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Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation against Trichuris muris infection.

Abstract Despite the importance of the adjuvant in the immunization process, very few adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant serine/threonine phosphatase 2A from the nematode Angiostrongylus costaricensis (rPP2A) was used for intranasal immunization in mice previously infected with Trichuris muris The animal intranasal immunization with rPP2A-OVS showed a reduction of 99.01% in the number of the nematode eggs and 97.90% in adult. The immunohistochemical analysis of the intestinal sections showed that in immunized animals with lipopeptide the mucus was significantly higher than in the other experimental groups. Also, these animals presented significantly different chemokine, CCL20 and CCL11, levels. However, although the number and size of Tuft cells did not vary between groups, the intensity of fluorescence per cell was significant in the group immunized with the rPP2A-OVS. The results of the present study suggest that mice immunized with the lipopeptide are capable of activating a combined Th17/Th9 response. This strategy of immunization may be of great applicability not only in immunotherapy and immunoprophylaxis to control diseases caused by nematodes but also in pathologies necessitating action at the level of the Th9 response in the intestinal mucosa.
PMID
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Authors

Mayor MeshTerms
Keywords

Trichuris muris vaccination

chemokines

cytokines

lipid vinyl sulfone

self-assembling lipopeptide rPP2A

Journal Title open biology
Publication Year Start




PMID- 28404797
OWN - NLM
STAT- MEDLINE
DA  - 20170413
DCOM- 20170420
LR  - 20170420
IS  - 2046-2441 (Electronic)
IS  - 2046-2441 (Linking)
VI  - 7
IP  - 4
DP  - 2017 Apr
TI  - Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced
      immunomodulation against Trichuris muris infection.
LID - 170031 [pii]
LID - 10.1098/rsob.170031 [doi]
AB  - Despite the importance of the adjuvant in the immunization process, very few
      adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant
      oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant
      serine/threonine phosphatase 2A from the nematode Angiostrongylus costaricensis
      (rPP2A) was used for intranasal immunization in mice previously infected with
      Trichuris muris The animal intranasal immunization with rPP2A-OVS showed a
      reduction of 99.01% in the number of the nematode eggs and 97.90% in adult. The
      immunohistochemical analysis of the intestinal sections showed that in immunized 
      animals with lipopeptide the mucus was significantly higher than in the other
      experimental groups. Also, these animals presented significantly different
      chemokine, CCL20 and CCL11, levels. However, although the number and size of Tuft
      cells did not vary between groups, the intensity of fluorescence per cell was
      significant in the group immunized with the rPP2A-OVS. The results of the present
      study suggest that mice immunized with the lipopeptide are capable of activating 
      a combined Th17/Th9 response. This strategy of immunization may be of great
      applicability not only in immunotherapy and immunoprophylaxis to control diseases
      caused by nematodes but also in pathologies necessitating action at the level of 
      the Th9 response in the intestinal mucosa.
CI  - (c) 2017 The Authors.
FAU - Gomez-Samblas, M
AU  - Gomez-Samblas M
AD  - Instituto de Biotecnologia, Grupo de Bioquimica y Parasitologia Molecular,
      Departamento de Parasitologia, Universidad de Granada, Campus Universitario
      Fuentenueva, 18071 Granada, Spain.
FAU - Garcia-Rodriguez, J J
AU  - Garcia-Rodriguez JJ
AD  - Departamento de Parasitologia, Facultad de Farmacia, Universidad Complutense,
      Plaza de Ramon y Cajal s/n. Ciudad Universitaria, 28040 Madrid, Spain.
FAU - Trelis, M
AU  - Trelis M
AD  - Area de Parasitologia, Departament de Farmacia i Tecnologia Farmaceutica i
      Parasitologia, Universitat de Valencia, Av. V.A. Estelles, s/n, 46100 Burjassot
      (Valencia), Spain.
AD  - Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics, Health
      Research Institute-La Fe, Universitat de Valencia, Av. Fdo. Abril Martorell, 106,
      46026 Valencia, Spain.
FAU - Bernal, D
AU  - Bernal D
AD  - Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, C/ Dr
      Moliner, 50, 46100 Burjassot (Valencia), Spain.
FAU - Lopez-Jaramillo, F J
AU  - Lopez-Jaramillo FJ
AD  - Departamento de Quimica Organica, Facultad de Ciencias, Instituto de
      Biotecnologia, Universidad de Granada, 18071 Granada, Spain.
FAU - Santoyo-Gonzalez, F
AU  - Santoyo-Gonzalez F
AD  - Departamento de Quimica Organica, Facultad de Ciencias, Instituto de
      Biotecnologia, Universidad de Granada, 18071 Granada, Spain.
FAU - Vilchez, S
AU  - Vilchez S
AD  - Instituto de Biotecnologia, Grupo de Bioquimica y Parasitologia Molecular,
      Departamento de Bioquimica, Universidad de Granada, Campus Universitario
      Fuentenueva, 18071 Granada, Spain.
FAU - Espino, A M
AU  - Espino AM
AD  - Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, 
      University of Puerto Rico, School of Medicine. PO Box 365067, San Juan
      00936-5067, Puerto Rico.
FAU - Bolas-Fernandez, F
AU  - Bolas-Fernandez F
AD  - Departamento de Parasitologia, Facultad de Farmacia, Universidad Complutense,
      Plaza de Ramon y Cajal s/n. Ciudad Universitaria, 28040 Madrid, Spain.
FAU - Osuna, A
AU  - Osuna A
AUID- ORCID: http://orcid.org/0000-0002-3818-1471
AD  - Instituto de Biotecnologia, Grupo de Bioquimica y Parasitologia Molecular,
      Departamento de Parasitologia, Universidad de Granada, Campus Universitario
      Fuentenueva, 18071 Granada, Spain [email protected]
LA  - eng
PT  - Journal Article
PL  - England
TA  - Open Biol
JT  - Open biology
JID - 101580419
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (CCL20 protein, mouse)
RN  - 0 (Ccl11 protein, mouse)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Helminth Proteins)
RN  - 0 (Interleukins)
RN  - 0 (Lipopeptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sulfones)
RN  - 0 (Vaccines, Conjugate)
RN  - 5PFN71LP8M (divinyl sulfone)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage/chemical synthesis
MH  - Administration, Intranasal
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chemokine CCL11/genetics/immunology
MH  - Chemokine CCL20/genetics/immunology
MH  - Female
MH  - Gene Expression
MH  - Helminth Proteins/*administration & dosage/biosynthesis/immunology
MH  - Interleukins/genetics/immunology
MH  - Intestinal Mucosa/drug effects/immunology/parasitology
MH  - Lipopeptides/*administration & dosage/biosynthesis/immunology
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Parasite Egg Count
MH  - Protein Phosphatase 2/*administration & dosage/biosynthesis/immunology
MH  - Recombinant Proteins/administration & dosage/biosynthesis/immunology
MH  - Sequence Alignment
MH  - Sulfones/*administration & dosage/chemistry/immunology
MH  - Th17 Cells/drug effects/immunology/parasitology
MH  - Trichuriasis/immunology/parasitology/*prevention & control
MH  - Trichuris/drug effects/immunology
MH  - Vaccines, Conjugate/*administration & dosage
OTO - NOTNLM
OT  - Trichuris muris vaccination
OT  - chemokines
OT  - cytokines
OT  - lipid vinyl sulfone
OT  - self-assembling lipopeptide rPP2A
EDAT- 2017/04/14 06:00
MHDA- 2017/04/21 06:00
CRDT- 2017/04/14 06:00
PHST- 2017/02/08 [received]
PHST- 2017/03/14 [accepted]
AID - rsob.170031 [pii]
AID - 10.1098/rsob.170031 [doi]
PST - ppublish
SO  - Open Biol. 2017 Apr;7(4). pii: 170031. doi: 10.1098/rsob.170031.

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