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Interactions between the breast cancer-associated MUC1 mucins and C-type lectin characterized by optical tweezers.

Abstract Carbohydrate-protein interactions govern many crucial processes in biological systems including cell recognition events. We have used the sensitive force probe optical tweezers to quantify the interactions occurring between MGL lectins and MUC1 carrying the cancer-associated glycan antigens mucins Tn and STn. Unbinding forces of 7.6 pN and 7.1 pN were determined for the MUC1(Tn)-MGL and MUC1(STn)-MGL interactions, at a force loading rate of ~40 pN/s. The interaction strength increased with increasing force loading rate, to 27 and 37 pN at a force loading rate of ~ 310 pN/s. No interactions were detected between MGL and MUC1(ST), a glycoform of MUC1 also expressed by breast carcinoma cells. Interestingly, this glycan (ST) can be found on proteins expressed by normal cells, although in this case not on MUC1. Additionally, GalNAc decorated polyethylene glycol displayed similar rupture forces as observed for MUC1(Tn) and MUC1(STn) when forced to unbind from MGL, indicating that GalNAc is an essential group in these interactions. Since the STn glycan decoration is more frequently found on the surface of carcinomas than the Tn glycan, the binding of MUC1 carrying STn to MGL may be more physiologically relevant and may be in part responsible for some of the characteristics of STn expressing tumours.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos one
Publication Year Start




PMID- 28414807
OWN - NLM
STAT- MEDLINE
DA  - 20170417
DCOM- 20170425
LR  - 20170425
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Interactions between the breast cancer-associated MUC1 mucins and C-type lectin
      characterized by optical tweezers.
PG  - e0175323
LID - 10.1371/journal.pone.0175323 [doi]
AB  - Carbohydrate-protein interactions govern many crucial processes in biological
      systems including cell recognition events. We have used the sensitive force probe
      optical tweezers to quantify the interactions occurring between MGL lectins and
      MUC1 carrying the cancer-associated glycan antigens mucins Tn and STn. Unbinding 
      forces of 7.6 pN and 7.1 pN were determined for the MUC1(Tn)-MGL and
      MUC1(STn)-MGL interactions, at a force loading rate of ~40 pN/s. The interaction 
      strength increased with increasing force loading rate, to 27 and 37 pN at a force
      loading rate of ~ 310 pN/s. No interactions were detected between MGL and
      MUC1(ST), a glycoform of MUC1 also expressed by breast carcinoma cells.
      Interestingly, this glycan (ST) can be found on proteins expressed by normal
      cells, although in this case not on MUC1. Additionally, GalNAc decorated
      polyethylene glycol displayed similar rupture forces as observed for MUC1(Tn) and
      MUC1(STn) when forced to unbind from MGL, indicating that GalNAc is an essential 
      group in these interactions. Since the STn glycan decoration is more frequently
      found on the surface of carcinomas than the Tn glycan, the binding of MUC1
      carrying STn to MGL may be more physiologically relevant and may be in part
      responsible for some of the characteristics of STn expressing tumours.
FAU - Hadjialirezaei, Soosan
AU  - Hadjialirezaei S
AD  - Biophysics and Medical Technology, Department of Physics, NTNU Norwegian
      University of Science and Technology, Trondheim, Norway.
FAU - Picco, Gianfranco
AU  - Picco G
AD  - Breast Cancer Biology, King's College London, Guy's Hospital, London, United
      Kingdom.
FAU - Beatson, Richard
AU  - Beatson R
AD  - Breast Cancer Biology, King's College London, Guy's Hospital, London, United
      Kingdom.
FAU - Burchell, Joy
AU  - Burchell J
AD  - Breast Cancer Biology, King's College London, Guy's Hospital, London, United
      Kingdom.
FAU - Stokke, Bjorn Torger
AU  - Stokke BT
AD  - Biophysics and Medical Technology, Department of Physics, NTNU Norwegian
      University of Science and Technology, Trondheim, Norway.
FAU - Sletmoen, Marit
AU  - Sletmoen M
AD  - Department of Biotechnology, NTNU Norwegian University of Science and Technology,
      Trondheim, Norway.
LA  - eng
PT  - Journal Article
DEP - 20170417
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Tumor-Associated, Carbohydrate)
RN  - 0 (Carbohydrates)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MUC1 protein, human)
RN  - 0 (Mucin-1)
RN  - 0 (Polysaccharides)
RN  - 0 (Tn antigen)
SB  - IM
MH  - Animals
MH  - Antigens, Tumor-Associated, Carbohydrate/metabolism
MH  - Breast Neoplasms/*metabolism
MH  - CHO Cells
MH  - Carbohydrates
MH  - Cell Line
MH  - Cricetulus
MH  - Female
MH  - Humans
MH  - Lectins, C-Type/*metabolism
MH  - Mucin-1/*metabolism
MH  - Optical Tweezers
MH  - Polysaccharides/metabolism
EDAT- 2017/04/18 06:00
MHDA- 2017/04/26 06:00
CRDT- 2017/04/18 06:00
PHST- 2016/10/04 [received]
PHST- 2017/03/23 [accepted]
AID - 10.1371/journal.pone.0175323 [doi]
AID - PONE-D-16-39538 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 17;12(4):e0175323. doi: 10.1371/journal.pone.0175323.
      eCollection 2017.

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