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Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction.

Abstract Rotavirus group A (RVA) is considered the leading cause of pediatric diarrhea, responsible for the high burden of diarrheal diseases in sub-Saharan Africa. Despite recent studies, the existent data are scarce for some African countries like Angola, a country with one of the highest RVA-related death estimates. The aim of this study was to determine the RVA detection rate and circulating genotypes in children less than five years of age with acute gastroenteritis attended at the Bengo General Hospital in Caxito, Bengo province, Angola, before vaccine introduction.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos one
Publication Year Start




PMID- 28422995
OWN - NLM
STAT- In-Process
DA  - 20170419
LR  - 20170419
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Characterization of rotavirus infection in children with acute gastroenteritis in
      Bengo province, Northwestern Angola, prior to vaccine introduction.
PG  - e0176046
LID - 10.1371/journal.pone.0176046 [doi]
AB  - BACKGROUND: Rotavirus group A (RVA) is considered the leading cause of pediatric 
      diarrhea, responsible for the high burden of diarrheal diseases in sub-Saharan
      Africa. Despite recent studies, the existent data are scarce for some African
      countries like Angola, a country with one of the highest RVA-related death
      estimates. The aim of this study was to determine the RVA detection rate and
      circulating genotypes in children less than five years of age with acute
      gastroenteritis attended at the Bengo General Hospital in Caxito, Bengo province,
      Angola, before vaccine introduction. METHODS: Between September 2012 and December
      2013, 342 fecal specimens were collected from children enrolled. Positive samples
      for RVA by immunochromatographic rapid test were G and P-typed by hemi-nested
      type-specific multiplex PCR, and subgrouped for the VP6 gene. VP4 and VP7 genes
      from a subset of samples were sequenced for phylogenetic analysis. RESULTS:
      During the study period, a high RVA detection rate was registered (25.1%,
      86/342). The age group most affected by RVA infection includes children under 6
      months of age (p<0.01). Vomiting was highly associated with RVA infection (72.1%;
      p<0.001). From the 86 RVA-positive samples, 72 (83.7%) were genotyped. The most
      prevalent genotype was G1P[8] (34/72; 47.2%), followed by the uncommon G1P[6]
      (21/72; 29.2%), and G2P[4] (9/72; 12.5%). Only two G-types were found: G1 (60/72;
      83.3%) and G2 (11/72; 15.3%). Among the P-genotypes, P[8] was the most prevalent 
      (34/72; 47.2%), followed by P[6] (22/72; 30.6%) and P[4] (9/72; 12.5%). In the
      phylogenetic trees, the identified G and P-types clustered tightly together and
      with reference sequences in specific monophyletic groups, with highly significant
      bootstrap values (>/=92%). CONCLUSION: This pre-vaccination study revealed, for
      the first time for Bengo province (Angola), the RVA genotype profile, including
      phylogenetic relationships, and a high RVA detection rate, supporting the
      immediate introduction of a RVA vaccine in the national immunization programme.
FAU - Gasparinho, Carolina
AU  - Gasparinho C
AD  - Centro de Investigacao em Saude de Angola (CISA), Caxito, Provincia do Bengo,
      Angola.
FAU - Piedade, Joao
AU  - Piedade J
AD  - Global Health and Tropical Medicine (GHTM), Unidade de Microbiologia Medica,
      Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa
      (UNL), Lisbon, Portugal.
FAU - Mirante, Maria Clara
AU  - Mirante MC
AD  - Centro de Investigacao em Saude de Angola (CISA), Caxito, Provincia do Bengo,
      Angola.
FAU - Mendes, Cristina
AU  - Mendes C
AD  - Global Health and Tropical Medicine (GHTM), Unidade de Microbiologia Medica,
      Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa
      (UNL), Lisbon, Portugal.
FAU - Mayer, Carlos
AU  - Mayer C
AD  - Hospital Geral do Bengo, Caxito, Provincia do Bengo, Angola.
FAU - Vaz Nery, Susana
AU  - Vaz Nery S
AD  - Centro de Investigacao em Saude de Angola (CISA), Caxito, Provincia do Bengo,
      Angola.
AD  - Research School of Population Health, The Australian National University,
      Canberra, Australia.
FAU - Brito, Miguel
AU  - Brito M
AD  - Centro de Investigacao em Saude de Angola (CISA), Caxito, Provincia do Bengo,
      Angola.
AD  - Escola Superior de Tecnologia da Saude de Lisboa, Lisbon, Portugal.
FAU - Istrate, Claudia
AU  - Istrate C
AUID- ORCID: http://orcid.org/0000-0003-4706-7598
AD  - Global Health and Tropical Medicine (GHTM), Unidade de Microbiologia Medica,
      Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa
      (UNL), Lisbon, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20170419
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
EDAT- 2017/04/20 06:00
MHDA- 2017/04/20 06:00
CRDT- 2017/04/20 06:00
PHST- 2016/11/03 [received]
PHST- 2017/04/04 [accepted]
AID - 10.1371/journal.pone.0176046 [doi]
AID - PONE-D-16-43791 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 19;12(4):e0176046. doi: 10.1371/journal.pone.0176046.
      eCollection 2017.

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