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Helicobacter is preserved in yeast vacuoles! Does Koch's postulates confirm it?

Abstract The manuscript titled "Vacuoles of Candida yeast behave as a specialized niche for Helicobacter pylori (H. pylori)" not only has not been prepared in a scientific manner but the methodology used was not adequate, and therefore the conclusion reached was not correct. First of all, "yeast" is a broad terminology covering a great number of genera and species of unicellular micro-organisms. The authors should have defined the organism with its binary scientific name. This measure would allow experiment reproduction by the scientific community. Moreover, the criteria established by Robert Koch to identify a specific microorganism or pathogen was not adopted in the methodology used. Regarding the methodology applied, use of the chicken egg-yolk (IgY) antibody and PCR of the apparently tainted yeast population to prove H. pylori existence in the yeast vacuoles might be main factors for their wrong conclusions. Bacterial tropism toward yeast extract is a known phenomenon, and yeast extract is one of the main ingredients in culture media. Their internalization through phagocytosis or similar pathways does not seem possible or practical because of the thick and cellulosic yeast wall. While the small size of yeast cells does not support their ability in harboring several H. pylori, other observations such as inefficiency of anti-fungal therapy as anti-Helicobacter therapy strongly reject the conclusion reached by the above-mentioned article.
PMID
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Vacuoles of Candida yeast as a specialized niche for Helicobacter pylori.

Authors

Mayor MeshTerms

Helicobacter

Keywords

Acanthamoeba castellanii

Helicobacter pylori

Koch’s postulates

Yeast

Journal Title world journal of gastroenterology
Publication Year Start




PMID- 28405156
OWN - NLM
STAT- MEDLINE
DA  - 20170413
DCOM- 20170414
LR  - 20170416
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 23
IP  - 12
DP  - 2017 Mar 28
TI  - Helicobacter is preserved in yeast vacuoles! Does Koch's postulates confirm it?
PG  - 2266-2268
LID - 10.3748/wjg.v23.i12.2266 [doi]
AB  - The manuscript titled "Vacuoles of Candida yeast behave as a specialized niche
      for Helicobacter pylori (H. pylori)" not only has not been prepared in a
      scientific manner but the methodology used was not adequate, and therefore the
      conclusion reached was not correct. First of all, "yeast" is a broad terminology 
      covering a great number of genera and species of unicellular micro-organisms. The
      authors should have defined the organism with its binary scientific name. This
      measure would allow experiment reproduction by the scientific community.
      Moreover, the criteria established by Robert Koch to identify a specific
      microorganism or pathogen was not adopted in the methodology used. Regarding the 
      methodology applied, use of the chicken egg-yolk (IgY) antibody and PCR of the
      apparently tainted yeast population to prove H. pylori existence in the yeast
      vacuoles might be main factors for their wrong conclusions. Bacterial tropism
      toward yeast extract is a known phenomenon, and yeast extract is one of the main 
      ingredients in culture media. Their internalization through phagocytosis or
      similar pathways does not seem possible or practical because of the thick and
      cellulosic yeast wall. While the small size of yeast cells does not support their
      ability in harboring several H. pylori, other observations such as inefficiency
      of anti-fungal therapy as anti-Helicobacter therapy strongly reject the
      conclusion reached by the above-mentioned article.
FAU - Alipour, Nader
AU  - Alipour N
AD  - Nader Alipour, Department of Medical Microbiology and Biotechnology, DEU
      University and Graduate of Middle East Technical university (METU), Ankara 06800,
      Turkey.
FAU - Gaeini, Nasrin
AU  - Gaeini N
AD  - Nader Alipour, Department of Medical Microbiology and Biotechnology, DEU
      University and Graduate of Middle East Technical university (METU), Ankara 06800,
      Turkey.
LA  - eng
PT  - Journal Article
PT  - Comment
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
SB  - IM
CON - World J Gastroenterol. 2014 May 14;20(18):5263-73. PMID: 24833856
MH  - *Helicobacter
MH  - Helicobacter Infections/*immunology
MH  - Helicobacter pylori/immunology
MH  - Humans
MH  - Saccharomyces cerevisiae
MH  - Vacuoles/microbiology
PMC - PMC5374140
OTO - NOTNLM
OT  - Acanthamoeba castellanii
OT  - Helicobacter pylori
OT  - Koch's postulates
OT  - Yeast
COI - Conflict-of-interest statement: There is no conflict of interest.
EDAT- 2017/04/14 06:00
MHDA- 2017/04/15 06:00
CRDT- 2017/04/14 06:00
PHST- 2016/11/08 [received]
PHST- 2017/01/10 [revised]
PHST- 2017/02/16 [accepted]
AID - 10.3748/wjg.v23.i12.2266 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2017 Mar 28;23(12):2266-2268. doi:
      10.3748/wjg.v23.i12.2266.

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