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Protection induced by virus-like particles containing Toxoplasma gondii microneme protein 8 against highly virulent RH strain of Toxoplasma gondii infection.

Abstract Toxoplasma gondii (T. gondii) microneme protein 8 (MIC8) represents a novel, functional distinct invasion factor. In this study, we generated virus-like particles (VLPs) targeting Toxoplasma gondii MIC8 for the first time, and investigated the protection against highly virulent RH strain of T. gondii in a mouse model. We found that VLP vaccination induced Toxoplasma gondii-specific IgG and IgG1 antibody responses in the sera. Upon challenge infection with RH strain of T. gondii tachyzoites, vaccinated mice showed a significant increase of both IgG antibodies in sera and IgA antibodies in feces compared to those before challenge, and a rapid expansion of both germinal center B cell (B220+, GL7+) and T cell (CD4+, CD8+) populations. Importantly, intranasally immunized mice showed higher neutralizing antibodies and displayed no proinflammatory cytokine IFN-γ in the spleen. Mice were completely protected from a lethal challenge infection with the highly virulent T. gondii (RH) showing no body weight loss (100% survival). Our study shows the effective protection against T. gondii infection provided by VLPs containing microneme protein 8 of T. gondii, thus indicating a potential T. gondii vaccine candidate.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos one
Publication Year Start




PMID- 28406951
OWN - NLM
STAT- MEDLINE
DA  - 20170413
DCOM- 20170421
LR  - 20170421
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Protection induced by virus-like particles containing Toxoplasma gondii microneme
      protein 8 against highly virulent RH strain of Toxoplasma gondii infection.
PG  - e0175644
LID - 10.1371/journal.pone.0175644 [doi]
AB  - Toxoplasma gondii (T. gondii) microneme protein 8 (MIC8) represents a novel,
      functional distinct invasion factor. In this study, we generated virus-like
      particles (VLPs) targeting Toxoplasma gondii MIC8 for the first time, and
      investigated the protection against highly virulent RH strain of T. gondii in a
      mouse model. We found that VLP vaccination induced Toxoplasma gondii-specific IgG
      and IgG1 antibody responses in the sera. Upon challenge infection with RH strain 
      of T. gondii tachyzoites, vaccinated mice showed a significant increase of both
      IgG antibodies in sera and IgA antibodies in feces compared to those before
      challenge, and a rapid expansion of both germinal center B cell (B220+, GL7+) and
      T cell (CD4+, CD8+) populations. Importantly, intranasally immunized mice showed 
      higher neutralizing antibodies and displayed no proinflammatory cytokine
      IFN-gamma in the spleen. Mice were completely protected from a lethal challenge
      infection with the highly virulent T. gondii (RH) showing no body weight loss
      (100% survival). Our study shows the effective protection against T. gondii
      infection provided by VLPs containing microneme protein 8 of T. gondii, thus
      indicating a potential T. gondii vaccine candidate.
FAU - Lee, Su-Hwa
AU  - Lee SH
AD  - Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul,
      Korea.
FAU - Kim, Ah-Ra
AU  - Kim AR
AD  - Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul,
      Korea.
FAU - Lee, Dong-Hun
AU  - Lee DH
AD  - Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul,
      Korea.
FAU - Rubino, Ilaria
AU  - Rubino I
AD  - Department of Chemical and Materials Engineering, University of Alberta,
      Edmonton, AB, Canada.
FAU - Choi, Hyo-Jick
AU  - Choi HJ
AD  - Department of Chemical and Materials Engineering, University of Alberta,
      Edmonton, AB, Canada.
FAU - Quan, Fu-Shi
AU  - Quan FS
AUID- ORCID: http://orcid.org/0000-0003-0419-9339
AD  - Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul,
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20170413
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Protozoan Vaccines)
RN  - 0 (Vaccines, Virus-Like Particle)
RN  - 0 (microneme protein 8, Toxoplasma gondii)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Cell Adhesion Molecules/*administration & dosage
MH  - Female
MH  - Immunoglobulin A/metabolism
MH  - Immunoglobulin G/blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Protozoan Proteins/*administration & dosage
MH  - Protozoan Vaccines/administration & dosage
MH  - Toxoplasma/immunology/*pathogenicity
MH  - Toxoplasmosis, Animal/immunology/*prevention & control
MH  - Vaccines, Virus-Like Particle/*administration & dosage
MH  - Virulence
EDAT- 2017/04/14 06:00
MHDA- 2017/04/22 06:00
CRDT- 2017/04/14 06:00
PHST- 2017/01/20 [received]
PHST- 2017/03/29 [accepted]
AID - 10.1371/journal.pone.0175644 [doi]
AID - PONE-D-17-02691 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 13;12(4):e0175644. doi: 10.1371/journal.pone.0175644.
      eCollection 2017.

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