PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 28406954
OWN - NLM
STAT- MEDLINE
DA  - 20170413
DCOM- 20170420
LR  - 20170420
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Therapeutic efficacy of intra-articular hyaluronan derivative and platelet-rich
      plasma in mice following axial tibial loading.
PG  - e0175682
LID - 10.1371/journal.pone.0175682 [doi]
AB  - OBJECTIVE: To investigate the therapeutic potential of intra-articular
      hyaluronan-derivative HYADD(R) 4-G and/or platelet-rich plasma (PRP) in a mouse
      model of non-invasive joint injury. METHODS: Non-invasive axial tibial loading
      was used to induce joint injury in 10-week-old C57BL/6J mice (n = 86). Mice
      underwent a single loading of either 6 Newton (N) or 9N axial tibial compression.
      HYADD(R) 4-G was injected intra-articularly at 8 mg/mL or 15 mg/mL either before 
      or after loading with or without PRP. Phosphate-buffered-saline was injected as
      control. Knee joints were harvested at 5 or 56 days post-loading and prepared for
      micro-computed tomography scanning and subsequently processed for histology.
      Immunostaining was performed for aggrecan to monitor its distribution, for CD44
      to monitor chondrocyte reactive changes and for COMP (cartilage oligomeric matrix
      protein) as an index for cartilage matrix changes related to loading and
      cartilage injury. TUNEL assay was performed to identify chondrocyte apoptosis.
      RESULTS: Loading initiated cartilage proteoglycan loss and chondrocyte apoptosis 
      within 5 days with slowly progressive post-traumatic osteoarthritis (no cartilage
      degeneration, but increased synovitis and ectopic calcification after 9N loading)
      at 56 days. Mice treated with repeated HYADD(R) 4-G (15 mg/mL) or HYADD(R) 4-G (8
      mg/mL) +/- PRP or PRP alone exhibited no significant improvement in the
      short-term (5 days) and long-term (56 days) consequences of joint loading except 
      for a trend for improved bone changes compared to non-loaded joints. CONCLUSION: 
      While we failed to show an overall effect of intra-articular delivery of
      hyaluronan-derivative and/or PRP in reversing/protecting the pathological events 
      in cartilage and synovium following joint injury, some bone alterations were
      relatively less severe with hyaluronan-derivative at higher concentration or in
      association with PRP.
FAU - Duan, Xin
AU  - Duan X
AD  - Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington
      University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri,
      United States of America.
AD  - Department of Orthopedic Surgery, First Affiliated Hospital of Sun Yet-san
      University, Guangzhou, China.
FAU - Sandell, Linda J
AU  - Sandell LJ
AD  - Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington
      University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri,
      United States of America.
AD  - Department of Cell Biology and Physiology, Washington University School of
      Medicine at Barnes-Jewish Hospital, St. Louis, Missouri, United States of
      America.
AD  - Department of Biomedical Engineering, Washington University in St. Louis, St.
      Louis, Missouri, United States of America.
FAU - Chinzei, Nobuaki
AU  - Chinzei N
AD  - Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington
      University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri,
      United States of America.
FAU - Holguin, Nilsson
AU  - Holguin N
AD  - Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington
      University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri,
      United States of America.
FAU - Silva, Matthew J
AU  - Silva MJ
AD  - Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington
      University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri,
      United States of America.
AD  - Department of Biomedical Engineering, Washington University in St. Louis, St.
      Louis, Missouri, United States of America.
FAU - Schiavinato, Antonella
AU  - Schiavinato A
AD  - Fidia Farmaceutici S.p.A., Abano Terme, Padova, Italy.
FAU - Rai, Muhammad Farooq
AU  - Rai MF
AUID- ORCID: http://orcid.org/0000-0003-4826-4331
AD  - Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington
      University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri,
      United States of America.
AD  - Department of Cell Biology and Physiology, Washington University School of
      Medicine at Barnes-Jewish Hospital, St. Louis, Missouri, United States of
      America.
LA  - eng
PT  - Journal Article
DEP - 20170413
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Aggrecans)
RN  - 0 (Antigens, CD44)
RN  - 0 (Cartilage Oligomeric Matrix Protein)
RN  - 0 (Cd44 protein, mouse)
RN  - 0 (HYADD 4)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Aggrecans/metabolism
MH  - Animals
MH  - Antigens, CD44/metabolism
MH  - Apoptosis/drug effects
MH  - Cartilage Diseases/diagnostic imaging/*drug therapy/etiology/metabolism
MH  - Cartilage Oligomeric Matrix Protein/metabolism
MH  - Chondrocytes/cytology/drug effects
MH  - Disease Models, Animal
MH  - Hyaluronic Acid/*administration & dosage/pharmacology
MH  - Injections, Intra-Articular
MH  - Knee Joint/diagnostic imaging/*drug effects
MH  - Mice
MH  - Osteoarthritis, Knee/diagnostic imaging/*drug therapy/etiology/metabolism
MH  - Platelet-Rich Plasma/*physiology
MH  - Tomography, X-Ray Computed
MH  - Treatment Failure
EDAT- 2017/04/14 06:00
MHDA- 2017/04/21 06:00
CRDT- 2017/04/14 06:00
PHST- 2016/12/07 [received]
PHST- 2017/03/29 [accepted]
AID - 10.1371/journal.pone.0175682 [doi]
AID - PONE-D-16-48449 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 13;12(4):e0175682. doi: 10.1371/journal.pone.0175682.
      eCollection 2017.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>