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Development of mismatch amplification mutation assays for the differentiation of MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains.

Abstract Mycoplasma synoviae is an economically significant pathogen in the poultry industry, inducing respiratory disease and infectious synovitis in chickens and turkeys, and eggshell apex abnormality in chickens. Eradication, medication and vaccination are the options for controlling M. synoviae infection. Currently there are two commercial, live, attenuated vaccines available against M. synoviae: the temperature sensitive MS-H vaccine strain and the NAD independent MS1 vaccine strain. Differentiation of vaccine strains from field isolates is essential during vaccination and eradication programs. The present study provides melt-curve and agarose gel based mismatch amplification mutation assays (MAMA) to discriminate the MS1 vaccine strain from the MS-H vaccine strain and wild-type M. synoviae isolates. The assays are based on the A/C single nucleotide polymorphism at nt11 of a HIT family protein coding gene. The melt- and agarose-MAMAs reliably distinguish the MS1 vaccine strain genotype from the MS-H vaccine strain and wild-type M. synoviae isolate genotype from 102 template number/DNA sample. No cross-reactions with other avian Mycoplasma species were observed. The assays can be performed directly on clinical samples and they can be run simultaneously with the previously described MAMAs designed for the discrimination of the MS-H vaccine strain. The developed assays are applicable in laboratories with limited facilities and promote the rapid, simple and cost effective differentiation of the MS1 vaccine strain.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos one
Publication Year Start




PMID- 28419134
OWN - NLM
STAT- In-Process
DA  - 20170418
LR  - 20170418
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Development of mismatch amplification mutation assays for the differentiation of 
      MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains.
PG  - e0175969
LID - 10.1371/journal.pone.0175969 [doi]
AB  - Mycoplasma synoviae is an economically significant pathogen in the poultry
      industry, inducing respiratory disease and infectious synovitis in chickens and
      turkeys, and eggshell apex abnormality in chickens. Eradication, medication and
      vaccination are the options for controlling M. synoviae infection. Currently
      there are two commercial, live, attenuated vaccines available against M.
      synoviae: the temperature sensitive MS-H vaccine strain and the NAD independent
      MS1 vaccine strain. Differentiation of vaccine strains from field isolates is
      essential during vaccination and eradication programs. The present study provides
      melt-curve and agarose gel based mismatch amplification mutation assays (MAMA) to
      discriminate the MS1 vaccine strain from the MS-H vaccine strain and wild-type M.
      synoviae isolates. The assays are based on the A/C single nucleotide polymorphism
      at nt11 of a HIT family protein coding gene. The melt- and agarose-MAMAs reliably
      distinguish the MS1 vaccine strain genotype from the MS-H vaccine strain and
      wild-type M. synoviae isolate genotype from 102 template number/DNA sample. No
      cross-reactions with other avian Mycoplasma species were observed. The assays can
      be performed directly on clinical samples and they can be run simultaneously with
      the previously described MAMAs designed for the discrimination of the MS-H
      vaccine strain. The developed assays are applicable in laboratories with limited 
      facilities and promote the rapid, simple and cost effective differentiation of
      the MS1 vaccine strain.
FAU - Kreizinger, Zsuzsa
AU  - Kreizinger Z
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research,
      Hungarian Academy of Sciences, Budapest, Hungary.
FAU - Sulyok, Kinga Maria
AU  - Sulyok KM
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research,
      Hungarian Academy of Sciences, Budapest, Hungary.
FAU - Grozner, Denes
AU  - Grozner D
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research,
      Hungarian Academy of Sciences, Budapest, Hungary.
FAU - Beko, Katinka
AU  - Beko K
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research,
      Hungarian Academy of Sciences, Budapest, Hungary.
FAU - Dan, Adam
AU  - Dan A
AD  - Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest,
      Hungary.
FAU - Szabo, Zoltan
AU  - Szabo Z
AD  - Intervet Hungaria Kft., part of MSD Animal Health, Budapest, Hungary.
FAU - Gyuranecz, Miklos
AU  - Gyuranecz M
AUID- ORCID: http://orcid.org/0000-0002-5698-5713
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research,
      Hungarian Academy of Sciences, Budapest, Hungary.
LA  - eng
PT  - Journal Article
DEP - 20170418
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
EDAT- 2017/04/19 06:00
MHDA- 2017/04/19 06:00
CRDT- 2017/04/19 06:00
PHST- 2017/01/03 [received]
PHST- 2017/04/03 [accepted]
AID - 10.1371/journal.pone.0175969 [doi]
AID - PONE-D-17-00276 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 18;12(4):e0175969. doi: 10.1371/journal.pone.0175969.
      eCollection 2017.

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