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Molecular characteristic of mcr-1 producing Escherichia coli in a Chinese university hospital.

Abstract Colistin has been considered as a last-line treatment option in severe infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, the emergence of the mobile colistin resistance gene (mcr-1) has challenged this viewpoint. The aim of this study is to explore the prevalence of mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and investigate their molecular characteristics.
PMID
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Authors

Mayor MeshTerms

Drug Resistance, Bacterial

Keywords

Colistin

E. coli

Multidrug-resistant

mcr-1

Journal Title annals of clinical microbiology and antimicrobials
Publication Year Start




PMID- 28420384
OWN - NLM
STAT- MEDLINE
DA  - 20170419
DCOM- 20170424
LR  - 20170424
IS  - 1476-0711 (Electronic)
IS  - 1476-0711 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Apr 19
TI  - Molecular characteristic of mcr-1 producing Escherichia coli in a Chinese
      university hospital.
PG  - 32
LID - 10.1186/s12941-017-0207-z [doi]
AB  - BACKGROUND: Colistin has been considered as a last-line treatment option in
      severe infections caused by multidrug-resistant (MDR) gram-negative pathogens.
      However, the emergence of the mobile colistin resistance gene (mcr-1) has
      challenged this viewpoint. The aim of this study is to explore the prevalence of 
      mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and
      investigate their molecular characteristics. METHODS: A total of 700 E. coli
      isolates were used to screen mcr-1 by PCR and sequencing in a Chinese university 
      hospital from August 2014 to August 2015. Susceptibility test of mcr-1-producing 
      isolates was determined by Vitek -2 Compact system. 26 virulence factors (VFs),
      phylogenetic groups, Multi-locus sequence typing (MLST), and DNA Fingerprinting
      (ERIC-PCR) of strains were investigated by PCR. RESULTS: Four (0.6%) mcr-1
      producing E. coli isolates were found in this study. The results of antibiotic
      susceptibility test showed that all four isolates were resistant to colistin,
      ciprofloxacin, levofloxacin, cefazolin, and trimethoprim/sulfamethoxazole, and
      were susceptible to amikacin, ertapenem and imipenem. In addition, all 4 isolates
      exhibited high-level resistance to aztreonam, cefotaxime and gentamicin. The
      numbers of VFs contained in mcr-1 positive isolates were no more than 4 in our
      study. MLST result demonstrated that these isolates were assigned to two sequence
      types: ST156 and ST167. The result of phylogenetic analysis showed that four
      mcr-1-positive isolates belong to two phylogenetic groups: A and B1 group.
      ERIC-PCR showed that four mcr-1 positive strains were categorized into three
      different genotypes. CONCLUSIONS: Our study demonstrated a low prevalence of
      mcr-1 in E. coli clinical isolates in a Chinese teaching hospital, and we have
      gained insights into the molecular characteristics of these mcr-1-positive
      strains. Increasing the surveillance of these infections, as well as taking
      effective infection control measures are urgently needed to take to control the
      transmission of mcr-1 gene.
FAU - He, Qing-Wen
AU  - He QW
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29# 
      Xinquan Road, Fuzhou, 350001, Fujian, China.
AD  - The Union Clinical Medical College of Fujian Medical University, Fuzhou, 350004, 
      Fujian, China.
FAU - Xu, Xiao-Hong
AU  - Xu XH
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29# 
      Xinquan Road, Fuzhou, 350001, Fujian, China.
FAU - Lan, Fang-Jun
AU  - Lan FJ
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29# 
      Xinquan Road, Fuzhou, 350001, Fujian, China.
AD  - The Union Clinical Medical College of Fujian Medical University, Fuzhou, 350004, 
      Fujian, China.
FAU - Zhao, Zhi-Chang
AU  - Zhao ZC
AD  - Department of Pharmacy, Fujian Medical University, Union Hospital, Fuzhou,
      350001, Fujian, China.
FAU - Wu, Zhi-Yun
AU  - Wu ZY
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29# 
      Xinquan Road, Fuzhou, 350001, Fujian, China.
AD  - The Union Clinical Medical College of Fujian Medical University, Fuzhou, 350004, 
      Fujian, China.
FAU - Cao, Ying-Ping
AU  - Cao YP
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29# 
      Xinquan Road, Fuzhou, 350001, Fujian, China.
FAU - Li, Bin
AU  - Li B
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29# 
      Xinquan Road, Fuzhou, 350001, Fujian, China. [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170419
PL  - England
TA  - Ann Clin Microbiol Antimicrob
JT  - Annals of clinical microbiology and antimicrobials
JID - 101152152
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (MCR-1 protein, E coli)
SB  - IM
MH  - Adult
MH  - Aged, 80 and over
MH  - Anti-Bacterial Agents/pharmacology
MH  - China/epidemiology
MH  - *Drug Resistance, Bacterial
MH  - Escherichia coli/classification/*genetics/*isolation & purification
MH  - Escherichia coli Infections/epidemiology/*microbiology
MH  - Escherichia coli Proteins/*analysis/genetics
MH  - Female
MH  - Hospitals, University
MH  - Humans
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Molecular Typing
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Sequence Analysis, DNA
PMC - PMC5395964
OTO - NOTNLM
OT  - Colistin
OT  - E. coli
OT  - Multidrug-resistant
OT  - mcr-1
EDAT- 2017/04/20 06:00
MHDA- 2017/04/25 06:00
CRDT- 2017/04/20 06:00
PHST- 2016/10/16 [received]
PHST- 2017/04/08 [accepted]
AID - 10.1186/s12941-017-0207-z [doi]
AID - 10.1186/s12941-017-0207-z [pii]
PST - epublish
SO  - Ann Clin Microbiol Antimicrob. 2017 Apr 19;16(1):32. doi:
      10.1186/s12941-017-0207-z.

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