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Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals.

Abstract Schistosomiasis japonica is a common zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and, for this reason, diagnostics with a higher sensitivity have become increasingly necessary. It was reported that polymerase chain reaction (PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity. The present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic animals.
PMID
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Authors

Mayor MeshTerms
Keywords

Diagnosis

Domestic animals

Nested-PCR

Schistosomiasis

Journal Title infectious diseases of poverty
Publication Year Start




PMID- 28407808
OWN - NLM
STAT- In-Process
DA  - 20170414
LR  - 20170416
IS  - 2049-9957 (Electronic)
IS  - 2049-9957 (Linking)
VI  - 6
IP  - 1
DP  - 2017 Apr 13
TI  - Nested-PCR assay for detection of Schistosoma japonicum infection in domestic
      animals.
PG  - 86
LID - 10.1186/s40249-017-0298-y [doi]
AB  - BACKGROUND: Schistosomiasis japonica is a common zoonosis. Domestic animals are
      the primary source of infection and play an important role in disease
      transmission. The prevalence and infectivity of this disease in domestic animals 
      in China have significantly decreased and, for this reason, diagnostics with a
      higher sensitivity have become increasingly necessary. It was reported that
      polymerase chain reaction (PCR)-based methods could be used to detect schistosome
      infection in humans and animals and presented a high sensitivity and specificity.
      The present study aimed to develop a PCR-based method for detection of
      Schistosoma japonicum infection in domestic animals. METHODS: A specific
      nested-PCR assay was developed to detect S. japonicum infection in domestic
      animals via amplification of a 231-bp DNA fragment of retrotransposon SjR2. The
      developed assay was first used in sera and dry blood filter paper (DBFP) from
      goats and buffaloes at different time points of infection. Then, 78 DBFPs from 39
      artificially-infected bovines at 14 and 28 days post-infection and 42 DBFPs from 
      schistosome-negative bovines from the city of Huangshan in the Anhui province
      were used to evaluate the diagnostic validity. Furthermore, this assay was used
      to detect S. japonicum infection in domestic animals in Dongzhi and Wangjiang
      counties. RESULTS: The expected PCR product was detected in eggs and adult worms 
      of S. japonicum and blood samples from S. japonicum-infected goats and water
      buffaloes, but not from Fasciola and Haemonchus contortus worms. The nested-PCR
      assay could detect the target S. japonicum DNA in DBFPs from goats and buffaloes 
      after day 3 post-infection. The sensitivity in buffaloes at 14 and 28 days
      post-infection was 92.30% (36/39) and 100% (39/39), respectively. The specificity
      was 97.60% (41/42). The positivity rates in Dongzhi and Wangjiang counties were
      6.00% and 8.00% in bovines and 22.00% and 16.67% in goats, respectively. The
      positivity rates in goats in both counties were higher than those in bovines with
      a significant difference in Dongzhi County but not in Wangjiang County (P < 0.05 
      and P = 0.23, respectively). CONCLUSIONS: Our results suggest that the developed 
      nested-PCR assay may be used for the diagnosis of S. japonicum infection in
      domestic animals, and the control of S. japonicum infection in goats should be
      paid more attention.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - He, Chuan-Chuan
AU  - He CC
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Liu, Jin-Ming
AU  - Liu JM
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China. [email protected]
FAU - Li, Hao
AU  - Li H
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Lu, Ke
AU  - Lu K
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Fu, Zhi-Qiang
AU  - Fu ZQ
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Zhu, Chuan-Gang
AU  - Zhu CG
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Liu, Yi-Ping
AU  - Liu YP
AD  - Anhui Center for Animal Disease Control and Prevention, Hefei, People's Republic 
      of China.
FAU - Tong, Lai-Bao
AU  - Tong LB
AD  - Wangjiang county Center for Animal Husbandry and Veterinary Bureau, Anqing,
      People's Republic of China.
FAU - Zhou, De-Bao
AU  - Zhou DB
AD  - Dongzhi county Center for Animal Husbandry and Veterinary Bureau, Chizhou,
      People's Republic of China.
FAU - Zha, Li
AU  - Zha L
AD  - Huangshan Center for Animal Disease Control and Prevention, Huangshan, 341000,
      People's Republic of China.
FAU - Hong, Yang
AU  - Hong Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Jin, Ya-Mei
AU  - Jin YM
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
FAU - Lin, Jiao-Jiao
AU  - Lin JJ
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
      Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai, 200241,
      People's Republic of China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal
      Infectious and Zoonosea, Yangzhou, Jiangsu province, 225009, People's Republic of
      China.
LA  - eng
PT  - Journal Article
DEP - 20170413
PL  - England
TA  - Infect Dis Poverty
JT  - Infectious diseases of poverty
JID - 101606645
PMC - PMC5390378
OTO - NOTNLM
OT  - Diagnosis
OT  - Domestic animals
OT  - Nested-PCR
OT  - Schistosomiasis
EDAT- 2017/04/15 06:00
MHDA- 2017/04/15 06:00
CRDT- 2017/04/15 06:00
PHST- 2017/01/12 [received]
PHST- 2017/04/03 [accepted]
AID - 10.1186/s40249-017-0298-y [doi]
AID - 10.1186/s40249-017-0298-y [pii]
PST - epublish
SO  - Infect Dis Poverty. 2017 Apr 13;6(1):86. doi: 10.1186/s40249-017-0298-y.

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