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Role of Demyelination Efficiency within Acellular Nerve Scaffolds during Nerve Regeneration across Peripheral Defects.

Abstract Hudson's optimized chemical processing method is the most commonly used chemical method to prepare acellular nerve scaffolds for the reconstruction of large peripheral nerve defects. However, residual myelin attached to the basal laminar tube has been observed in acellular nerve scaffolds prepared using Hudson's method. Here, we describe a novel method of producing acellular nerve scaffolds that eliminates residual myelin more effectively than Hudson's method through the use of various detergent combinations of sulfobetaine-10, sulfobetaine-16, Triton X-200, sodium deoxycholate, and peracetic acid. In addition, the efficacy of this new scaffold in repairing a 1.5 cm defect in the sciatic nerve of rats was examined. The modified method produced a higher degree of demyelination than Hudson's method, resulting in a minor host immune response in vivo and providing an improved environment for nerve regeneration and, consequently, better functional recovery. A morphological study showed that the number of regenerated axons in the modified group and Hudson group did not differ. However, the autograft and modified groups were more similar in myelin sheath regeneration than the autograft and Hudson groups. These results suggest that the modified method for producing a demyelinated acellular scaffold may aid functional recovery in general after nerve defects.
PMID
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Authors

Mayor MeshTerms

Nerve Regeneration

Keywords
Journal Title biomed research international
Publication Year Start




PMID- 28421195
OWN - NLM
STAT- MEDLINE
DA  - 20170419
DCOM- 20170501
LR  - 20170501
IS  - 2314-6141 (Electronic)
VI  - 2017
DP  - 2017
TI  - Role of Demyelination Efficiency within Acellular Nerve Scaffolds during Nerve
      Regeneration across Peripheral Defects.
PG  - 4606387
LID - 10.1155/2017/4606387 [doi]
AB  - Hudson's optimized chemical processing method is the most commonly used chemical 
      method to prepare acellular nerve scaffolds for the reconstruction of large
      peripheral nerve defects. However, residual myelin attached to the basal laminar 
      tube has been observed in acellular nerve scaffolds prepared using Hudson's
      method. Here, we describe a novel method of producing acellular nerve scaffolds
      that eliminates residual myelin more effectively than Hudson's method through the
      use of various detergent combinations of sulfobetaine-10, sulfobetaine-16, Triton
      X-200, sodium deoxycholate, and peracetic acid. In addition, the efficacy of this
      new scaffold in repairing a 1.5 cm defect in the sciatic nerve of rats was
      examined. The modified method produced a higher degree of demyelination than
      Hudson's method, resulting in a minor host immune response in vivo and providing 
      an improved environment for nerve regeneration and, consequently, better
      functional recovery. A morphological study showed that the number of regenerated 
      axons in the modified group and Hudson group did not differ. However, the
      autograft and modified groups were more similar in myelin sheath regeneration
      than the autograft and Hudson groups. These results suggest that the modified
      method for producing a demyelinated acellular scaffold may aid functional
      recovery in general after nerve defects.
FAU - Cai, Meiqin
AU  - Cai M
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-sen
      University, Guangzhou, Guangdong 510630, China.
FAU - Huang, Tengchao
AU  - Huang T
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-sen
      University, Guangzhou, Guangdong 510630, China.
FAU - Hou, Bo
AU  - Hou B
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-sen
      University, Guangzhou, Guangdong 510630, China.
FAU - Guo, Ying
AU  - Guo Y
AUID- ORCID: 0000-0001-8797-9052
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-sen
      University, Guangzhou, Guangdong 510630, China.
LA  - eng
PT  - Journal Article
DEP - 20170321
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Detergents)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/pathology
MH  - Detergents/chemistry
MH  - Male
MH  - *Nerve Regeneration
MH  - Peripheral Nerve Injuries/metabolism/pathology/*therapy
MH  - Peripheral Nerves/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Scaffolds/*chemistry
PMC - PMC5379125
COI - All authors declare that they have no conflict of interests.
EDAT- 2017/04/20 06:00
MHDA- 2017/05/02 06:00
CRDT- 2017/04/20 06:00
PHST- 2016/11/29 [received]
PHST- 2017/01/20 [revised]
PHST- 2017/01/29 [accepted]
AID - 10.1155/2017/4606387 [doi]
PST - ppublish
SO  - Biomed Res Int. 2017;2017:4606387. doi: 10.1155/2017/4606387. Epub 2017 Mar 21.

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