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Variation in fat content between liver lobes and comparison with histopathological scores in dairy cows with fatty liver.

Abstract The assessment of a liver biopsy remains the gold standard for diagnosing and staging fatty liver in dairy cows, which is often necessary for diagnostic and research purposes. Accuracy of the diagnosis relays on the quality of the biopsy, the assumed representativeness of a small tissue sample for a disease process throughout the liver and accurate human evaluation of histologic specimens. The objective of the present study was to assess the distribution of triacylglycerol (TAG) infiltration throughout the parenchyma of livers with different degrees of fatty liver in dairy cows. In addition, histopathological scores from the corresponding specimens were compared to a quantitative measurement of TAG, as well as the agreement between two observers.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title bmc veterinary research
Publication Year Start




PMID- 28403840
OWN - NLM
STAT- MEDLINE
DA  - 20170413
DCOM- 20170418
LR  - 20170418
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 13
IP  - 1
DP  - 2017 Apr 12
TI  - Variation in fat content between liver lobes and comparison with
      histopathological scores in dairy cows with fatty liver.
PG  - 98
LID - 10.1186/s12917-017-1004-9 [doi]
AB  - BACKGROUND: The assessment of a liver biopsy remains the gold standard for
      diagnosing and staging fatty liver in dairy cows, which is often necessary for
      diagnostic and research purposes. Accuracy of the diagnosis relays on the quality
      of the biopsy, the assumed representativeness of a small tissue sample for a
      disease process throughout the liver and accurate human evaluation of histologic 
      specimens. The objective of the present study was to assess the distribution of
      triacylglycerol (TAG) infiltration throughout the parenchyma of livers with
      different degrees of fatty liver in dairy cows. In addition, histopathological
      scores from the corresponding specimens were compared to a quantitative
      measurement of TAG, as well as the agreement between two observers. METHODS:
      Thirty livers with different degrees of lipid infiltration were selected and 10
      different locations throughout the liver were assessed. The TAG content was
      measured enzymatically, calculated in % or mg/g wet weight, and assigned to a
      scoring system. Corresponding tissue specimens were stained with
      hematoxylin-eosin (H&E) and Oil red O (ORO) for histopathological evaluation,
      using a scoring system. RESULTS: The difference in TAG content between any
      locations was less than 2%. Based on the scoring system the TAG concentration was
      even distributed in 79.3% of the livers. Based on kappa statistics the agreement 
      between two pathologists and staining technique in scoring histological specimens
      was moderate to fair. CONCLUSIONS: Overall the distribution of TAG throughout the
      liver and the accuracy of human evaluation of liver biopsies may lead to
      acceptable diagnoses for clinical purposes. Within the liver lobules a common
      pattern of lipid distribution depending on severity could be observed. For the
      staging of lipid infiltration for research projects, some degree of variation
      needs to be considered.
FAU - Gerspach, C
AU  - Gerspach C
AD  - Department of Farm Animals, Vetsuisse Faculty, University of Zurich,
      Winterthurerstrasse 260, Zurich, Switzerland. [email protected]
FAU - Imhasly, S
AU  - Imhasly S
AD  - Institute of Pharmacology and Toxicology, Vetsuisse Faculty, University of
      Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland.
FAU - Klingler, R
AU  - Klingler R
AD  - Department of Farm Animals, Vetsuisse Faculty, University of Zurich,
      Winterthurerstrasse 260, Zurich, Switzerland.
FAU - Hilbe, M
AU  - Hilbe M
AD  - Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich,
      Winterthurerstrasse 268, Zurich, Switzerland.
FAU - Hartnack, S
AU  - Hartnack S
AD  - Section of Epidemiology, Vetsuisse Faculty, University of Zurich,
      Winterthurerstrasse 270, Zurich, Switzerland.
FAU - Ruetten, M
AU  - Ruetten M
AD  - Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich,
      Winterthurerstrasse 268, Zurich, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20170412
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Biopsy/standards/*veterinary
MH  - Cattle
MH  - Cattle Diseases/diagnosis/*pathology
MH  - Fatty Liver/diagnosis/pathology/*veterinary
MH  - Female
MH  - Lipids/*analysis
MH  - Liver/*chemistry/*pathology
PMC - PMC5389092
EDAT- 2017/04/14 06:00
MHDA- 2017/04/19 06:00
CRDT- 2017/04/14 06:00
PHST- 2015/08/06 [received]
PHST- 2017/03/28 [accepted]
AID - 10.1186/s12917-017-1004-9 [doi]
AID - 10.1186/s12917-017-1004-9 [pii]
PST - epublish
SO  - BMC Vet Res. 2017 Apr 12;13(1):98. doi: 10.1186/s12917-017-1004-9.

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