPE.
FAU - Xu, Jing
AU  - Xu J
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Xu, Bin
AU  - Xu B
AD  - National Center of Biomedical Analysis, Beijing, China.
FAU - Tang, Chuanhao
AU  - Tang C
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Li, Xiaoyan
AU  - Li X
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Qin, Haifeng
AU  - Qin H
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Wang, Weixia
AU  - Wang W
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Wang, Hong
AU  - Wang H
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Wang, Zhongyuan
AU  - Wang Z
AD  - Department of Tuberculosis, 309 Hospital, PLA, Beijing, China.
FAU - Li, Liangliang
AU  - Li L
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - Li, Zhihua
AU  - Li Z
AD  - Department of Oncology, General Hospital of the PLA Rocket Force, Beijing, China.
FAU - Gao, Hongjun
AU  - Gao H
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
FAU - He, Kun
AU  - He K
AUID- ORCID: 0000-0001-6706-8649
AD  - National Center of Biomedical Analysis, Beijing, China.
FAU - Liu, Xiaoqing
AU  - Liu X
AUID- ORCID: 0000-0001-7738-109X
AD  - Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20170313
PL  - United States
TA  - Dis Markers
JT  - Disease markers
JID - 8604127
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Peptides)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoembryonic Antigen/*metabolism
MH  - Case-Control Studies
MH  - Early Detection of Cancer/methods
MH  - Female
MH  - Humans
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Peptides/*metabolism
MH  - Pleural Effusion, Malignant/*metabolism/pathology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
PMC - PMC5366757
EDAT- 2017/04/08 06:00
MHDA- 2017/04/21 06:00
CRDT- 2017/04/08 06:00
PHST- 2016/11/30 [received]
PHST- 2017/02/15 [accepted]
AID - 10.1155/2017/3160426 [doi]
PST - ppublish
SO  - Dis Markers. 2017;2017:3160426. doi: 10.1155/2017/3160426. Epub 2017 Mar 13.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>