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Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise.

Abstract Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model. Methods. A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks. Behavioral experiments were conducted in order to evaluate the model. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS) in combination with multivariate statistical analysis before and after exercise. Results. A total of 20 metabolites were detected in CFS rats before and after exercise. Three metabolic pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid hormone biosynthesis) were significantly impacted before and after exercise, while sphingolipid metabolism alone exhibited significant alterations after exercise only. Conclusion. In addition to metabolic disturbances involving some energy substances, alterations in steroid hormone biosynthesis and sphingolipid metabolism were detected in CFS rats. Sphingosine and 21-hydroxypregnenolone may be key biomarkers of CFS, potentially offering evidence in support of immune dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in patients with CFS.
PMID
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Authors

Mayor MeshTerms

Physical Conditioning, Animal

Keywords
Journal Title biomed research international
Publication Year Start




PMID- 28421200
OWN - NLM
STAT- MEDLINE
DA  - 20170419
DCOM- 20170501
LR  - 20170501
IS  - 2314-6141 (Electronic)
VI  - 2017
DP  - 2017
TI  - Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before 
      and after Exercise.
PG  - 8182020
LID - 10.1155/2017/8182020 [doi]
AB  - Purpose. The aim of the present study was to elucidate the metabolic mechanisms
      associated with chronic fatigue syndrome (CFS) via an analysis of urine
      metabolites prior to and following exercise in a rat model. Methods. A rat model 
      of CFS was established using restraint-stress, forced exercise, and crowded and
      noisy environments over a period of 4 weeks. Behavioral experiments were
      conducted in order to evaluate the model. Urine metabolites were analyzed via gas
      chromatography-mass spectrometry (GC-MS) in combination with multivariate
      statistical analysis before and after exercise. Results. A total of 20
      metabolites were detected in CFS rats before and after exercise. Three metabolic 
      pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid
      hormone biosynthesis) were significantly impacted before and after exercise,
      while sphingolipid metabolism alone exhibited significant alterations after
      exercise only. Conclusion. In addition to metabolic disturbances involving some
      energy substances, alterations in steroid hormone biosynthesis and sphingolipid
      metabolism were detected in CFS rats. Sphingosine and 21-hydroxypregnenolone may 
      be key biomarkers of CFS, potentially offering evidence in support of immune
      dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in
      patients with CFS.
FAU - Shao, Changzhuan
AU  - Shao C
AD  - College of Arts and Sciences, Shanghai Maritime University, Shanghai 201306,
      China.
FAU - Ren, Yiming
AU  - Ren Y
AD  - Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119,
      China.
FAU - Wang, Zinan
AU  - Wang Z
AD  - Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119,
      China.
FAU - Kang, Chenzhe
AU  - Kang C
AD  - School of Sports, Hebei Normal University, Shijiazhuang 050090, China.
FAU - Jiang, Hongke
AU  - Jiang H
AUID- ORCID: 0000-0003-1650-4919
AD  - College of Arts and Sciences, Shanghai Maritime University, Shanghai 201306,
      China.
FAU - Chi, Aiping
AU  - Chi A
AUID- ORCID: 0000-0001-6804-2731
AD  - Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119,
      China.
LA  - eng
PT  - Journal Article
DEP - 20170322
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Amino Acids)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 387-79-1 (17-alpha-Hydroxypregnenolone)
RN  - A1L3K6161X (21-hydroxypregnenolone)
RN  - NGZ37HRE42 (Sphingosine)
SB  - IM
MH  - 17-alpha-Hydroxypregnenolone/*urine
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Behavior, Animal
MH  - Disease Models, Animal
MH  - Fatigue Syndrome, Chronic/physiopathology/*urine
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Gonadal Steroid Hormones/metabolism
MH  - Humans
MH  - *Physical Conditioning, Animal
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sphingosine/*urine
PMC - PMC5380834
EDAT- 2017/04/20 06:00
MHDA- 2017/05/02 06:00
CRDT- 2017/04/20 06:00
PHST- 2016/11/17 [received]
PHST- 2017/03/05 [accepted]
AID - 10.1155/2017/8182020 [doi]
PST - ppublish
SO  - Biomed Res Int. 2017;2017:8182020. doi: 10.1155/2017/8182020. Epub 2017 Mar 22.

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