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PMID- 28422972
DA  - 20170419
DCOM- 20170427
LR  - 20170427
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Challenges in enumeration of CTCs in breast cancer using techniques independent
      of cytokeratin expression.
PG  - e0175647
LID - 10.1371/journal.pone.0175647 [doi]
AB  - INTRODUCTION: Given the current postulated plasticity between epithelial and
      mesenchymal states of migratory cancer cells the detection of non-epithelial CTCs
      is an important scientific and clinical goal. METHODS: We used the
      filtration-based ISET technology to enrich circulating tumour cells (CTCs) in
      early breast cancer blood samples and identify them using a morphology-based
      immunocytochemistry (ICC) approach. RESULTS: We found greater numbers of putative
      CTCs by this approach than by the cytokeratin-based CellSearch technology, but a 
      high number of CTC false positives were identified in healthy volunteer samples
      which were not reduced in successive blood draws. Preliminary work using an
      oestrogen receptor (ER)-based multiplex ICC method in metastatic breast cancer
      ISET samples indicated a low number of ER+ CTCs even at this advanced stage.
      CONCLUSIONS: This work highlights the challenges in enumerating CTCs without
      conventional epithelial markers.
FAU - Castle, John
AU  - Castle J
AD  - Clinical and Experimental Pharmacology, Cancer Research UK Manchester Institute; 
      Manchester, United Kingdom.
FAU - Morris, Karen
AU  - Morris K
AD  - Clinical and Experimental Pharmacology, Cancer Research UK Manchester Institute; 
      Manchester, United Kingdom.
FAU - Pritchard, Susan
AU  - Pritchard S
AD  - Department of Histopathology, University Hospital of South Manchester;
      Manchester, United Kingdom.
FAU - Kirwan, Cliona C
AU  - Kirwan CC
AD  - Division of Molecular and Clinical Cancer Sciences, University of Manchester;
      Department of Academic Surgery, University Hospital of South Manchester,
      Manchester, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20170419
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Estrogen Receptor alpha)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics/metabolism
MH  - Breast Neoplasms/*diagnosis/genetics/metabolism/pathology
MH  - Case-Control Studies
MH  - Cell Count/instrumentation/*methods
MH  - Cell Separation/instrumentation/*methods
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Estrogen Receptor alpha/genetics/metabolism
MH  - False Positive Reactions
MH  - Female
MH  - Filtration/instrumentation/*methods
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratins/genetics/metabolism
MH  - Middle Aged
MH  - Neoplastic Cells, Circulating/metabolism/*pathology
EDAT- 2017/04/20 06:00
MHDA- 2017/04/28 06:00
CRDT- 2017/04/20 06:00
PHST- 2016/11/03 [received]
PHST- 2017/03/29 [accepted]
AID - 10.1371/journal.pone.0175647 [doi]
AID - PONE-D-16-43774 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 19;12(4):e0175647. doi: 10.1371/journal.pone.0175647.
      eCollection 2017.

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