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Anticholangiocarcinoma activity and toxicity of the Kaempferia galanga Linn. Rhizome ethanolic extract.

Abstract Cholangiocarcinoma (CCA) is an important public health problem in several tropical and subtropical parts of the world particularly Thailand. Chemotherapy of CCA is largely ineffective and discovery and development of effective alternative drugs is urgently needed. The objective of the study was to confirm the anti-CCA potential as well as toxicity of the crude extract of Kaempferia galangal Linn. (rhizome) both in vitro and in animal models.
PMID
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Authors

Mayor MeshTerms
Keywords

Anti-CCA

CL-6

Cholangiocarcinoma

Cytoxicity

Ethyl-p-methoxycinnamate (EPMC)

Kaempferia galangal Linn

Nude mouse xenograft model

Journal Title bmc complementary and alternative medicine
Publication Year Start




PMID- 28403856
OWN - NLM
STAT- MEDLINE
DA  - 20170413
DCOM- 20170420
LR  - 20170420
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Apr 12
TI  - Anticholangiocarcinoma activity and toxicity of the Kaempferia galanga Linn.
      Rhizome ethanolic extract.
PG  - 213
LID - 10.1186/s12906-017-1713-4 [doi]
AB  - BACKGROUND: Cholangiocarcinoma (CCA) is an important public health problem in
      several tropical and subtropical parts of the world particularly Thailand.
      Chemotherapy of CCA is largely ineffective and discovery and development of
      effective alternative drugs is urgently needed. The objective of the study was to
      confirm the anti-CCA potential as well as toxicity of the crude extract of
      Kaempferia galangal Linn. (rhizome) both in vitro and in animal models. METHODS: 
      The ethanolic extract of K. galanga Linn. rhizome, ethyl-p-methoxycinnamate
      (EPMC) and 5-fluorouracil (5-FU) were evaluated for their cytotoxic activities
      against CCA cell line (CL-6) using MTT cell proliferation assay. Acute and
      subacute toxicity of the extract were evaluated in ICR (Imprinting Control
      Region) mice according to the OECD (International Organization for Economic
      Co-operation and Development) Guideline. Anti-CCA activity was evaluated in CCA- 
      xenografted nude mice. RESULTS: Results of cytotoxicity test showed moderate
      activity of the extract and EPMC with median (95% confidence interval: 95% CI)
      50% inhibitory concentration (IC50) of 64.2 (57.76-72.11) and 49.19 (48.16-52.29)
      mug/ml, respectively. The IC50 of 5-FU was 107.1 (103.53-109.64) mug/ml. The
      selectivity index (SI) values for the extract, EPMC and 5-FU against human normal
      cell line (OUMS) and cancer cell line (CL-6) were 2.2, 2.09 and 1.31,
      respectively. Toxicity testing revealed no overt toxic effect up to the maximum
      single oral dose of 5000 mg/kg body weight and up to daily dose of 1000 mg/kg
      body weight for 30 days. The extract at the maximum tolerated dose level of 1000 
      mg/kg body weight for 30 days exhibited promising anti-CCA activity in
      CL6-xenografted nude mice as determined by inhibitory activity on tumor growth
      (58.41%) and lung metastasis (33.3%), as well as prolongation of survival time
      (62 days). CONCLUSION: The K. galangal Linn. rhizome extract and its bioactive
      compound EPMC exhibited moderate cytotoxic activity against human CCA tumor
      (CL-6) cell line. Results of toxicity testing suggest that the extract was well
      tolerated up to the maximum single oral dose of 5000 mg/kg body weight and daily 
      dose of 1000 mg/kg body weight for 30 days. The extract exhibited promising
      anti-CCA activity in CL6-xenografed nude mice as determined by significant
      inhibitory activity on tumor growth and lung metastasis, as well as prolongation 
      of survival time.
FAU - Amuamuta, Asmare
AU  - Amuamuta A
AD  - Center of Excellence in Pharmacology and Molecular Biology of Malaria and
      Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat
      University, Phahonyothin Road, Klonglung District, Pathum Thani, 12120, Thailand.
FAU - Plengsuriyakarn, Tullayakorn
AU  - Plengsuriyakarn T
AD  - Center of Excellence in Pharmacology and Molecular Biology of Malaria and
      Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat
      University, Phahonyothin Road, Klonglung District, Pathum Thani, 12120, Thailand.
FAU - Na-Bangchang, Kesara
AU  - Na-Bangchang K
AD  - Center of Excellence in Pharmacology and Molecular Biology of Malaria and
      Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat
      University, Phahonyothin Road, Klonglung District, Pathum Thani, 12120, Thailand.
      [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170412
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Antineoplastic Agents, Phytogenic)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*isolation & purification/pharmacology/toxicity
MH  - Bile Duct Neoplasms/*drug therapy
MH  - Cell Line, Tumor
MH  - Cholangiocarcinoma/*drug therapy
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred ICR
MH  - Mice, Nude
MH  - Rhizome/chemistry
MH  - Xenograft Model Antitumor Assays
MH  - Zingiberaceae/*chemistry
PMC - PMC5389435
OTO - NOTNLM
OT  - Anti-CCA
OT  - CL-6
OT  - Cholangiocarcinoma
OT  - Cytoxicity
OT  - Ethyl-p-methoxycinnamate (EPMC)
OT  - Kaempferia galangal Linn
OT  - Nude mouse xenograft model
EDAT- 2017/04/14 06:00
MHDA- 2017/04/21 06:00
CRDT- 2017/04/14 06:00
PHST- 2016/10/11 [received]
PHST- 2017/03/31 [accepted]
AID - 10.1186/s12906-017-1713-4 [doi]
AID - 10.1186/s12906-017-1713-4 [pii]
PST - epublish
SO  - BMC Complement Altern Med. 2017 Apr 12;17(1):213. doi: 10.1186/s12906-017-1713-4.

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