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Detection of CTX-M-15 beta-lactamases in Enterobacteriaceae causing hospital- and community-acquired urinary tract infections as early as 2004, in Dar es Salaam, Tanzania.

Abstract The spread of Extended Spectrum β-lactamases (ESBLs) among Enterobacteriaceae and other Gram-Negative pathogens in the community and hospitals represents a major challenge to combat infections. We conducted a study to assess the prevalence and genetic makeup of ESBL-type resistance in bacterial isolates causing community- and hospital-acquired urinary tract infections.
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Authors

Mayor MeshTerms
Keywords

ESBL

Tanzania

Urinary tract infections

Journal Title bmc infectious diseases
Publication Year Start




PMID- 28415986
OWN - NLM
STAT- MEDLINE
DA  - 20170418
DCOM- 20170517
LR  - 20170517
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Apr 17
TI  - Detection of CTX-M-15 beta-lactamases in Enterobacteriaceae causing hospital- and
      community-acquired urinary tract infections as early as 2004, in Dar es Salaam,
      Tanzania.
PG  - 282
LID - 10.1186/s12879-017-2395-8 [doi]
AB  - BACKGROUND: The spread of Extended Spectrum beta-lactamases (ESBLs) among
      Enterobacteriaceae and other Gram-Negative pathogens in the community and
      hospitals represents a major challenge to combat infections. We conducted a study
      to assess the prevalence and genetic makeup of ESBL-type resistance in bacterial 
      isolates causing community- and hospital-acquired urinary tract infections.
      METHODS: A total of 172 isolates of Enterobacteriaceae were collected in Dar es
      Salaam, Tanzania, from patients who met criteria of community and
      hospital-acquired urinary tract infections. We used E-test ESBL strips to test
      for ESBL-phenotype and PCR and sequencing for detection of ESBL genes. RESULTS:
      Overall 23.8% (41/172) of all isolates were ESBL-producers. ESBL-producers were
      more frequently isolated from hospital-acquired infections (32%, 27/84 than from 
      community-acquired infections (16%, 14/88, p < 0.05). ESBL-producers showed high 
      rate of resistance to ciprofloxacin (85.5%), doxycycline (90.2%), gentamicin
      (80.5%), nalidixic acid (84.5%), and trimethoprim-sulfamethoxazole (85.4%).
      Furthermore, 95% of ESBL-producers were multi-drug resistant compared to 69% of
      non-ESBL-producers (p < 0.05). The distribution of ESBL genes were as follows:
      29/32 (90.6%) bla CTX-M-15, two bla SHV-12, and one had both bla CTX-M-15 and bla
      SHV-12. Of 29 isolates carrying bla CTX-M-15, 69% (20/29) and 31% (9/29) were
      hospital and community, respectively. Bla SHV-12 genotypes were only detected in 
      hospital-acquired infections. CONCLUSION: bla CTX-M-15 is a predominant gene
      conferring ESBL-production in Enterobacteriaceae causing both hospital- and
      community-acquired infections in Tanzania.
FAU - Manyahi, Joel
AU  - Manyahi J
AD  - Department of Clinical Science, University of Bergen, Bergen, Norway.
      [email protected]
AD  - Department of Microbiology and Immunology, Muhimbili University of Health and
      Allied Sciences, Dar es Salaam, Tanzania. [email protected]
FAU - Moyo, Sabrina J
AU  - Moyo SJ
AD  - Department of Clinical Science, University of Bergen, Bergen, Norway.
AD  - Department of Microbiology and Immunology, Muhimbili University of Health and
      Allied Sciences, Dar es Salaam, Tanzania.
FAU - Tellevik, Marit Gjerde
AU  - Tellevik MG
AD  - National Centre for Tropical Infectious Diseases, Department of Medicine,
      Haukeland University Hospital, Bergen, Norway.
FAU - Ndugulile, Faustine
AU  - Ndugulile F
AD  - Department of Microbiology and Immunology, Muhimbili University of Health and
      Allied Sciences, Dar es Salaam, Tanzania.
FAU - Urassa, Willy
AU  - Urassa W
AD  - Department of Microbiology and Immunology, Muhimbili University of Health and
      Allied Sciences, Dar es Salaam, Tanzania.
FAU - Blomberg, Bjorn
AU  - Blomberg B
AD  - Department of Clinical Science, University of Bergen, Bergen, Norway.
AD  - National Centre for Tropical Infectious Diseases, Department of Medicine,
      Haukeland University Hospital, Bergen, Norway.
FAU - Langeland, Nina
AU  - Langeland N
AD  - Department of Clinical Science, University of Bergen, Bergen, Norway.
AD  - National Centre for Tropical Infectious Diseases, Department of Medicine,
      Haukeland University Hospital, Bergen, Norway.
LA  - eng
PT  - Journal Article
DEP - 20170417
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
RN  - 8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)
RN  - EC 3.5.2.- (beta-lactamase CTX-M-15)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Bacterial Typing Techniques/*methods
MH  - Community-Acquired Infections/drug therapy/*epidemiology/*microbiology
MH  - Cross Infection/epidemiology/microbiology
MH  - Drug Resistance, Bacterial/genetics
MH  - Drug Resistance, Multiple/genetics
MH  - Enterobacteriaceae/*genetics/isolation & purification
MH  - Enterobacteriaceae Infections/drug therapy/*epidemiology/microbiology
MH  - Female
MH  - Hospitals
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Polymerase Chain Reaction
MH  - Tanzania/epidemiology
MH  - Trimethoprim, Sulfamethoxazole Drug Combination/therapeutic use
MH  - Urinary Tract Infections/drug therapy/*epidemiology/*microbiology
MH  - beta-Lactamases/analysis/*genetics
PMC - PMC5392921
OTO - NOTNLM
OT  - ESBL
OT  - Tanzania
OT  - Urinary tract infections
EDAT- 2017/04/19 06:00
MHDA- 2017/05/18 06:00
CRDT- 2017/04/19 06:00
PHST- 2017/02/18 [received]
PHST- 2017/04/11 [accepted]
AID - 10.1186/s12879-017-2395-8 [doi]
AID - 10.1186/s12879-017-2395-8 [pii]
PST - epublish
SO  - BMC Infect Dis. 2017 Apr 17;17(1):282. doi: 10.1186/s12879-017-2395-8.

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