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Immunocytochemistry Improving the Diagnosis of Trichomonas vaginalis Infections.

Abstract The aim of this study was to evaluate the immunocytochemistry (ICC) to diagnose trichomoniasis, particularly asymptomatic infections. By culture serial dilutions, ICC was able to detect 1 trophozoite/mL, while the culture was positive up to 100 trophozoites/mL. The ICC in vivo detection capability was assessed in vaginal secretions of mice experimentally infected and in vaginal swabs from asymptomatic HIV-positive pregnant women compared with culture. All vaginal secretion samples from mice were positive according to both methods. Swabs from fifty-five asymptomatic women were positive in four (7.27%) of them by culture. Beyond these four, another ten (25.45%) women were positive by immunocytochemistry, proving their higher sensitivity (p = 0.002), noticing 3.5 times more positives. ICC had better performance in both successive dilutions as in asymptomatic women, showing higher sensitivity and specificity. In this way, its facility of execution and cost-effectiveness support its practicality, as a routine procedure to diagnose trichomoniasis not only when the parasite load is lower but probably in all clinical scenarios.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title biomed research international
Publication Year Start




PMID- 28424786
OWN - NLM
STAT- MEDLINE
DA  - 20170420
DCOM- 20170508
LR  - 20170508
IS  - 2314-6141 (Electronic)
VI  - 2017
DP  - 2017
TI  - Immunocytochemistry Improving the Diagnosis of Trichomonas vaginalis Infections.
PG  - 5642535
LID - 10.1155/2017/5642535 [doi]
AB  - The aim of this study was to evaluate the immunocytochemistry (ICC) to diagnose
      trichomoniasis, particularly asymptomatic infections. By culture serial
      dilutions, ICC was able to detect 1 trophozoite/mL, while the culture was
      positive up to 100 trophozoites/mL. The ICC in vivo detection capability was
      assessed in vaginal secretions of mice experimentally infected and in vaginal
      swabs from asymptomatic HIV-positive pregnant women compared with culture. All
      vaginal secretion samples from mice were positive according to both methods.
      Swabs from fifty-five asymptomatic women were positive in four (7.27%) of them by
      culture. Beyond these four, another ten (25.45%) women were positive by
      immunocytochemistry, proving their higher sensitivity (p = 0.002), noticing 3.5
      times more positives. ICC had better performance in both successive dilutions as 
      in asymptomatic women, showing higher sensitivity and specificity. In this way,
      its facility of execution and cost-effectiveness support its practicality, as a
      routine procedure to diagnose trichomoniasis not only when the parasite load is
      lower but probably in all clinical scenarios.
FAU - Fonseca, Thaisa Helena S
AU  - Fonseca THS
AUID- ORCID: 0000-0002-8696-9925
AD  - Department of Parasitology, Institute of Biological Sciences, Universidade
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Oliveira, Fabricio M Silva
AU  - Oliveira FMS
AD  - Department of Pathology, Institute of Biological Sciences, Universidade Federal
      de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Alacoque, Marina
AU  - Alacoque M
AD  - Department of Parasitology, Institute of Biological Sciences, Universidade
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Rocha, Marcella Israel
AU  - Rocha MI
AD  - Department of Gynecology and Obstetrics, Universidade Federal de Minas Gerais,
      Belo Horizonte, MG, Brazil.
FAU - Leite, Henrique Vitor
AU  - Leite HV
AD  - Department of Gynecology and Obstetrics, Universidade Federal de Minas Gerais,
      Belo Horizonte, MG, Brazil.
FAU - Santos, Joseph F Guimaraes
AU  - Santos JFG
AD  - IPSEMG Hospital, Belo Horizonte, MG, Brazil.
FAU - Busatti, Haendel G N O
AU  - Busatti HGNO
AD  - Universidade de Itauna, Itauna, MG, Brazil.
FAU - Caliari, Marcelo Vidigal
AU  - Caliari MV
AD  - Department of Pathology, Institute of Biological Sciences, Universidade Federal
      de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Gomes, Maria Aparecida
AU  - Gomes MA
AUID- ORCID: 0000-0002-7263-5721
AD  - Department of Parasitology, Institute of Biological Sciences, Universidade
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170323
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Female
MH  - HIV Infections/complications/parasitology
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - Mice, Inbred BALB C
MH  - Trichomonas Infections/complications/*diagnosis
MH  - Trichomonas vaginalis/*physiology
PMC - PMC5382293
EDAT- 2017/04/21 06:00
MHDA- 2017/05/10 06:00
CRDT- 2017/04/21 06:00
PHST- 2016/12/29 [received]
PHST- 2017/02/14 [revised]
PHST- 2017/02/26 [accepted]
AID - 10.1155/2017/5642535 [doi]
PST - ppublish
SO  - Biomed Res Int. 2017;2017:5642535. doi: 10.1155/2017/5642535. Epub 2017 Mar 23.

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